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1.
利用2b-RAD技术对119尾黄条[鱼师](Seriola lalandi)个体进行测序,共获得黄条[鱼师]SNP分子标记26665个,对黄条[鱼师]个体的体质量和全长这2个重要生长性状进行全基因组关联分析,筛选与体质量和全长性状相关的SNP位点和候选基因。结果显示,黄条[鱼师]体质量性状中共筛选到17个体质量显著关联的SNP位点,找到17个可能的候选基因,全长性状共筛选到12个潜在显著关联位点,找到12个可能的候选基因。利用KEGG数据库对可能的候选基因进行Pathway分析,得知候选基因主要参与了细胞或组织生长发育相关的代谢通路调控过程,可能是影响黄条[鱼师]生长性状密切相关的重要候选SNP位点和功能基因,结果可为今后黄条[鱼师]种质资源可持续利用和育种提供遗传信息资料积累。 相似文献
2.
利用2b-RAD技术对119尾黄条鰤(Seriola lalandi)个体进行测序,共获得黄条鰤SNP分子标记26665个,对黄条鰤个体的体质量和全长这2个重要生长性状进行全基因组关联分析,筛选与体质量和全长性状相关的SNP位点和候选基因。结果显示,黄条鰤体质量性状中共筛选到17个体质量显著关联的SNP位点,找到17个可能的候选基因,全长性状共筛选到12个潜在显著关联位点,找到12个可能的候选基因。利用KEGG数据库对可能的候选基因进行Pathway分析,得知候选基因主要参与了细胞或组织生长发育相关的代谢通路调控过程,可能是影响黄条鰤生长性状密切相关的重要候选SNP位点和功能基因,结果可为今后黄条鰤种质资源可持续利用和育种提供遗传信息资料积累。 相似文献
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4.
Chotichayapong C Wiengsamut K Chanthai S Sattayasai N Tamiya T Kanzawa N Tsuchiya T 《Fish physiology and biochemistry》2012,38(5):1533-1543
Myoglobin from Asian swamp eel Monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. The purified Mb of 0.68?mg/g wet weight of muscle was determined for its molecular mass by MALDI-TOF-MS to be 15,525.18?Da. Using isoelectric focusing technique, the purified Mb showed two derivatives with pI of 6.40 and 7.12. Six peptide fragments of this protein identified by LC-MS/MS were homologous to Mbs of sea raven Hemitripterus americanus, yellowfin tuna Thunnus albacores, blue marlin Makaira nigicans, common carp Cyprinus carpio, and goldfish Carassius auratus. According to the Mb denaturation, the swamp eel Mb had thermal stability higher than walking catfish Clarias batrachus Mb and striped catfish Pangasius hypophthalmus Mb, between 30 and 60?(°)C. For the thermal stability of Mb, the swamp eel Mb showed a biphasic behavior due to the O(2) dissociation and the heme orientation disorder, with the lowest increase in both Kd(f) and Kd(s). The thermal sensitivity of swamp eel Mb was lower than those of the other Mbs for both of fast and slow reaction stages. These results suggest that the swamp eel Mb globin structure is thermally stable, which is consistent with heat-tolerant behavior of the swamp eel particularly in drought habitat. 相似文献
5.
Hidemasa Kawakami Hirofumi Yamashita Masahiro Sakai 《Journal of the World Aquaculture Society》2000,31(2):213-217
The pathogenicity of Photobacterium damsela subsp. piscicida to yellowtail Seriola quinqueradiata and goldstriped amberjack S. aureovittata was investigated. The 10-d cumulative mortality of yellowtail and goldstriped amberjack challenged by 1.2 × 102 CFU/fish of P. damsela piscicida was 70% and 10%. respectively. Serum bactericidal activity of goldstriped amberjack was higher than that of yellowtail. Although the phagocytic activity against P. damsela piscicida was not observed in kidney leukocytes of yellowtail and goldstriped amberjack, the production of superoxide anion in goldstriped amberjack kidney leukocytes against P. damsela piscicida was higher than that of yellowtail. 相似文献
6.
The karyotype and chromosomal characteristics of the yellowtail kingfish Seriola lalandi lalandi were investigated by examining metaphase spreads from kidney cells of 4-month-old fish artificially bred using wild-captured broodstock from the coast of South Australia. A total of 80 mitotic metaphases from 10 individual fish were analysed, with 52 spreads having a mode of 2 n =48 chromosomes, representing 65% of the metaphases observed. The diploid consisted of two metacentric, two submetacentric, six subtelocentric and 38 telocentric chromosomes, with the fundamental number of chromosome arms being 52. The total haploid chromosome length was approximately 44.412 μm. This study shows that the karyotype of S. lalandi lalandi differs from those reported previously for other Seriola species in having one pair of metacentric chromosomes. 相似文献
7.
A total of 31 antibody-secreting hybridoma cells against the yellowtail ascites virus (YAV) were established. These monoclonal antibodies (MAbs) reacted with the cytoplasm of YAV-infected CHSE-214 cells, but not with uninfected CHSE-214 cells in an immunofluorescence test. Using these MAbs, two classes of polypeptides (VP2 and VP3) were characterized by immunoprecipitation followed by SDS-PAGE, although one MAb did not react with either polypeptide. Fifteen out of the 17 MAbs that were reactive with VP2 polypeptides neutralized virus infectivity, but all 13 MAbs that were reactive with VP3 did not neutralize infectivity. In the immunofluorescence test, 29 out of the 31 MAbs obtained showed the same reaction pattern to 12 YAV isolates from yellowtail, Seriola quinqueradiata, goldstriped amberjack, Seriola aureovittata, and threeline grunt, Parapristipoma trilineatum, from different geographical regions. The remaining two MAbs showed slightly different reaction patterns to the YAV isolates. The reaction patterns of the MAbs to the VR-299, Sp and Ab strains of IPNV were also investigated. Fourteen MAbs reacted to all three IPNV strains. The other 17 MAbs showed a negative reaction with at least one strain. 相似文献
8.
Pseudotuberculosis is a bacterial septicaemia caused by Photobacterium damselae subsp. piscicida in several marine fish species. Yellowtail Seriola quinqueradiata is the most sensitive fish species to this disease. The internal organs of naturally infected yellowtail exhibit whitish
spots, tubercle-like tissue structures, consisting of bacterial accumulations. There have been many trials for experimental
infection, however adequate method of infection that reproduces moderate mortality and primary clinical signs has not yet
established. Present investigation evaluated an immersion infection method by using logarithmic culture-phase bacteria resulting
in higher mortality than that using stationary culture-phase bacteria. Typical white spots on the spleen and kidney were also
observed constantly in dead fish. Transmission electron microscopy and fluorescent antibody microscopy showed bacterial clusters
not only in the spleen and kidney but also in the blood channels in the secondary gill filaments. These results were confirmed
repeatedly by plural experiments. The use of logarithmic-phase bacteria in immersion infection is an appropriate technique
to reproduce moderate mortality and primary clinical signs, which will be a reliable infection method also for the challenge
test of pseudotuberculosis vaccine. 相似文献
9.
ABSTRACT: The effect of taurine on growth of yellowtail juveniles Seriola quinqueradiata was investigated by a feeding experiment of diets containing various taurine levels. Test diets supplemented with 0, 0.5, 1.0, 1.5 and 2.0% of taurine were prepared. These diets were fed to yellowtail juveniles with an initial mean body weight of 0.5 g for 6 weeks. Supplementation of taurine in the diet of yellowtail improved their growth performance significantly ( P < 0.05) over the initial 3-week period. The fish fed with the taurine-supplemented diet improved in percent gain and feed efficiency over both 3 and 6 weeks. Taurine content in the muscle proportionally increased with the dietary taurine level. The fish fed without supplemented taurine diet showed higher contents of serine in the muscle. With each increase in the inclusion level of taurine content in the diet, the concentration of serine in the muscle decreased. The cystathionine content in the muscle of each group was unchanged. These results suggest that taurine supplementation in the diet not only improves growth but also affects the sulfur amino acid metabolism of yellowtail juveniles. 相似文献
10.
Abstract. The pathogenicity of the agent causing viral nervous necrosis (VNN) of striped jack, Pseudocaranx dentex (Bloch & Schneider), was examined in striped jack and other selected marine fish species. Fish were exposed to purified striped jack nervous necrosis virus (SJNNV) (0·1–100 ng ml−1 ) or homogenates of diseased striped jack larvae. Striped jack larvae (3·5 and 4·4 mm total length) were susceptible to the virus, but juveniles (78 mm) were not. The viral antigens were detected by indirect ELISA and the characteristic pathological changes, i.e. vacuolation in the retina and brain, were reproduced in the affected larvae. The infection was also established in healthy larvae by cohabitation with the diseased larvae. Larvae of red sea bream, Pagrus major Temminck & Schlegel, yellowtail, Seriola quinqueradiata Temminck & Schlegel, and goldstriped amberjack, Seriola lalandi Valenciennes, were not susceptible to SJNNV. 相似文献
11.
Junpei Shinagawa Haruka Morino Toshiro Masumoto Haruhisa Fukada 《Fisheries Science》2017,83(4):607-617
Because a diet rich in docosahexaenoic acid (DHA) is beneficial to human health, it is desirable for yellowtail Seriola quinqueradiata intended for human consumption to contain a high amount of DHA. We investigated the effects on the growth and fatty acid composition of yellowtail of replacing some of the pollack liver oil (PO) in the yellowtail diet with a DHA-rich tuna oil (TO). Experimental diets were prepared in which PO was replaced with 0, 15, 30, and 60% TO. Replacement of PO with TO did not negatively affect growth in a 16-week trial. In contrast, the feed efficiency of the group that received 60% TO was significantly higher than that of the control group after 6 weeks. The DHA level in the whole fish body increased with increasing dietary TO level. The groups that received 30 and 60% TO replacement diets showed significantly higher DHA levels than those of the control group. DHA level in the fillet was significantly higher in the group that received 60% TO than in the control group. DHA retention tended to increase with increasing TO level. These results show that utilizing TO as a fat source can increase DHA accumulation without incurring any adverse effects on the growth of yellowtail. 相似文献
12.
Itaru Shioya Koji Inoue Akihisa Abe Akira Takeshita Takahiro Yamaguchi 《Fisheries Science》2011,77(5):883-889
The meat quality of farmed yellowtail Seriola quinqueradiata fed on extruded pellets (EP) containing 0.5% (v/v) red pepper (experimental group) was compared with yellowtail of the same
age fed on EP (control group). In 1-year-old yellowtail, the crude lipid content of the dorsal muscle of the experimental
group tended to be lower than that of the control group. In contrast, there was no difference in the lipid content of the
dorsal muscle between the control group and the experimental group in 2-year-old yellowtail. The muscle texture of the experimental
group was significantly firmer than that of the control group, with the effect of red pepper unrelated to fish age and lipid
content. Color change of red muscle of the experimental group was significantly lower than that of the control group, and
the content of thiobarbituric acid-reactive substances in the red muscle was significantly lower in the experimental group
than in the control group. These results are the first to demonstrate that the inclusion of red pepper in the diet is able
to reduce the loss of muscle texture firmness and to slow down color change in red muscle of yellowtail. 相似文献
13.
SHINJI UEHARA CHRISTOPHER T. TAGGART TAKUMI MITANI IAIN M. SUTHERS 《Fisheries Oceanography》2006,15(5):351-362
We assess the effect of drifting seaweed (Sargassum sp.) biomass, geography and hydrography on juvenile yellowtail (Seriola quinqueradiata) abundance variation off the southeast coast of Japan, near the Kuroshio Current. The amount of drifting seaweed mats progressively increased northeastward into the cooler, coastal waters. Frontal structure indexed using a station‐to‐station ΔSST did not explain spatial variation in the seaweed mat distribution, although the western extent of the Kuroshio Current appeared to act as a boundary. Juvenile yellowtail constituted 51–62% of the fish collected in association with drifting seaweed mats in April 1996 and 1997 and 29% in June 1996. The abundance of juvenile yellowtail was positively correlated with seaweed biomass. The geographic distribution of juvenile yellowtail associated with drifting mats varied among sampling periods, being more southwesterly in April and more northeasterly in June. Simple multiple regression models based on seaweed biomass and geographic distribution (latitude) explained between 35% and 43% of the variation in juvenile yellowtail abundance in spring. Associations with spatial and temporal variations in hydrographic conditions did not contribute to explained variation in a meaningful manner. The results presented here indicate that, off the southeast coast of Japan during April, yellowtail juveniles are likely to be most abundant when seaweed biomass is high, occur offshore, and are bounded by the western extent of the Kuroshio Current near the 19–20°C SST isotherm. 相似文献
14.
Noriko Hosomi Takahiro Furutani Noriyuki Takahashi Toshiro Masumoto Haruhisa Fukada 《Fisheries Science》2014,80(3):483-492
Neuropeptide Y (NPY) and cholecystokinin (CCK) play important roles in regulating appetite in vertebrates, including mammals and fish. Understanding the appetite mechanism is important in aquaculture to improve production performance. The yellowtail Seriola quinqueradiata is one of the most cultured fish in Japan, but little is known about its appetite hormones. In the present study, complementary DNA encoding for NPY was cloned in yellowtail and consists of 604 bp, in which deduced amino acid sequences show high identity to those of other teleosts. In tissue distribution, the npy and cck mRNAs were detected in all examined tissues (whole brain, telencephalon, optic tectum, hypothalamus, cerebellum, pituitary, retina, stomach, pyloric caeca, anterior intestine, liver, and kidney). In the fasting experiment, only npy mRNA expression in the hypothalamus responded to fasting, showing a significantly high value compared with that in control fish. The expression of cck mRNA in the examined tissues did not change with fasting. The npy mRNA expression in the hypothalamus might be involved in feeding regulation in yellowtail. 相似文献
15.
A dihydropteroate synthase gene from the chromosomal DNA of the fish-pathogenic bacteria Lactococcus garvieae (formerly Enterococcus seriolicida ) was cloned. This gene was then chosen as the target for polymerase chain reaction (PCR). The designated PCR primer set only amplified a 709-bp DNA fragment from L. garvieae strains, and did not amplify the same molecular size fragment from related species of L. lactis, Enterococcus faecalis, E. faecium or β-haemolytic Streptococcus sp. The kidney tissue of yellowtail, Seriola quinqueradiata (Temminck and Schlegel), a species which is naturally infected with L. garvieae , and also kidney tissue samples of healthy yellowtail were stored in TNES-Urea. The DNA was extracted from tissue samples by a modification of the standard method and by a boiled-extraction method. In particular, template DNA was utilized within 30 min following extraction and purification by the boiled-extraction method. These species-specific PCR primers could amplify a L. garvieae target sequence from yellowtail which were naturally infected with L. garvieae . The total procedure for the diagnosis of L. garvieae infections in fish, from the point of DNA extraction to observation in an agarose gel following electrophoresis, can be performed in less than 4 h. 相似文献
16.
Fukada Haruhisa Kitagima Renato Shinagawa Junpei Morino Haruka Masumoto Toshiro 《Fisheries Science》2020,86(1):107-118
Docosahexaenoic acid (DHA) is an essential fatty acid for marine carnivorous fish. Algal meal (AM), available as a new dietary DHA source, could completely replace dietary fish oil (FO). In this study, dietary FO was replaced with plant oil mixtures and AM in juvenile yellowtail Seriola quinqueradiata to investigate its effects on growth performance and fatty acid composition. The FO control diet was prepared with only pollack liver oil as the lipid source. For the non-FO diets, pollack liver oil was completely replaced with mixtures of canola oil and palm oil, with AM supplementation at 0% (AM0), 1% (AM1), 2% (AM2), 3% (AM3), and 4% (AM4). After completion of the 8-week feeding trial, the AM2 group showed significantly higher values for final body weight and feed efficiency than the AM0 group. No significant differences were observed in the other parameters of growth performance. Whole-body fatty acid composition reflected the dietary fatty acid composition in all dietary groups. These findings demonstrate that AM is useful as a DHA source in yellowtail aquaculture, thus contributing to a reduction in the use of FO in fish diets.
相似文献17.
Attempts have been made to elucidate the denaturation profiles of tuna myoglobin (Mb) in comparison with horse Mb. Intensive absorbance characterization was carried out for derivatives (deoxy, oxy, met forms) of Mb. The wavelength of the maximum absorbance of tuna Mb was shorter only by 1 nm for deoxy and metMb, while it was 4 nm shorter for the second peak of metMb. Percentage Mb denaturation (PMD) was measured under a combination of pH (5.6, 6.5, 7.4) and temperature (70, 75, 80 °C). Tuna Mb was almost completely denatured even during the initial incubation at 75 and 80 °C at all pHs examined. During the incubation at 70 °C, the PMD values for tuna Mb were 88.5, 52.1, and 67.7% at pH 5.6, 6.5, and 7.4, respectively. The denaturation of tuna Mb proceeded even at 55 °C, but denaturation rates were very slow at pH 6.5. On the other hand, horse Mb was found to be very stable at pH 6.5 and 7.4 at all temperatures examined, except at pH 6.5 and 80 °C. At pH 5.6, the PMD values of horse Mb gradually increased, especially at 75 and 80 °C. These different Mbs showed quite different denaturation profiles. 相似文献
18.
Kentaro Higuchi Yutaka Takeuchi Misako Miwa Yoji Yamamoto Kazunobu Tsunemoto Goro Yoshizaki 《Fisheries Science》2011,77(1):69-77
Recently, we developed an intraspecies spermatogonial transplantation technique in a pelagic egg spawning marine teleost,
nibe croaker Nibea mitsukurii. Nibe croaker is an ideal candidate recipient for spermatogonial transplantation since it has a short generation time and
small body size. In the present study, yellowtail Seriola quinqueradiata spermatogonia were transplanted into nibe croaker larvae, and the behavior of transplanted spermatogonia in recipient gonads
was observed. Three weeks post-transplantation, yellowtail spermatogonia were incorporated into the gonads of 72 out of 88
recipients. An antiproliferating cell nuclear antigen was detected in incorporated yellowtail spermatogonia, suggesting that
the xenogenic germ cells were proliferating in recipient gonads. Yellowtail vasa-positive spermatogonia survived for 11 months after transplantation in the gonads of recipient fish. Thus, we showed that
the microenvironment in nibe croaker gonads can support the colonization, proliferation, and survival of germ cells derived
from a different taxonomic family. 相似文献
19.
Kenji Takii Kohji Kawamura Motoji Nakamura Hidemi Kumai Yasuko Yoshizawa 《Fisheries Science》2001,67(1):52-57
SUMMARY: An inhibitory effect of Kunitz and Bowman-Birk soybean trypsin inhibitors (K- and BB-SBTI) was investigated on tryptic and chymotryptic proteinases of a brood of tiger puffer Takifugu rubripes and yellowtail Seriola quinqueradiata . These assist to comprehend the differences in dietary soybean meal (SBM) utility by fish species and to develop low cost SBM diets with low contents of fish meal. The K-SBTI strongly inhibited tryptic proteinase and weakly inhibited chymotryptic proteinase from the hepatopancreas and intestine including pyloric ceca of the fishes. The BB-SBTI strongly inhibited both proteinases of the fishes. The K-SBTI inhibition for tryptic protease and the BB-SBTI inhibition for tryptic and chymotryptic proteases were relatively constant in tiger puffer with growth of 12–199 g bodyweight. In yellowtail with growth of 7–672 g bodyweight, the K-SBTI inhibition for tryptic protease and the BB-SBTI inhibition for chymotryptic protease were also relatively constant. The BB-SBTI inhibition for yellowtail tryptic protease typically fell in the stage from 7 g to 57 g bodyweight, and then decreased slightly or was maintained constantly. These results indicate that there are different inhibitory modes of K- and BB-SBTI and there is inter- and intraspecific diversity of tryptic protease conformation between the two species. 相似文献
20.
Lancefield group C Streptococcus dysgalactiae infection responsible for fish mortalities in Japan 总被引：2，自引：0，他引：2
Nomoto R Munasinghe LI Jin DH Shimahara Y Yasuda H Nakamura A Misawa N Itami T Yoshida T 《Journal of fish diseases》2004,27(12):679-686
A Lancefield serological group C Streptococcus sp. was isolated from cultured amberjack, Seriola dumerili Risso, and yellowtail, Seriola quinqueradiata Temminck and Schlegel, immunized with Lactococcus garvieae commercial vaccines in Japan. The isolated bacteria were Gram-positive cocci, auto-aggregating in saline, morphologically long chains in growth medium, catalase negative and alpha-haemolytic on blood agar. An almost complete gene sequence of the 16S rDNA of two isolates was determined and compared with that of bacterial strains in the database. The isolates were identified as Streptococcus dysgalactiae based on the results of the 16S rDNA sequence, the bacteriological properties and the Lancefield serological grouping. Oligonucleotide primers specifically designed for the 16S-23S rDNA intergenic spacer region of S. dysgalactiae amplified a gene from all the fish isolates, as well as the type strains alpha-haemolytic S. dysgalactiae subsp. dysgalactiae ATCC430738 and beta-haemolytic S. dysgalactiae subsp. equisimilis ATCC35666, but not those of S. equi ATCC33398, Lactococcus garvieae ATCC43921 and L. garvieae KG9408. The severe necrotic lesions of the caudal peduncle seen in experimentally infected fish were similar to those seen in naturally infected fish. 相似文献