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《黑龙江畜牧兽医》2015,(13)
为了揭示建昌黑山羊主要生殖激素在繁殖期不同阶段的分泌规律,试验选择12头建昌黑山羊健康母羊,分别于妊娠早期(妊娠1~90 d)、妊娠晚期(妊娠91~150 d)、泌乳期和空怀期采集血样,分离血清后采用酶联免疫法(ELISA)进行检测,根据标准回归方程得到繁殖期内各阶段雌二醇(E2)、孕酮(P4)、促卵泡激素(FSH)、促黄体素(LH)浓度。结果表明:雌二醇浓度在妊娠期达到峰值,分娩后急剧下降,空怀期比妊娠晚期下降了50.6%,差异显著(P0.05);孕酮浓度呈递减趋势,妊娠早期与空怀期相比差异显著(P0.05),妊娠早期与泌乳期相比差异显著(P0.05),空怀期和泌乳期比妊娠早期分别下降了44.9%和40.7%;促卵泡激素和促黄体素各时期相比均差异不显著(P0.05),两者变化规律一致。说明在建昌黑山羊繁殖周期中,雌二醇、孕酮、促卵泡激素、促黄体素对母羊妊娠、泌乳及间情等繁殖行为具有十分重要的作用。 相似文献
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母猪妊娠早期外周血出现的17β雌二醇,可能是建立母体妊娠识别及维持胚胎正常发育同孕酮一样是不可缺少的。妊娠早期(40～45天)高水平孕酮可作为早期妊娠诊断的指标。高于10毫微克/毫升可判断为妊娠,低于此值可判断为不孕。对46头配种后40～45天的长白、苏白杂种母猪,14头产后屡配不孕猪外周血浆孕酮和17β雌二醇测定(放射免疫 RIA法),经直肠检查和屠宰验证结果:妊娠组孕酮(n=39)和17β雌二醇(n=19)含量分别为19.47±7.23毫微克/毫升和31.82±24.40微微克/毫升。未妊娠及产后不孕组分别为7.87±4.25毫微克/毫升和12.72±12.16微微克/毫升。妊娠组比非妊娠及产后不孕组孕酮和17β雌二醇水平均高,经统计处理,差异分别为极显菩(P<0.01)和显著(P<0.05)。一例卵泡囊肿猪(84730号)血浆孕酮值为37.65毫微克/毫升,高出妊娠组平均值(19.47毫微克/毫升),为此可考虑用促性腺激素释放激素(G_n,RH)或促黄体素(LH)治疗。 相似文献
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《养猪》2019,(3)
为研究加强型复合添加剂(诸姆安Ⅱ)对母猪繁殖性能及生殖激素的影响,选择体况良好、配种日期相近的长大二元健康经产母猪40头,随机等分为2组。对照组母猪按猪场常规方法饲喂基础饲粮,试验组母猪在基础饲粮中添加"诸姆安Ⅱ"产品,配种后至妊娠85 d每头每天2.5 g,妊娠86 d至哺乳全期每头每天5 g。跟踪测评各组母猪繁殖性能及生殖激素的变化。研究结果表明:1)与对照组相比,试验组母猪所繁殖仔猪初生个体重、初生窝重、断乳个体重分别提高6.34%、15.92%、7.48%,差异显著(P0.05)。试验组母猪产仔数、产活仔数也分别提高5.37%、8.79%,但差异不显著(P0.05)。2)与对照组相比,试验组母猪泌乳力(21日龄窝重)提高18.88%(P0.05),初乳蛋白质含量提高13.64%(P0.05),IgG含量有提高趋势(提高10.50%,P0.05);母猪断乳7 d内发情率提高6.67个百分点,发情间隔平均缩短0.87 d(P0.05)。3)生殖激素测定结果表明,与对照组相比,试验组母猪产后第14天血清催乳素、促黄体素和雌二醇含量分别提高29.42%、28.04%和14.37%(P0.05);母猪断乳后第5天血清孕酮含量降低12.98%(P0.05),促卵泡素、促黄体素和雌二醇含量分别提高30.52%、17.05%和11.23%(P0.05)。研究证明,经产母猪饲喂添加"诸姆安Ⅱ"能够改善其生殖内分泌机能,有效促进妊娠期胎儿的生长发育,哺乳期泌乳性能以及仔猪生长性能得到提升,母猪断乳后发情配种等再繁殖性能也得到相应改善。 相似文献
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富铜酵母对西门塔尔牛发情周期生殖激素分泌的影响 总被引：1，自引：0，他引：1
选用16头18月龄、体况良好、体质量390 kg的西门塔尔牛育成母牛,采用完全随机分组设计分为4组,研究富铜酵母(0、8、16、24 mg/kg)对发情周期外周血清促黄体素、促卵泡素、孕酮和雌二醇分泌规律的影响.结果显示,8 mg/kg组和16 mg/kg组显著提高了发情周期促黄体素、促卵泡素、孕酮和雌二醇水平(P<0.05);24 mg/kg组有提高趋势,但与对照组比差异不显著(P>0.05).结果表明,添加富铜酵母以8～16 mg/kg对发情周期生殖激素分泌有促进作用,以8 mg/kg为佳,兼顾基础日粮的含铜量,建议日粮铜水平为13.87 mg/kg. 相似文献
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试验旨在研究生物发酵饲料对三元杂交母猪胎盘效率、血清生殖激素和免疫力的影响。选取第2胎、背膘厚（18.23±0.36）mm、健康的杜×长×大[美系杜洛克♂×（丹系长白♂×丹系大约克♀）]三元杂交母猪60头，随机分成4组，每组15个重复（栏），每个重复1头母猪。对照组饲喂基础饲粮，其他3个试验组分别在基础饲粮中添加5%、10%、15%生物发酵饲料，试验期从妊娠70 d至分娩。结果表明：与对照组相比，10%和15%生物发酵饲料组母猪妊娠104 d时血清雌二醇（E2）含量降低（P<0.05），促黄体素（LH）、促卵泡素（FSHB）、孕酮（PROG）、催乳素（PRL）含量提高（P<0.05），胎盘重量提高（P<0.05）；与对照组相比，5%、10%和15%生物发酵饲料组妊娠母猪血清IgA、IgG和IgM含量提高（P<0.05），产程缩短（P<0.01），窝均产活仔数提高（P<0.01），初生窝重提高（P<0.05）；不同添加比例生物发酵饲料对窝均产仔数、仔猪体长和体重指数影响差异均不显著，胎盘效率有提高趋势（P>0.05）。本试验条件下，三元杂交... 相似文献
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配种前营养策略对母猪妊娠早期胚胎成活率的影响 总被引：1，自引：0，他引：1
提高母猪妊娠早期胚胎成活率可以增加产仔数，提高经济效益。配种前营养对妊娠早期胚胎成活率具有重要的影响．这种影响的途径与卵泡发育和卵母细胞质量密切相关。本文综述了配种前营养．包括营养水平、能量来源、蛋白质、纤维等对后备母猪和哺乳母猪妊娠早期胚胎成活率的影响。 相似文献
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《中国兽医杂志》2016,(2)
为了解孕酮免疫生物传感器的临床应用效果,本试验对7头妊娠奶牛,8头产后发情奶牛和18头乏情奶牛的血浆雌二醇、促黄体素、促卵泡素和孕酮浓度进行了检测,同时应用的孕酮免疫生物传感器进行了乳汁孕酮检测。结果显示,孕酮免疫生物传感器检测的乳汁孕酮水平与ELISA检测血浆孕酮含量之间呈正相关。该优化的生物传感器检测奶牛妊娠的孕酮浓度范围为10~15 ng/mL,发情的孕酮浓度范围为4~10 ng/mL;持久黄体的孕酮浓度范围为10~16 ng/mL,卵巢静止的孕酮浓度范围为3~7 ng/mL,黄体囊肿的孕酮浓度范围为12~18 ng/mL。表明该孕酮免疫生物传感器能有效的检测奶牛妊娠、发情和繁殖障碍疾病的乳汁孕酮水平。 相似文献
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１分析病因
１.1母猪营养不足,降低了激素分泌量,母猪饲料单一,在怀孕或哺乳期营养不促足,再加上产仔数多,哺乳期失重过多,造成母猪断奶时过瘦,抑制了下丘脑产生促性腺激素释放因子,降低了促黄体素和促卵泡素的分泌,推迟了经产母猪的再发情。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献