共查询到20条相似文献，搜索用时 359 毫秒
1.
为建立一种对临床沙门菌株进行快速血清分型的策略，本研究尝试将玻片凝集法和液相芯片法进行联合应用。首先利用玻片凝集法对467株动物源沙门菌进行鉴定，然后对不能直接利用玻片凝集法鉴定血清型的沙门菌采用液相芯片法进行补充鉴定。结果显示，利用玻片凝集法可以直接检测409株沙门菌的O抗原和H抗原，包含49种血清型；结合液相芯片法在1 d内完成了其余58株沙门菌的O抗原和H抗原单因子的鉴定，共涉及10种血清型。结果表明，玻片凝集法具有快速鉴定O抗原优势，液相芯片法具有快速鉴定H抗原优势，2种方法结合应用的策略可以快速、高效地实现沙门菌血清分型的目的，可作为沙门菌血清型快速鉴定方案并予以推广。 相似文献
2.
《广东畜牧兽医科技》2015,(1)
根据志贺氏菌属侵袭性质粒抗原H(ipa H)基因、沙门氏菌属特异性基因和小肠结肠炎耶尔森氏菌(ail)毒力基因,分别设计3对特异性引物,通过优化、调整PCR条件,建立了一种能同时检测食蟹猴3种致病菌的多重PCR检测方法。临床初步应用于检测137例食蟹猴肛拭子样品137份,检出志贺氏菌属阳性12株,沙门氏菌属阳性1株,小肠结肠炎耶尔森氏菌阳性1株,与传统细菌培养生化鉴定方法检测结果一致。本试验建立的多重PCR方法具有快速、准确、简便、灵敏、稳定性高等优点,具有较高的实际应用价值,为食蟹猴腹泻病原菌提供了新的快速检测方法。 相似文献
3.
广东屠猪肉样品中大肠杆菌耐药性与毒力特征的分析 总被引：2，自引：0，他引：2
为分析广东地区屠猪肉中大肠杆菌(E.coli)药物敏感菌株的血清型、毒力基因和系统进化背景,本研究从屠猪肉样品中分离出112株E.coli,采用玻片凝集法鉴定血清型,琼脂稀释法测定10种抗菌药的敏感性,PCR方法检测7种毒力相关基因,多重PCR方法进行系统进化背景判定。结果显示,112株E.coli中,定型菌株95株,分别属于15种血清型,其中O65、O131、O8和O158为优势血清型。几乎所有菌株对氟苯尼考、多西环素和四环素高度耐药,而对头孢曲松高度敏感,其中多重耐药菌株多数耐5种以上药物,常见的多重耐药表型是氟苯尼考/氯霉素/多西环素/四环素/氨苄西林。PCR鉴定结果表明,含有毒力基因的菌株中38%至少具有两个毒力基因,其中EAST1+Stx2e和hlyF+Stx2e比较常见。比较常见的毒力基因为Stx2e和EAST1,STb基因仅在一株菌中检测到。多重PCR鉴定结果显示,屠猪肉样品中E.coli主要分布为共生型的A组和B1组。本研究为大肠杆菌病的控制和合理使用抗生素提供实验依据。 相似文献
4.
为了查明引起海口市某进境实验动物隔离场3例恒河猴死亡的原因，本试验分别采集3只恒河猴的心脏、肝脏、脾脏、肺脏和淋巴结样本，进行细菌分离，通过细菌形态特征、显微镜镜检、生化鉴定、16S核糖体RNA(16S rRNA)序列分析对分离菌进行病原学鉴定；采用纸片扩散法(K-B法)测定分离菌的耐药表型；采用玻片凝集法测定分离菌的血清型；采用动物回归试验分析分离菌的致病性。结果显示，从3只恒河猴上各分离鉴定出1株大肠杆菌，命名为H01株(分离自脾脏)、H02株(分离自淋巴结)和H03株(分离自肝脏);3株大肠杆菌对12种常用抗菌药物产生不同程度耐药，并且都呈现多重耐药，其中对阿莫西林、庆大霉素、氯霉素、氟苯尼考和复方新诺明表现为耐药，仅对阿莫西林-克拉维酸钾、阿米卡星和多黏菌素B表现敏感；玻片凝集试验显示，H01株的血清型为O8,H02株的血清型为O3,H03株的血清型为O9;动物回归试验显示，3株分离菌都是强毒株。本试验为实验动物恒河猴源大肠杆菌病的防治提供参考依据。 相似文献
5.
本研究建立了鉴定猪胸膜肺炎放线杆菌(APP)血清型的多重PCR方法,并对鲁西地区流行的APP血清型进行了鉴定.根据猪胸膜肺炎放线杆菌的外膜脂蛋白(OmlA)基因设计1对种特异性引物;并且根据血清1型、5型、7型荚膜多(cps)基因设计型特异性引物,建立检测血清1型、5型、7型的PCR方法.运用多重PCR对临床分离鉴定的89株APP进行血清型鉴定,结果表明建立的多重PCR检测方法特异性和敏感性良好,可作为猪传染性胸膜肺炎快速诊断和流行病学调查的重要手段. 相似文献
6.
《中国动物传染病学报》2016,(3)
根据Gen Bank中猪链球菌(Streptococcus suis,Ss)2、7、9血清型的荚膜多糖基因簇中的斜基因序列,分别设计3对引物,在完成最佳条件筛选、特异性、敏感性试验的基础上,建立了一种快速区分猪链球菌2、7、9三种血清型的多重PCR检测方法,并对我国广东省分离鉴定的猪链球菌进行分型鉴定。结果表明,建立的多重PCR检测方法分别对猪链球菌血清2型、7型和9型扩增出特异性片段,片段大小分别是360、600、800 bp。该方法特异性良好,与副猪嗜血杆菌(Haemophilus parasuis,Hps)、多杀性巴氏杆菌(Pasteurellamultocida,Pm)、波氏杆菌(Brodetella bronchiseptica,Bb)、沙门氏菌(Salmonella,Sal)、大肠杆菌(Escherichia coli,E.coli)以及猪链球菌其他29个血清型均无交叉反应。用建立的多重PCR方法对临床分离的341株猪链球菌菌株进行血清型分型鉴定,并与传统的血清凝集分型结果进行比较,二者符合率为97.36%。与传统的血清凝集方法相比,本研究建立的PCR方法具有快速、灵敏、特异等特点,可应用于实验室的快速诊断以及猪链球菌的流行病学调查。 相似文献
7.
宠物源大肠杆菌的血清型和毒力基因及耐药性调查 总被引：2，自引：0，他引：2
为研究宠物源致病性大肠杆菌(Escherichia coli)的血清型、毒力基因和耐药性之间的相关性,本研究由健康和患病犬、猫直肠拭子样品中分离177株E.coli,并采用玻片凝集法鉴定其血清型,结果显示分离株中定型菌株135株,分别属于20个不同的血清型,其中O1、O2和O8为主要的流行血清型。PCR方法检测11种毒力相关基因,并采用琼脂稀释法测定分离菌株对14种抗菌药的敏感性,结果表明3个血清型的菌株拥有相似的耐药表型,但毒力基因谱不同。62%的分离菌株携带fimH基因,并且毒力基因组合iroN+hlyF、iroN+fimH和traT+sitA比较流行。55%的O1血清型携带fimH基因,并且多数耐受四环素、多西环素、头孢噻吩和庆大霉素;20.8%的O2血清型菌株携带traT和sitA基因;50%的O8血清型携带traT和fimH基因。3个血清型分离菌株多数对安普霉素和阿米卡星敏感。 相似文献
8.
禽大肠杆菌病的病原为大肠埃希氏杆菌(Escherichiacoli),其血清型极多,其中菌体抗原(O)171种,荚膜抗原(K)103种,鞭毛抗原(H)56种,这些抗原可组合成大量抗原性不同的血清型。刘秀梵等〔1〕从我国18个省、市、自治区分离出440株禽源性大肠杆菌,经鉴定有60个血清型之多,而且不同地 相似文献
9.
10.
沙门氏菌是一种人畜共患病原体,迄今有2500多种血清型.它在宿主胞内和胞外环境中存活,其内毒素可引起有或无全身症状的肠道感染.尤其是一些无宿主特异性的沙门氏菌(如,肠炎沙门氏菌、鼠伤寒沙门氏菌)在公共卫生中的重要性日益凸现.沙门氏菌表面有鞭毛抗原H、菌体抗原O、荚膜抗原、菌毛抗原,再加上菌体本身对粘膜组织具 相似文献
11.
12.
13.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
16.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
18.
19.
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献