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《蚕业科学》2015,(3)
筛选适合川桑(Morus notabilis)、滇桑(Morus yunnanensis)、药桑(Morus nigra)、印度桑K2(Morus indica cv.K2)无菌幼苗茎段继代和生根培养的培养基配方,建立4种特殊桑树种质资源的离体组织培养方法。以供试桑种质资源无菌幼苗带腋芽的茎段为外植体,通过对培养基添加激素浓度的优化,筛选出药桑的增殖芽继代培养基为MS+1 mg/L 6-BA+0.1 mg/L NAA,滇桑的增殖芽继代培养基为MS+0.1 mg/L TDZ,川桑的增殖芽继代培养基为MS+1 mg/L 6-BA+0.2 mg/L NAA,印度桑K2的增殖芽继代培养基为MS+0.05 mg/L TDZ。4份桑种质资源的无菌幼苗茎段继代培养45 d后,产生的增殖芽数目达1.6~6.8个,再生幼苗株高可达4 cm以上,分别转移至筛选的生根培养基MS+0.5 mg/L IBA或MS+1 mg/L IBA中培养。除川桑再生幼苗的生根率(41.7%~45.8%)和移栽成活率(40%~50%)较低外,其他3份桑种质资源再生幼苗的生根率均可达90%以上,移栽成活率为100%。试验结果证实不同桑种质资源最适合的组织培养体系存在差异,并且培养基中激素浓度小幅变化都会对其繁殖系数造成显著影响。 相似文献
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绶草的组织培养与快速繁殖研究 总被引：32，自引：0，他引：32
以野生花卉植物绶草的肉质根、幼叶、花序轴为外植体,建立组织培养快繁体系.试验结果显示:绶草组织培养较理想的外植体材料为花序轴,诱导率达到83%:最适诱导培养基为MS+3.0%蔗糖+0.75%琼脂+6-BA 1.0 mg/L+NAA0.1 mg/L;最适增殖培养基为MS+3.0%蔗糖+0.75%琼脂+6-BA 2.0 mg/L+NAA 0.2 mg/L+椰汁100g/L;最适生根培养基为1/2 MS+2.0%蔗糖+0.5%琼脂+IBA 0.5 mg/L+NAA 0.5 mg/L+活性炭0.5 g/L. 相似文献
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为了降低陇东海棠离体初代培养中的褐化现象、提高培养成功率,研究了外植体类型、酒精消毒、低温处理、防褐化剂、暗培养时间和6-BA浓度对陇东海棠在组织培养中外植体褐化的影响。结果表明：以带芽茎段为外植体,用0.1% HgCl2灭菌处理12min,接种在MS 6-BA0.5～1.0mg/L IBA0.2 mg/L（pH=5.8）添加0.5～2g/LPVP的培养基中,低温暗处理5d,对防止外植体褐化效果最佳。 相似文献
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以桑品种育-711的叶片、芽尖、叶柄、茎段为材料,探讨不同外植体、不同培养基和激素配比等因素对桑树愈伤组织的诱导、继代培养以及悬浮细胞培养的影响,并对桑树悬浮细胞的生长曲线进行监测,初步建立桑树细胞悬浮培养体系。试验结果表明:叶片是诱导桑树愈伤组织的理想外植体,愈伤组织最佳诱导条件为MS培养基中添加1.5 mg/L 6-BA+0.5 mg/L2,4-D+0.5 mg/L NAA的激素组合,在此培养条件下的诱导率达90%以上;继代培养最优条件为MS培养基中添加1.0 mg/L6-BA+0.5 mg/L 2,4-D+0.5 mg/L NAA,在此培养条件下继代,愈伤组织生长旺盛,存活率达到100%,培养12 d进入对数生长期,愈伤组织的鲜质量在此期间增加6倍;悬浮细胞在MS液体培养基中添加1.5 mg/L 6-BA+0.5 mg/L 2,4-D+0.1 mg/L NAA的培养条件下,细胞增殖较快,生长曲线呈"S"型,活细胞数量在悬浮培养第16天时达到峰值。以桑树叶片作为外植体诱导愈伤组织及初步建立的悬浮细胞系,有助于进一步研发桑树组织培养物次生代谢产物的生产技术。 相似文献
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杨梅品种‘浪荡子’是优良的地方品种。本文以茎尖、茎段为材料,对‘浪荡子’杨梅进行了组培快繁技术的研究。结果表明：以春季3～5月份取样为最佳时期;外植体先用75%乙醇浸泡50～60s,再用0.1%升汞处理7min这个消毒处理组合效果最好;在WPM 0.15 mg/ L 6-BA 0.03 mg/ L NAA 1.0 g/ L PVP培养基上芽萌动和茎伸长效果最好;在WPM 1.2 mg/ L 6-BA 0.1 mg/ L NAA 0.5 mg/ LGA3 1.0 g/ L PVP培养基上新芽增殖的效果最好。 相似文献
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复序橐吾组培再生体系研究 总被引：3，自引：1，他引：2
用复序橐吾(Ligularia jaluensis Kom.)种子获得无菌苗,取其叶片为外植体,以MS培养基为基础,通过添加不同浓度和种类的细胞分裂素及生长素,目的是筛选出叶片组织培养的最适培养基。结果表明:种子较好的灭菌时间为75%酒精30 s+1‰升汞4min;初代培养基为MS+6-BA0.5mg/L+2,4-D1.0mg/L,由此获得的愈伤组织致密、颜色绿,具有很好的诱导效果,其诱导率达到90%;继代增殖的最适培养基为MS+6-BA1.0mg/L+NAA0.1mg/L,其增殖倍数高达3.9,并且苗生长状况良好;生根最适培养基为:1/2MS+NAA 0.5mg/L,生根率达100%。 相似文献
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《中兽医医药杂志》2016,(3)
以药用植物紫花地丁叶片和叶柄为外植体,经过不同灭菌处理等,进行紫花地丁组织培养与快繁技术的研究。结果表明,紫花地丁叶片用70%乙醇处理20 s、0.1%Hg Cl2处理6 min灭菌效果最佳,而叶柄的最佳灭菌条件为70%乙醇40 s+0.2%HgCl_24 min;叶片最适愈伤组织诱导培养基为2/3MS+2,4-D 0.2 mg/L+6-BA 0.8 mg/L,叶柄在MS+2,4-D 0.2 mg/L+6-BA 0.6 mg/L培养基上的诱导分化效果最好;MS+2,4-D 0.6 mg/L+6-BA 0.8 mg/L和MS+2,4-D 0.4 mg/L+6-BA 0.6 mg/L分别为叶片、叶柄诱导丛生芽和继代增殖的最佳培养基;最适生根培养基是1/2MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L+IBA 0.2 mg/L,生根率89%,移栽后,成活率高达95%。本研究成功建立了紫花地丁组织培养快繁体系,为其药用价值的进一步开发利用奠定了基础。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献