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1.
首次对比研究了塞曼效应石墨炉原子吸收法测定生物样品中钼含量。以硝酸钯 (Pd(NO3 ) 2 )、三氯醋酸 (TCA)、及Pd(NO3 ) 2 TCA作为基体改进剂可使吸光度分别增加 5倍、12 .4倍及 19倍 ,检出下限分别为 2 5ng/mL、12ng/mL、10ng/mL。以塞曼效应扣除背景吸收 ,能有效地克服Fe、Cu、Mn等共存离子的干扰。通过对奶牛、雏鸡血清及组织中钼含量的测定可知 ,混合型的基体改进剂能够提高钼含量测定的灵敏度及回收率  相似文献   

2.
本文研究用石墨炉原子吸收分光光谱仪测定饲料原料及成品中硒含量的方法。用Pd(NO_3)_2作为基体改进剂,重点研究了基体改进剂的体积、灰化温度和原子化温度对吸光值的影响,同时以此为基础建立了饲料中硒的优化分析方法。此方法中硒含量在0~100μg/L成线性关系,检测限为0.33μg/kg,回收率为95.0%~105.3%。该方法具有简单、灵敏、准确的优点,适合饲料原料和成品中硒的含量分析。  相似文献   

3.
采用水浴湿法消化 ,以 Pd( NO3) 2 - Triton X- 10 0作为基体改进剂 ,应用 Zeem an石墨炉原子吸收法测定了肝组织硒含量。试验结果表明 ,肝组织经水浴湿法消化测得的硒含量与消化炉湿法消化测定结果基本一致 ( P >0 .0 5) ,相对标准偏差为 1.11%,回收率为 95.80 %~ 10 0 .4 0 %。石墨炉原子吸收法测定硒的检测限为 5.0 μg/ L  相似文献   

4.
采用微波制样技术对样品进行消解,减少了消化过程中硒的损失,利用石墨炉原子吸收光谱法对醋酸地塞米松中微量硒进行测定.以4 mg/mL Pd(NO3)2作为基体改进剂,灰化温度为950 ℃,原子化温度为2 100 ℃时,经氘灯校正背景,测定硒含量,回收率在89.24%~112.37% 之间,方法的相对标准偏差为7.42%,检出限为0.53 ng/mL.该法具有简便、快速、灵敏、稳定、准确等优点,适用于醋酸地塞米松中微量硒的分析测定.  相似文献   

5.
采用微波消解技术处理样品,用石墨炉原子吸收光谱法测定,对消解溶剂和微波条件进行了优化,研究了石墨炉原子吸收法的测定条件。结果表明,以HNO3+HClO4+H2O2作为微波消解溶剂效果最佳,在基体改进剂NH4H2PO4和Pd(NO3)2存在下,可有效地消除基体的影响,建立了微波-石墨炉原子吸收光谱法测定鱼粉中镉(Cd)和铬(Cr)的方法,Cd和Cr检出限分别为0.17ng/mL和0.46ng/mL;回收率分别为88.2%~94.1%(RSD:2.7%,n=6)、91.7%~106.2%(RSD:6.6%,n=6)。  相似文献   

6.
畜禽的生长发育对硒的需求必不可少,但过量摄入也将引起畜禽中毒,因此在饲料及饲料原料对硒进行检测控制其含量是非常必要的。本文根据实际对饲料、饲料原料硒的测定,通过实验中加入不同的基体改进剂,以降低硒在灰化过程中的损失,以提高对硒测定准确度。  相似文献   

7.
文章深入研究了氢化物发生-原子荧光光谱法测定鹿饲料中硒的分析方法。研究结果表明,采用硝酸-高氯酸(8+1)混合酸消解样品,以2%盐酸介质作为载流,样品酸度控制在20%时进行样品分析,测定结果准确可靠。该方法的线性范围0~10μg/l,相关系数优于0.999,检出限0.07μg/l,加标回收率85.3%~116.9%。此方法具有简单易行、精密度好、回收率高等优点,适用于饲料中硒含量的测定。  相似文献   

8.
试验采用高效液相色谱法测定饲料样品中色氨酸的含量。样品经过处理后,用Agilent C18(4.6 mm×150 mm,5μm)色谱柱分离样品,以0.008 5 mol/l的乙酸钠+甲醇=95+5为流动相;检测波长280 nm,流动相流速1.0 ml/min。结果表明,色氨酸在4.656~298μg/ml范围内,峰面积与其浓度线性关系良好(r=0.999 9),最低检测限为0.033 5μg/ml,两种饲料中色氨酸含量均在国标允许的误差范围内。方法准确、简便、快速,适用于色氨酸的含量测定。  相似文献   

9.
96只"白来航"蛋鸡用于研究富含ω-3脂肪酸和天然抗氧化剂的配方饲料对蛋鸡蛋黄成分的影响。试验蛋鸡被随机分为4组:对照组(少量添加剂含量);FSE功能饲料组(150g亚麻籽+200mg维生素E+3g螺旋藻/kg饲料);FOSe功能饲料组(20g鱼油+0.2mg有机硒+3g螺旋藻/kg饲料)和FSE+FOSe功能饲料组(75g亚麻籽+10g鱼油+100mg维生素E+0.1mg有机硒+3g螺旋藻/kg饲料)。试验结果表明,这三种功能饲料都能增加蛋黄类胡萝卜素(P0.01)和ω-3脂肪酸的含量,饱和脂肪酸比例则下降,蛋黄脂质中油酸含量没有显著变化。这三种功能饲料能显著降低蛋黄胆固醇含量(P0.01)。各处理组鸡蛋煮后有可比的嗅觉感受。日粮中补充的硒和维生素E可协同作用增加鸡蛋中ω-3脂肪酸的含量。  相似文献   

10.
20~700μg/ml的La(NO3)3处理的苜蓿种子吸水速率明显加快,膜的通透性增大,呼吸作用增强.其中,用500μg/ml La(NO3)3处理效果最佳,用1200~1500μg/ml La(NO3)3处理则抑制种子萌发.  相似文献   

11.
12.
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。  相似文献   

13.
采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。  相似文献   

14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。  相似文献   

15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.  相似文献   

16.
用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10~0.82μg/g。表明:蜂蜜应视为天然富硒营养品。  相似文献   

17.
乳酸杆菌益生作用机制的研究进展   总被引:2,自引:0,他引:2  
乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和 Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。  相似文献   

18.
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.  相似文献   

19.
为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …  相似文献   

20.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。  相似文献   

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