共查询到20条相似文献，搜索用时 640 毫秒
1.
2.
旨在研究激光结合丹参对Ⅱ型胶原诱导类风湿性关节炎大鼠模型的类风湿性因子、免疫指标、脏器系数及炎症因子的变化情况,为治疗类风湿性关节炎提供参考依据。用鸡Ⅱ型胶原诱导健康雄性Wistar大鼠为类风湿性关节炎大鼠,造模成功后,随机分为5组,于第35天处死大鼠,取大鼠各脏器,称取其湿重并计算脏器系数,检测并比较各处理组大鼠血清中类风湿性因子(RF)、IL-6、IL-1水平及免疫球蛋白水平等。处理组大鼠血清中的免疫指标IgA水平丹参结合激光组高于模型组及其他治疗组(P0.05),免疫指标IgG和IgM水平丹参结合激光组低于模型组及其他治疗组(P0.05)。与模型组、空白对照组比较,各处理组大鼠脏器系数含量降低(P0.05)。各处理组大鼠血清中IL-1、IL-6及RF含量均低于模型组且高于对照组(P0.05)。可见激光结合丹参对Ⅱ型胶原诱导类风湿性关节炎大鼠模型具有一定程度的治疗效果。 相似文献
3.
4.
犬瘟热病毒H、F、N基因试验免疫研究 总被引：6，自引：1，他引：6
以构建的真核表达载体pVAXLPH、pVAXLPF和pVAXLPN为基因疫苗,分组进行了免疫小鼠试验,对免疫鼠体内免疫应答水平进行了检测。结果显示:所构建的基因疫苗可以诱导小鼠产生特异性免疫应答反应,pVAXLPH pVAXLPF pVAXLPN 3个基因混合免疫小鼠诱导产生了较强的体液免疫和细胞免疫应答,免疫3次后血清中抗CDV ELISA抗体效价为0.332,而免疫前为0.158;抗CDV中和抗体效价为1∶(4~16);CD4 T/CD8 T比值为2.05±0.38,而对照组为1.99±0.56;免疫小鼠脾细胞对CDVLP及ConA刺激的增殖反应值分别为0.384和0.356。基因疫苗免疫犬试验中,进行了单用基因疫苗免疫和基因疫苗与CDV弱毒疫苗联合免疫效果的比较研究。结果显示,基因疫苗免疫3次后,血清中抗CDV ELISA抗体效价为0.296,抗CDV中和抗体效价为1∶(8~32)。基因疫苗免疫2次,再使用弱毒疫苗加强免疫1次,血清中抗CDV ELISA抗体效价为0.433,抗CDV中和抗体效价为1∶(16~64)。 相似文献
5.
通过自身抗原 Sm B/ B′主要表位进行基因免疫 ,诱导系统性红斑狼疮 ( SL E)样综合征的动物模型 ,为进一步探讨 SL E的发病机理奠定基础。以 RT-PCR的方法获得 Sm B/ B′主要表位编码基因 ,构建表达载体 pc DNA3 -Sm B/ B′,基因免疫后以免疫印迹法检测抗 Sm抗体、EL ISA检测抗 ds DNA抗体、免疫荧光法检测肾脏损伤。结果显示基因免疫后小鼠均产生抗 Sm抗体、抗 ds DNA抗体 ,且能诱发动物肾小球 Ig G类免疫复合物的沉积 ,表明自身抗原表位的基因免疫能成功诱导 SL E样综合征小鼠模型。 相似文献
6.
《黑龙江畜牧兽医》2014,(13)
为了研究鸡CD40L作为分子免疫佐剂的免疫原性,试验采用鸡的脾脏细胞克隆鸡CD40L基因,构建表达载体pET28a-cCD40L进行原核表达,以纯化的重组蛋白免疫长耳白兔,获得兔抗鸡CD40L多克隆抗体,用间接ELISA检测多克隆抗体效价,并进行Western-blot检测。结果表明:获得分子质量为26 ku的重组融合蛋白,多克隆抗体效价为1∶12 800;表达的融合蛋白不仅能与鼠抗His克隆抗体发生特异性反应,而且与获得的兔抗鸡CD40L多克隆抗体发生特异性反应。说明鸡CD40L融合蛋白具有较高的反应活性,所获得的兔抗鸡CD40L多克隆抗体具有良好的特异性。 相似文献
7.
本试验旨在探讨一种能够快速、简便及特异检测MHV病毒的金标检测方法。采用柠檬酸三钠还原法制备胶体金颗粒,标记抗小鼠肝炎病毒(MHV)抗体,选择优化层析条件,建立双抗体夹心模式的免疫层析法试纸条对MHV进行检测。20 nm左右的胶体金颗粒、5.05 μg为最低稳定抗体量(稳定0.5 mL胶体金)、M135层析材料、3%BSA是最佳封闭液。640 μg/mL抗体蛋白为最适检测线包被浓度,而800 μg/mL抗体蛋白为质控线最适包被浓度。此试纸条能10 min快速特异地检测出MHV抗原,阳性时检测线和质控线均显示红色,阴性时则只有质控线显示红色。结果表明,胶体金免疫层析方法能快速、特异、稳定地检测MHV抗原。 相似文献
8.
研究He-Ne激光结合丹参对类风湿关节炎大鼠的治疗效果,以期为临床风湿病治疗提供参考依据。将40只健康雄性Wistar大鼠随机分为5组,分别是A空白对照组、B模型组、C激光治疗组、D丹参治疗组和E丹参结合激光治疗组。除A空白对照组外,通过对其余各组大鼠注射鸡Ⅱ型胶原诱导后,成功建立了大鼠类风湿关节炎模型,分别给予对应的治疗,并分析各处理组大鼠大体情况、关节的肿胀度、评分及足踝关节X光片。研究表明:(1)与对照组相比,各处理组的大鼠类风湿关节炎评分、脚掌厚度均不同程度升高且有显著差异,其中模型组的最高,丹参结合激光治疗组最低,其余两组均降低且无显著差异;(2)X片显示:模型组大鼠关节出现了严重的骨质吸收、溶解、关节面融合,关节间隙变窄,关节基本损毁;丹参结合激光组可显著保留关节结构并使其免受侵蚀,而其余两组效果比丹参结合激光组稍弱一些。(3)与对照组相比,各处理组类风湿因子、血管内皮细胞生长因子及肿瘤坏死因子均升高,且有显著差异;与模型组相比,各药物组大鼠类风湿因子、血管内皮细胞生长因子及肿瘤坏死因子均有所降低,且均具有显著差异(P0.05),其中下降最明显的是He-Ne激光结合丹参治疗组。表明He-Ne激光结合丹参,能有效控制和改善类风湿关节炎大鼠的病情发展,具有良好的治疗效果。 相似文献
9.
利用本实验室已诱导并纯化后的重组毒素蛋白制成类毒素免疫BALB/c小鼠,提取小鼠脾脏mRNA。通过RT-PCR和SOE-PCR获得由抗体重链可变区(VH)-Linker-轻链可变区(VL)构成的750bp左右单链抗体(ScFv)基因片段。随后利用噬菌体展示技术成功构建了抗产气荚膜梭菌ε毒素噬菌体单链抗体(Phage-ScFv)库。并通过"吸附-洗脱-扩增",5轮高效的淘洗筛选系统,最终筛选出特异性强的噬菌体抗体。最后,对强阳性的单链抗体侵染E.coli HB2151进行表达,SDS-PAGE结果显示其相对分子质量为30 000左右,与预期结果一致,并且能以分泌形式表达。本试验为抗产气荚膜梭菌ε毒素中毒治疗及其分子致病机制的研究奠定了基础。 相似文献
10.
为了探究重链抗体在骆驼免疫保护中的生物学作用,本研究利用Protein G和Protein A亲和层析纯化新疆双峰驼血清中总IgG和重链抗体IgG2,并免疫昆明小白鼠制备抗双峰驼总IgG和抗双峰驼重链抗体IgG2的多抗血清;通过ELISA检测双峰驼在体液免疫应答过程中针对spaA-N、溶菌酶和蒜氨酸酶3种抗原的重链抗体的滴度变化。结果从新疆双峰驼血清中亲和层析纯化出天然重链抗体IgG2,蛋白质分子质量约为46 ku;免疫昆明小白鼠后获得抗双峰驼总IgG多抗血清的效价为1∶204800,抗双峰驼重链抗体IgG2多抗血清的效价为1∶6400。在spaA-N免疫骆驼诱导的体液免疫应答过程中,重链抗体IgG2出现延迟反应。3种蛋白均能诱导抗原特异的IgG2亚类重链抗体的产生。 相似文献
11.
12.
13.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
16.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
18.
19.
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献