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种鸭化脓性关节炎病原分析 总被引：1，自引：0，他引：1
试验通过对湖北崇阳某种鸭场送检的患有关节炎的种鸭进行病理剖检,病变组织细菌分离,以及对分离菌的培养特性、生化特性和16 S rRNA核苷酸序列进行细菌鉴定,并对分离菌的致病性进行观察。结果显示:患病种鸭病理剖检眼观病变为化脓性关节炎,自病变关节分离并鉴定5种细菌,分别是化脓隐秘杆菌、葡萄球菌、大肠杆菌、乳房链球菌和粪肠球菌,其中除乳房链球菌外,其余4种细菌均可引起雏鸭关节炎。结果表明,该批种鸭由于化脓隐秘杆菌、葡萄球菌、大肠杆菌、粪肠球菌的混合感染而导致严重的化脓性关节炎。 相似文献
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以临床病死斑鸠为试验材料，采用病理学诊断方法：包括病理剖检、病理组织学观察等，以及病原学检测方法：包括细菌的分离培养、细菌16s RNA测序分析等，对该斑鸠病例进行检测。病理剖检结果显示：斑鸠肝脏肿大，质脆，轻度脂肪变性，表面有暗红的淤血区域以及稍有下陷的溃疡灶。病理组织学结果显示：肝索紊乱，叶间质增宽，并伴有嗜中性粒细胞浸润，可见多量肉芽肿结节。病原学检测结果显示：大肠杆菌和粪肠球菌混合感染。综合诊断该斑鸠病例为大肠杆菌粪肠球菌混合感染引起肉芽肿性肝炎和败血症致死。 相似文献
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1例雏鸭肝炎病毒和鸭圆环病毒混合感染的诊治 总被引：4，自引：0，他引：4
葫芦岛市某养鸭场樱桃谷肉鸭发生疑似雏鸭肝炎急性死亡病例。为了确诊病因,选取具有典型发病症状和病变的病鸭进行病原分离和鉴定,结果通过细菌的分离和攻毒试验排除了细菌感染为主要病因的可能;应用RT-PCR和PCR检测典型病变样品,结合扩增基因的测序,确诊发病鸭发生鸭肝炎病毒和鸭圆环病毒的混合感染。紧急应用鸭肝炎高免血清后发病鸭群死亡率得到有效控制,但存活鸭群中约有10%的雏鸭表现生长迟缓、消瘦,与鸭圆环病毒的感染症状相似。鸭圆环病毒是近年来新发现的一种病原,可导致感染鸭的免疫抑制,诱发混合感染,目前相关研究较少,值得关注。 相似文献
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熊源粪肠球菌小鼠感染模型的建立及其毒力基因的检测 总被引：1，自引：0，他引：1
建立了熊源粪肠球菌(Enterococcus faecalis)感染BALB/c小鼠模型,并对熊源粪肠球菌的毒力基因进行检测,同时观察死亡小鼠内脏器官的组织病理学变化。结果:熊源粪肠球菌可以引起小鼠内脏组织发生不同程度的炎性细胞浸润,其对小鼠的LD_(50)为2.04×10~7 cfu/只;通过PCR可检测出胶原蛋白黏附素(ace)、心内膜炎抗原(efaA)、EF3314和明胶酶E(gelE)等毒力基因。研究表明,本试验成功建立了粪肠球菌小鼠感染模型,为熊源粪肠球菌的发病机制研究奠定了基础。 相似文献
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致羔羊脑炎粪肠球菌分离鉴定及毒力因子基因的PCR检测 总被引：2，自引：2，他引：0
从新疆部分地区羊场近年来发生的以脑炎为主要临床特征的病死羔羊体内分离出2株细菌,经鉴定为粪肠球菌。经链球菌A-G乳胶分型诊断液检测后确定为D群链球菌。该菌对小鼠和羔羊有较强的致病性,人工感染可引起小鼠的死亡,感染羔羊能够复制出与自然感染相同的临床症状和病理变化,并从感染发病和死亡羊的脑、心血、肝脏中再分离到相同细菌,分离菌对万古霉素、氟苯尼考、头孢噻肟钠、诺氟沙星和恩诺沙星敏感,根据GenBank中粪肠球菌主要毒力因子的基因序列设计引物进行PCR检测,结果显示,1株细菌明胶酶（GelE）、聚合物质（Asa1）、心内膜炎抗原（efaA）、胶原蛋白黏附素（Ace）、新的表面蛋白1（EF3314）等毒力因子基因阳性;另1株细菌溶血素激活因子（CylA）、表面蛋白（esp）、聚合物质（Asa1）、心内膜炎抗原（efaA）、胶原蛋白黏附素（Ace）、2种新的表面蛋白1和2（EF0591和EF3314）等毒力因子基因为阳性。 相似文献
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从1羽发病雏鸡中分离到1株溶血性革兰氏阳性球菌。初步分离鉴定后经攻毒试验能够造成小白鼠和雏鸡死亡,具有致病性。药敏试验表明,该菌对多种药物均表现耐药性,仅对庆大霉素、青霉素、氧氟沙星敏感。通过BLAST软件分析该菌株的16S rRNA序列,确定该致病性细菌为粪肠球菌。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献