共查询到20条相似文献，搜索用时 78 毫秒
1.
《中国兽医学报》2016,(5):795-800
研究了猴头菇多糖协同ConA对小鼠体外脾淋巴细胞分泌Th1、Th2细胞因子的量及mRNA表达量的影响,旨在探讨其协同免疫调节机制。用ELISA法检测HEP协同ConA刺激脾淋巴细胞细胞上清液中Th1、Th2细胞因子的量;RT-PCR法检测Th1、Th2细胞因子和转录因子mRNA的表达。结果显示,HEP在25~400mg/L质量浓度范围内能显著协同ConA刺激T细胞亚群Th1细胞因子(IL-2、IFN-γ、TNF-α)和Th2细胞因子(IL-4、IL-6)的分泌及mRNA的表达(P0.05),并显著促进转录因子(T-bet、Gata-3)mRNA的表达(P0.05)。结果表明,HEP能协同ConA促进小鼠脾淋巴细胞Th1、Th2细胞因子的分泌及基因的表达,通过调节Th1/Th2平衡,发挥双重免疫调节作用。 相似文献
2.
为了研究硼对小鼠脾淋巴细胞增殖及细胞因子的影响,分别采用CCK-8、ELISA及qPCR技术检测不同浓度硼协同刀豆凝集素(concanavalin A,ConA)或脂多糖(lipopolysaccharide,LPS)对小鼠脾淋巴细胞增殖、细胞因子分泌及其mRNA表达的影响。结果显示,当硼协同ConA作用于脾淋巴细胞时,随着硼浓度的增加,细胞存活率呈先上升后下降的趋势;IL-2的分泌量逐渐下降,IL-6的分泌量没有显著变化;IL-2、IFN-γ及IL-6 mRNA的相对表达量有所增加,IL-10则表现为逐渐下降的趋势。当硼协同LPS作用于脾淋巴细胞时,随着硼浓度的增加,细胞存活率也呈先上升后下降的趋势;显著促进了IL-2、IL-6的分泌(P<0.05);硼浓度为1,10,50 mmol/L时,IL-2及IL-10 mRNA的相对表达量均显著低于LPS单独刺激组(P<0.05),IL-6 mRNA的相对表达量先下降后上升,IFN-γ则表现为逐渐下降。结果说明低浓度的硼对脾淋巴细胞的增殖及细胞因子的分泌起促进作用,高浓度的硼则对细胞产生毒性作用且在一定程度上抑制相关细胞因子的分泌,且硼对脾淋巴细胞的细胞因子表达也起到了一定的调控作用。 相似文献
3.
4.
5.
研究刺五加多糖(ASPS)对鸡淋巴细胞体外增殖及细胞因子mRNA表达水平的影响。将不同浓度的ASPS添加到体外分离培养的血液淋巴细胞中，采用噻唑蓝(MTT)法检测淋巴细胞增殖变化，以实时荧光定量PCR方法检测淋巴细胞细胞因子IFN-γ、IL-2、IL-4、IL-10、IL-1β、IL-6、iNOS和TNF-αmRNA表达水平。结果显示，ASPS可以单独或协同植物血凝素(PHA)或脂多糖(LPS)促进淋巴细胞体外增殖，并且ASPS在质量浓度为200μg/mL时效果最佳。另外，ASPS能够促进淋巴细胞细胞因子mRNA的表达，当200μg/mL的ASPS与淋巴细胞体外共培养至24 h时，IFN-γ、IL-2、IL-1β、IL-6、iNOS和TNF-α的mRNA表达量均最高，而IL-4和IL-10 mRNA的相对表达量则以ASPS质量浓度为400μg/mL和培养时间48 h条件下最高，说明IL-4和IL-10 mRNA的表达滞后，而且呈剂量依赖性。结果表明，ASPS能够提高鸡淋巴细胞的体外增殖功能，促进Th1和Th2细胞因子分泌，这为进一步揭示刺五加多糖的免疫调节机制奠定了基础。 相似文献
6.
妊娠是一个复杂的生理过程,受到神经、免疫和内分泌的共同调节。作为半自体抗原的胎儿不被母体排斥是子宫局部免疫水平调节的结果。其中激素对辅助性淋巴细胞(Th)发生、分化的调节作用愈来愈受到重视。辅助T淋巴细胞能分化为不同的亚型(Th1和Th2),孕酮可促进Th2型细胞因子(IL-4,IL-5)的产生,有利于妊娠的正常发生;松弛肽促进Th1型细胞因子(IFN-γ)的产生,对妊娠有害。在母胎界面存在激素-细胞因子网络,淋巴细胞产生的细胞因子还能够影响其他细胞因子的产生。由Th2样细胞产生的,对胚泡着床具有非常重要作用的白血病抑制因子(LIF),其表达受Th1细胞诱导因子的下调,如IL-12,IFN-α,IFN-γ,受IL-4和孕酮(诱导T淋巴细胞向Th2细胞分化)的上调。激素通过调控淋巴细胞分泌的细胞因子来调控免疫系统,保持一个动态的平衡,有利于维持妊娠。 相似文献
7.
《畜牧兽医学报》2015,(6)
本试验以绵羊外周血淋巴细胞为研究对象,研究沙葱黄酮对绵羊外周血淋巴细胞增殖率,IL-2、IL-4、IFN-γ基因mRNA表达量的影响,为全面揭示沙葱黄酮的免疫调节活性机制和相关产品的开发提供试验依据。试验采用MTT法研究沙葱黄酮对绵羊外周血淋巴细胞增殖率的影响,使用RT-PCR法研究沙葱黄酮对绵羊外周血淋巴细胞IL-2、IL-4、IFN-γ基因mRNA表达量的影响。结果显示,沙葱黄酮具有促进绵羊外周血淋巴细胞增殖的作用,对绵羊外周血淋巴细胞IL-2mRNA表达无明显调节作用,可上调IFN-γmRNA表达,下调IL-4mRNA表达。综上,沙葱黄酮具有促进绵羊外周血淋巴细胞增殖及免疫调节活性的作用。 相似文献
8.
9.
《中国畜牧兽医》2020,(4)
试验旨在探究UBC13蛋白对支气管哮喘小鼠体内Th2、Th17细胞极化的影响。18只BALB/c小鼠随机均分为3组:PBS组、哮喘模型(OVA)组和UBC13蛋白(UBC13)组,OVA组和UBC13组采用卵清蛋白(OVA)和脂多糖(LPS)进行致敏和雾化建立哮喘模型,其中UBC13组于每次雾化前0.5 h腹腔注射100μg UBC13蛋白。末次雾化激发24 h后处死小鼠,采集样品,通过HE染色观察肺脏切片病理学变化、PAS染色观察气道黏液的分泌,ELISA法检测血清IgE浓度和肺支气管肺泡灌洗液(BALF)上清中IL-4、IL-5和IL-17A的水平,实时荧光定量PCR法检测肺脏Th2型相关因子IL-4、IL-5、IL-13、IFN-γmRNA和Th17型细胞因子IL-1β、IL-6、IL-17A、IL-23 mRNA的相对表达量。结果显示,试验成功建立了哮喘模型,与PBS组相比,OVA组IgE浓度极显著上升(P0.01),肺脏病理切片炎性细胞浸润和中性黏液分泌现象明显,肺脏中IL-1β、IL-4、IL-6、IL-13和IL-17A mRNA相对表达量均极显著升高(P0.01),IL-5和IL-23 mRNA相对表达量显著升高(P0.05),IFN-γmRNA相对表达量极显著下降(P0.01),BALF中IL-4、IL-5和IL-17A的水平极显著上升(P0.01);与OVA组相比,UBC13组IgE浓度显著降低(P0.05),病理切片炎性细胞浸润现象减轻、黏液分泌减少,肺脏IL-1β、IL-4、IL-17A、IL-13和IL-23 mRNA相对表达量均显著下降(P0.05),IFN-γmRNA相对表达量显著升高(P0.05);IL-6 mRNA相对表达量极显著下降(P0.01),BALF中IL-5的水平极显著下降(P0.01),IL-4的水平显著下降(P0.05),IL-17A的水平无显著差异(P0.05)。由此可见,UBC13蛋白能下调Th2和Th17型细胞因子的表达量,影响哮喘模型Th2和Th17细胞的极化。 相似文献
10.
11.
12.
13.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
16.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
18.
19.
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献