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显微注射Ca^2＋激活小鼠M Ⅱ期卵母细胞 总被引：2，自引：0，他引：2
胞质内显微注入Ｃａ＾２＋可以激活小鼠Ｍ Ⅱ期卵母细胞，且随着卵龄的增加，激活率明显升高。卵龄小于１６ｈ（ｈＣＧ注射后）注射Ｃａ＾２＋引起的激活率很低，卵龄超过１８ｈ，激活率达到５０％以上。卵龄小于１６ｈ的卵母细胞，卵内注入超纯水未能激活卵母细胞，而在１８￣１９ｈ卵龄组，注射水的对照组激活率为３５％，但显著或极显著地低于注射Ｃａ＾２＋的试验组。激活前注射ＥＤＴＡ卵母细胞的激活率约为３０％，激活后注射 相似文献
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脉冲次数和卵龄对小鼠卵母细胞电活化效果的影响 总被引：2，自引：0，他引：2
以一定电场条件、不同次数的电脉冲刺激注射ＨＣＧ后不同时间（１５、１７、１９、２１和２５ｈ）小鼠卵母细胞，研究了不同电场条件下脉冲次数和卵龄对卵母细胞活化效果的影响。 相似文献
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将卵母细胞透明带溶孔、透明带切口、将精子植入卵周隙内以及将精子注射到卵母细胞质内的辅助受精技术(assisted fertilizati-on),可为精子排除透明带障碍或越过透明带和精一卵细胞膜融合等过程,提高精液利用率和治疗某些类型的不孕症。前三种技术可排除透明带对精子形成的障碍,这时的精子应是能运动的而且能与细胞质膜融合。第四种技术可排除透明带和精卵膜融合这两重障碍,精子不一定是能运动的、活的、甚至完整的。这些技术可以提高家畜和野 相似文献
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采用鞘内注射降钙素基因相关肽（ＣＧＲＰ）受体拮抗剂人ＣＧＲＰ片段８－３７（ｈＣＧＲＰ８－３７），探讨了佐剂性关节炎大鼠初级感觉系统中ＣＧＲＰ增多的意义。结果：佐剂前注射ｈＣＧＲＰ８－３７的动物，在注射佐剂后１～２０ｄ，患肢及健肢皮温均高于对照组（Ｐ＜０．０５）；患肢足容积明显大于对照组（Ｐ＜０．０５），并且在形成变态反应性佐剂性关节炎时（佐剂后１３～２０ｄ），健肢足容积也明显增加（Ｐ＜０．０５）；但双后肢的痛阈变化与对照组相比没有明显差异（Ｐ＞０．０５）。上述结果表明，佐剂前鞘内注射ｈＣＧＲＰ８－３７，使急性关节炎和多发性关节炎均有所加重。佐剂后１２ｄ注射ｈＣ－ＧＲＰ８－３７的动物，双后肢的肿胀积分均明显高于对照组（佐剂后１８～５０ｄ，Ｐ＜０．０５或０．０１），痛级别也明显高于对照组（佐剂后１４～５０ｄ，Ｐ＜０．０１），但痛阈的变化不明显（Ｐ＞０．０５）。因此佐剂后１２ｄ鞘内注射ＣＧＲＰ的拮抗剂也可加重关节炎的炎症反应。这进一步证明了脊髓ＣＧＲＰ可能具有抗炎和抑制免疫反应的作用。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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Reasons for performing study: Clinical experiences indicate that centesis of the lateral compartment of the femorotibial joint is often unsuccessful. Objective: To determine the accuracy of 2 published and 2 unpublished techniques of centesis of the lateral compartment of the femorotibial joint. Hypothesis: It was hypothesised that a drug can be administered into the lateral compartment of the femorotibial joint via a diverticulum of this joint that surrounds the medial aspect of the long digital extensor tendon and that this technique is more accurate than described techniques of centesis of this compartment. Methods: Twenty‐four stifles of 12 horses were divided equally into 4 groups and a radiocontrast medium injected into the lateral compartment of the femorotibial joint of each group using a hypodermic needle inserted: 1) caudal to the lateral patellar ligament and proximal to the tibial plateau, 2) caudal to the long digital extensor tendon and proximal to the tibial plateau, 3) between the long digital extensor tendon and bone of the extensor groove of the tibia or 4) directly through the long digital extensor tendon until it contacted bone. Twelve veterinary students who had no experience using any of these techniques performed the injections. Accuracy of each technique was determined by examining radiographs obtained after injecting the contrast medium. Results: The most successful technique for arthrocentesis was by inserting a needle through the long digital extensor tendon. This approach was successful in all attempted cases, whilst other techniques had lower rates of success. Conclusions: The lateral compartment of the femorotibial joint can be accessed accurately by inserting a needle through the long digital extensor tendon as it lies within the extensor groove. Other techniques may not be as accurate for clinicians inexperienced in arthrocentesis of the lateral compartment of the femorotibial joint. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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Marioni-Henry K Vite CH Newton AL Van Winkle TJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):851-858
A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified. 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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根据JJF1059-1999《测量不确定度评定与表示》建立了高效液相色谱法测定鸡肉中土霉素残留测量的不确定度评定方法,评定过程包括建立测量程序、分析不确定度来源、对不确定度分量进行量化。将不确定度归类为两大类。随机效应导致的不确定度可以通过一系列具有统计意义的重复观测值进行A类评定;系统效应导致的不确定度通过各测量器具、设备的说明书、校准证书提供的参数进行B类评定。本方法鸡肉中土霉素残留量的相对合成标准不确定度约为3.9%。 相似文献
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以5个金柑属品种、枳壳、软枝酸橘及广西土柠檬实生苗为材料,进行复水成活率、旱害指数、叶片相对含水量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、丙二醛（MDA）、脯氨酸等生理指标及隶属函数的综合分析,为柑橘抗性砧木品种选育提供参考。结果表明：胁迫20d复水后的成活率融安滑皮金柑和长寿金柑最高,为100%,软枝酸橘最低,为88.9%;随着干旱胁迫加剧,各品种的旱害指数呈上升趋势,长寿金柑的旱害指数最低,为0.25,软枝酸橘的旱害指数最高,为0.61;从叶片相对含水量的结果来看,长寿金柑的变化幅度最小,软枝酸橘的变化幅度最大,在第18d时分别为61.19%和37.85%;3种保护酶之间相互协同作用减轻植株受害程度,其活性变化幅度与丙二醛和脯氨酸的含量变化相似。利用隶属函数法综合分析,得出各品种的抗旱性强弱依次为长寿金柑>枳壳>融安滑皮金柑>融安金柑>山金柑>广西土柠檬>金枣>软枝酸橘。 相似文献
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分别用CIDR（孕酮阴道栓）＋PMSG（处理1）和CIDR＋PMSG＋LH（处理2）两种处理方法对山羊进行同期发情处理。结果发现,两种处理均以撤栓后第1d发情率最高。撒栓后0-24h和24h-48h的发情率,处理1和处理2（61．4％,13．6％和48％,20％）之间差异不显著;撤栓后48h-72h的发情率,处理1（4．59,6）显著低于处理2（24％）（P〈0．05）;撤栓后0～72h发情率,两种处理（79．5％,92％）无显著差异（P〉0．05）。从手术时对卵巢的观察可以看出,有大卵泡或排卵的发情羊,处理1为91．2％,处理2为100％,二者无显著差异;排卵的发情羊,处理1为（58．8％）显著低于处理2为（91．2％）（P〈0．05）。 相似文献