共查询到20条相似文献，搜索用时 78 毫秒
1.
《饲料工业》2016,(4)
试验通过在种子液培养基中添加腐植酸钠,探讨腐植酸钠对酵母菌发酵特性的影响。将腐植酸钠按不同比例添加到酵母菌种子液培养基中,以活菌数为指标筛选腐植酸钠的最适添加量;再将含有最适比例腐植酸钠的酵母菌种子液接种到稻壳粉、精料、豆粕和统糠4种固态培养基中,测定发酵产物中的活菌数,可溶性糖、可溶性蛋白和真蛋白质含量等指标。试验结果为:种子液中添加1%~4%的腐植酸钠对酵母菌的生长有明显促进作用,当腐植酸钠添加量为2%时,酵母菌活菌数最高(P0.05)。种子液中添加腐植酸钠可明显改善酵母菌在固态培养基中的发酵特性:种子液中添加腐植酸钠可显著提高4种固态发酵基质中酵母菌的活菌数,且在豆粕中提高量最大(P0.05);可显著降低精料、豆粕、统糠发酵物中的可溶性糖含量,提高稻壳粉中可溶性糖的含量,其中统糠中可溶性糖含量降低最为明显(P0.05);可显著提高精料发酵产物中可溶性蛋白质含量(P0.05),对稻壳粉、豆粕和统糠三种培养基中可溶性蛋白质含量的影响不显著(P0.05);可显著增加稻壳粉、精料、豆粕和统糠4种培养基中真蛋白质含量(P0.05),其增加率由高到低依次为:稻壳粉、精料、统糠、豆粕。结果显示,种子液中添加腐植酸钠可显著影响酵母菌固态发酵的特征,进而提高发酵效果,改善发酵饲料品质。 相似文献
2.
本试验旨在分离鉴定甘肃地区奶牛场饲料中的酿酒酵母菌,进而研究酿酒酵母菌发酵液的体外抑菌活性。通过YPD固体培养基对采集的5份饲草和5份饲料样品中的酵母菌进行分离,分别进行生化鉴定和26SrDNA测序鉴定,进而检测酿酒酵母发酵液的体外抑菌活性。结果表明,从样品中分离出4种共12株酵母菌,分别为3株酿酒酵母菌(Y1、Y7和Y11)、4株汉逊德巴利酵母菌、3株浅黄隐球酵母菌和2株戴尔有孢圆酵母菌;3株酿酒酵母菌的发酵液对奶牛乳房炎金黄色葡萄球菌、大肠杆菌和无乳链球菌的抑菌活性存在差异,其中酵母菌Y7和Y11的发酵液对3种病原菌均具有抑菌活性,而Y1的发酵液只对大肠杆菌具有抑制作用。本试验为进一步筛选性能优异的酿酒酵母菌用于微生态制剂研发奠定了基础。 相似文献
3.
研究旨在从新疆库车馕面团中分离筛选能够高效降解淀粉的酵母菌并对其生理生化特性进行研究。试验利用不同的培养基,从新疆库车县不同馕房采集的馕面团中分离酵母菌,测定酵母菌总数,并对具有降解淀粉功能的菌株进行淀粉降解试验、形态学观察、生理生化及分子鉴定。结果分离得到31株酵母菌,其中8株菌株具有淀粉降解能力,通过进一步筛选获得2株高效降解淀粉的功能菌株Y2-2和Y3-1,降解率分别为81.07%和88.12%。经生理生化试验和26S rDNA D1/D2区域序列鉴定,这2株菌属于酿酒酵母,是该样品的特殊酵母品系,试验将为以淀粉做原料的工业生产和高蛋白质性新饲料添加剂的开发提供优质菌种。 相似文献
4.
5.
减少瘤胃甲烷排放措施的研究已成为当前的研究热点之一。本研究以酒、面包和畜禽专用酿酒酵母产品为原材料,从中分离到三株酵母菌,将其制成冻干粉,通过体外产气试验,筛选出了一株抑制瘤胃甲烷排放效果最为显著的酵母菌株。然后以该酵母菌株作为研究对象,进行分子生物学鉴定;采用分光光度计比浊法测定该酵母菌株的生长曲线,确定菌体生长规律;并以此为依据,设计单因素试验考察该酵母菌株最适宜的生长条件。结果表明,分离得到的酵母菌株均为酿酒酵母saccharomyces cerevisiae;抑制瘤胃体外甲烷排放效果最显著的1株酵母菌分离自面包中;该酿酒酵母菌株的对数生长期为8～24h,其最佳培养条件为:生长温度30℃,pH 5.5,培养基中葡萄糖的浓度4.0%。 相似文献
6.
7.
8.
9.
10.
11.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
12.
13.
14.
Reasons for performing study: Clinical experiences indicate that centesis of the lateral compartment of the femorotibial joint is often unsuccessful. Objective: To determine the accuracy of 2 published and 2 unpublished techniques of centesis of the lateral compartment of the femorotibial joint. Hypothesis: It was hypothesised that a drug can be administered into the lateral compartment of the femorotibial joint via a diverticulum of this joint that surrounds the medial aspect of the long digital extensor tendon and that this technique is more accurate than described techniques of centesis of this compartment. Methods: Twenty‐four stifles of 12 horses were divided equally into 4 groups and a radiocontrast medium injected into the lateral compartment of the femorotibial joint of each group using a hypodermic needle inserted: 1) caudal to the lateral patellar ligament and proximal to the tibial plateau, 2) caudal to the long digital extensor tendon and proximal to the tibial plateau, 3) between the long digital extensor tendon and bone of the extensor groove of the tibia or 4) directly through the long digital extensor tendon until it contacted bone. Twelve veterinary students who had no experience using any of these techniques performed the injections. Accuracy of each technique was determined by examining radiographs obtained after injecting the contrast medium. Results: The most successful technique for arthrocentesis was by inserting a needle through the long digital extensor tendon. This approach was successful in all attempted cases, whilst other techniques had lower rates of success. Conclusions: The lateral compartment of the femorotibial joint can be accessed accurately by inserting a needle through the long digital extensor tendon as it lies within the extensor groove. Other techniques may not be as accurate for clinicians inexperienced in arthrocentesis of the lateral compartment of the femorotibial joint. 相似文献
15.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
16.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
Marioni-Henry K Vite CH Newton AL Van Winkle TJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):851-858
A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified. 相似文献
18.
根据JJF1059-1999《测量不确定度评定与表示》建立了高效液相色谱法测定鸡肉中土霉素残留测量的不确定度评定方法,评定过程包括建立测量程序、分析不确定度来源、对不确定度分量进行量化。将不确定度归类为两大类。随机效应导致的不确定度可以通过一系列具有统计意义的重复观测值进行A类评定;系统效应导致的不确定度通过各测量器具、设备的说明书、校准证书提供的参数进行B类评定。本方法鸡肉中土霉素残留量的相对合成标准不确定度约为3.9%。 相似文献
19.
本试验旨在研究两种绿头鸭（白羽绿头鸭和绿头鸭）肌肉营养组成和价值,为绿头鸭肉产品的开发利用提供基础数据和理论依据。选择白羽绿头鸭和绿头鸭60只（每个品种各30只,公母各半）,依照国家标准测定胸肌粗蛋白质、粗脂肪、胆固醇含量及氨基酸、脂肪酸、微量元素和维生素的组成和含量,并对胸肌进行氨基酸评价及脂肪酸营养价值评价。结果显示,白羽绿头鸭肌肉粗蛋白质含量显著低于绿头鸭（P<0.05）;两种绿头鸭肌肉中均检测到17种含量大于0.01%的氨基酸,其中白羽绿头鸭肌肉苏氨酸、组氨酸、丝氨酸和脯氨酸含量显著高于绿头鸭（P<0.05）,赖氨酸、谷氨酸和精氨酸含量显著低于绿头鸭（P<0.05）;检测到13种含量均大于0.01%的脂肪酸,其中白羽绿头鸭硬脂酸和油酸含量显著低于绿头鸭（P<0.05）;检测到9种矿物质元素（钠、镁、钾、钙、锰、铁、铜、锌和硒）,两种绿头鸭差异不显著（P>0.05）;检测到8种维生素,白羽绿头鸭肌肉维生素B1含量显著高于绿头鸭（P<0.05）,维生素D和维生素E含量显著低于绿头鸭（P<0.05）。两种绿头鸭肌肉氨基酸比例接近世卫组织推荐的理想模式,富含人体所需的矿物质元素和维生素,具有广阔的开发利用前景。 相似文献
20.
以5个金柑属品种、枳壳、软枝酸橘及广西土柠檬实生苗为材料,进行复水成活率、旱害指数、叶片相对含水量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、丙二醛（MDA）、脯氨酸等生理指标及隶属函数的综合分析,为柑橘抗性砧木品种选育提供参考。结果表明：胁迫20d复水后的成活率融安滑皮金柑和长寿金柑最高,为100%,软枝酸橘最低,为88.9%;随着干旱胁迫加剧,各品种的旱害指数呈上升趋势,长寿金柑的旱害指数最低,为0.25,软枝酸橘的旱害指数最高,为0.61;从叶片相对含水量的结果来看,长寿金柑的变化幅度最小,软枝酸橘的变化幅度最大,在第18d时分别为61.19%和37.85%;3种保护酶之间相互协同作用减轻植株受害程度,其活性变化幅度与丙二醛和脯氨酸的含量变化相似。利用隶属函数法综合分析,得出各品种的抗旱性强弱依次为长寿金柑>枳壳>融安滑皮金柑>融安金柑>山金柑>广西土柠檬>金枣>软枝酸橘。 相似文献