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101.
Twelve (54.5%) of 22 free-roaming dogs in Ishigaki Island had tick infestation identified as Rhipicephalus sanguineus. There were 121 ticks recovered and consisted of 28 females, 58 males, 22 nymphs and 3 larvae. Infection of dogs possibly with canine ehrlichial pathogens was examined by both indirect immunofluorescence assay and polymerase chain reaction (PCR). Two dogs of the 13 examined were sero-positive for the human granulocytic ehrlichia agent, and one of two dogs was PCR positive for Ehrlichia platys. This dog had platelet numbers slightly lower than normal value, however, no morulae were found within platelet on peripheral blood smear stained with Giemsa.  相似文献   
102.
OBJECTIVE: To evaluate trends in feedlot cattle mortality ratios over time, by primary body system affected, and by type of animal. DESIGN: Retrospective cohort study. ANIMALS: Approximately 21.8 million cattle entering 121 feedlots in the United States during 1994 through 1999. PROCEDURES: Yearly and monthly mortality ratios were calculated. Numbers of deaths were modeled by use of Poisson regression methods for repeated measures. Relative risks of death over time and by animal type were estimated. RESULTS: Averaged over time, the mortality ratio was 12.6 deaths/1,000 cattle entering the feedlots. The mortality ratio increased from 10.3 deaths/1,000 cattle in 1994 to 14.2 deaths/1,000 cattle in 1999, but this difference was not statistically significant (P = 0.09). Cattle entering the feedlots during 1999 had a significantly increased risk (relative risk, 1.46) of dying of respiratory tract disorders, compared with cattle that entered during 1994, and respiratory tract disorders accounted for 57.1% of all deaths. Dairy cattle had a significantly increased risk of death of any cause, compared with beef steers. Beef heifers had a significantly increased risk of dying of respiratory tract disorders, compared with beef steers. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that although overall yearly mortality ratio did not significantly increase during the study, the risk of death attributable to respiratory tract disorders was increased during most years, compared with risk of death during 1994. The increased rates of fatal respiratory tract disorders may also reflect increased rates of non-fatal respiratory tract disorders, which would be expected to have adverse production effects in surviving animals.  相似文献   
103.
The isolation of (+)-totarol as active compound against Mycobacterium tuberculosis is reported from Chamaecyparis nootkatensis outerbark.  相似文献   
104.
The purpose of this study was to determine the frequency of selected pathogens in the tissues of a group of feedlot cattle with chronic disease (most often respiratory disease and/or arthritis). Samples of lung and joint tissues from 49 feedlot animals that had failed to respond to antibiotic therapy were tested by immunohistochemical staining for the antigens of Mycoplasma bovis, Haemophilus somnus, Pasteurella (Mannheimia) hemolytica, and bovine viral diarrhea virus (BVDV). Mycoplasma bovis was demonstrated in over 80% of cases, including in 45% of joints and 71% of lungs tested. Mycoplasma bovis was the only bacterial pathogen identified in the joints. Haemophilus somnus and Pasteurella (Mannheimia) haemolytica were found in 14% and 23% of cases, respectively, and were confined to the lungs in all instances. Infection with BVDV was demonstrated in over 40% of cases. Mycoplasma bovis and BVDV were the most common pathogens persisting in the tissues of these animals that had failed to respond to antibiotic therapy.  相似文献   
105.
Although abalone fisheries in South Africa have existed since 1949, cultivation started by successful spawning of captured specimens in 1981 to produce spat and juveniles. Twelve abalone farms, with an estimated investment of US$12 million, have since been established on the coast of South Africa, with a projected production of 500–800 tons. While Haliotis midae could reach a maximum size of about 200 mm shell length at an age of over 30 years in the wild, farm production is concentrating on an average size of 100 mm after 5 years. Growth rates of 0.08–4.5% body weight day ?1 for abalone of 10–17 mm shell length have been found under stimulated farming conditions on formulated diets, while the corresponding feed conversion ratio (FCR) was 0.9–2.4. Growth rate, FCR and protein efficiency ratio decline at water temperatures above 20 °C. Anaesthetics, for example magnesium sulphate, are used to prevent injury during removal from tanks. Prostrate diatoms, such as Cocconeis sublittoralis (Hendey), Amphora proteoides (Hustedt) and Achnanthes brevipes (Agardh) are preferred after the larval stage (5–7 days, depending on temperature) are finished. South Africa abalone growers prefer formulated feeds in pellet form, which is convenience and cost favourable to farm operations and management. Although fish meal has been found to be the most suitable protein source for inclusion in formulated diets, plant proteins such as soy bean meal, cottonseed meal and sunflower meal presented good growth and apparent protein digestibility. The parasite sabellid polychaete, Terebrasabella heterouncinata, recently named by Australian and American researchers, is indigenous to a variety of South African intertidal molluscs and impedes growth by causing irritation beneath the mantle in abalone. Prevention to some degree is possible by high standards of hygiene and husbandry of abalone in the tanks, but new techniques to control large infestations now include ultrasound. In spite of a reputation for toughness, abalone meat frozen immediately after shucking is tender relative to red meat. The success of abalone farming in South Africa has been as a result of a high degree of cooperation between the private sector and government‐backed research institutions, and it is anticipated that this collaboration will continue.  相似文献   
106.
Seventy physeal fractures in horses were initially managed by euthanasia (18), stall confinement (25), application of a cast (7), or internal fixation (20). Of the 52 physeal fractures initially managed with stall confinement, a cast, or internal fixation, 23 (44%) healed and 13 (25%) of these horses became sound. The number of horses less than 4.5 months of age with pressure physeal fractures that became sound was significantly higher (p < 0.05) than the number of horses greater than 4.5 months of age. The number of horses with functional, pain-free limbs (sound horses) or functional limbs (lame horses) was not significantly different (p > 0.05) for Salter-Harris Type I, II, III, or IV pressure physeal fractures; however, critical examination for growth disturbances was not performed. The number of horses with pressure or traction physeal fractures of less than 5 days duration prior to presentation that healed or became sound was not significantly different (p > 0.05) when compared with those horses with fractures of greater than or equal to 5 days duration.  相似文献   
107.
Abstract. Anecdotal and circumstantial evidence have suggested that the Olsen test underestimates plant-available phosphorus (P) in basaltic soils in Northern Ireland. Therefore, the ability of this test to predict plant-available P in basaltic (and non-basaltic) soils was investigated by regressing Olsen-P data against herbage P indices calculated from plant tissue test data using the diagnosis and recommendation integrated system. The average Olsen-P concentration for a range of fields situated on basaltic soils was considerably lower than the average Olsen-P concentration for a range of fields situated on non-basaltic soils, and yet mean sward P status, as given by the herbage P indices, was similar for both groups of fields. Herbage P indices were also much better correlated with Olsen-P measurements in non-basaltic soils than in basaltic soils. Furthermore, at low Olsen-P values (≶9mgPL−1) some swards on basaltic soils were genuinely deficient in P, while others were sufficient or even in surplus for this nutrient. The results confirm that Olsen-P is inadequate as a predictor of plant-available P in basaltic soils. It is concluded that an alternative soil test is needed to provide a reliable assessment of plant-available P in basaltic soils, to prevent overuse of fertilizer and manure P and to minimize the amounts of P entering local watercourses.  相似文献   
108.
The effects of intramuscularly administered medetomidine and butorphanol (MB), and medetomidine, butorphanol, atropine (MBA) on glomerular filtration rate (GFR) were determined in six dogs as measured by 99m-Tc-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA) nuclear scintigraphy. Direct systolic, diastolic, and mean arterial blood pressures and heart rate were measured at regular time intervals before, during, and after GFR calculations. The mean GFR measurement following MB was significantly greater (4.44 ml/min/kg) than following MBA (3.82 ml/min/kg) or saline treatment (3.41 ml/min/kg). There was no significant difference between the mean GFR measurements following MBA injection and following saline injection. Diastolic and mean arterial pressures following MBA injection were significantly higher than the values recorded after either MB or saline alone. Heart rate following MB administration was significantly lower than that recorded for dogs receiving MBA or saline alone. The results of this study indicate that the administration of medetomidine in combination with butorphanol significantly increases total GFR in healthy dogs, while the administration of the combination of medetomidine, butorphanol, and atropine does not.  相似文献   
109.
应用多重PCR检测人工感染鸡呼吸道疾病的研究   总被引:3,自引:0,他引:3  
本文报道了IBV,NDV,ILTV,MG人工感染4周龄SPF鸡和非免疫鸡后,用多重PCR检测试验鸡的咽喉棉拭子和器官组织样品,并与传统的病原分离鉴定,血清学方法进行比较,多重PCR无论是对单一感染病原,还是对两种以上混合感染病原,其敏感性和检测速度都优于传统的鉴别诊断方法,具有较高的实用价值,可以直接应用于临床检测,服务于生产。  相似文献   
110.
Abstract – The reproductive biology of three species of rainbowfish (Melanotaeniidae) in northeastern Australian rainforest streams was investigated. Two species, Melanotaenia eachamensis and Cairnsichthys rhombosomoides are endemic to the area, whereas the third, M. splendida splendida, is more widespread. The species were all highly fecund, producing many hundreds of eggs between 1.10 and 1.24 mm in diameter. Melanotaenia eachamensis was the most fecund, produced the largest eggs of the three species, and consequently exhibited the greatest maternal investment (as measured by gonadosomatic index). The majority of reproductive effort occurred during the dry season, although reproductively active fish were present year-round for each of the species, but particularly so for M. s. splendida and C. rhombosomoides. No evidence for a role by temperature or photoperiod as environmental cues for reproduction was found, and it was suggested that gonad development was strongly tied to somatic growth. The concentration of reproduction to the dry season ensures that larvae are produced during a period of relatively stable and benign physical conditions. Comparison of temporal changes in gonadosomatic index values suggest that the spawning season of M. eachamensis , which occurs in high-elevation streams, is more restricted and commences about 1 month earlier than either other species. A similar phenology was observed for the M. s. splendida population found at high elevation and highlights the potential for spatial differences in stream productivity to influence life history. Note  相似文献   
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