Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean     

Linnajara DE VASCONCELOS MARTINS FERREIRA  Fernanda DE CARVALHO  J&#;lia FONSECA COLOMBO ANDRADE  Damiany P&#;DUA OLIVEIRA  Fl&#;vio Henrique VASCONCELOS DE MEDEIROS  Fatima Maria DE SOUZA MOREIRA 《土壤圈》2020,30(1):98-108

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.  相似文献   

Acute oral toxicity of zinc phosphide: an assessment for wild house mice (Mus musculus)     

Lyn A. HINDS  Steve HENRY  Nikki VAN DE WEYER  Freya ROBINSON  Wendy A. RUSCOE  Peter R. BROWN 《Integrative zoology》2023,18(1):63-75

Irregular plagues of house mice, Mus musculus, incur major economic impacts on agricultural production in Australia. The efficacy of zinc phosphide (ZnP), the only registered broadacre control agent for mice, is reported as increasingly variable. Have mice become less sensitive over time or are they taking a sub-lethal dose and developing aversion? In this laboratory study, the sensitivity of mice (wild caught; outbred laboratory strain) was assessed using oral gavage of a range of ZnP concentrations. The estimated LD₅₀ values (72–79 mg ZnP/kg body weight) were similar for each mouse group but are significantly higher than previously reported. The willingness of mice to consume ZnP-coated grains was determined. ZnP-coated grains (50 g ZnP/kg grain) presented in the absence of alternative food were consumed and 94% of wild mice died. Mice provided with alternative food and ZnP-coated wheat grains (either 25 or 50 g ZnP/kg grain) consumed toxic and non-toxic grains, and mortality was lower (33–55%). If a sublethal amount of ZnP-coated grain was consumed, aversion occurred, mostly when alternative food was present. The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed. Under laboratory conditions, ZnP-coated grains coated with a new higher dose (50 g ZnP/kg grain) were readily consumed. Consumption of toxic grain occurred when alternative food was available but was decreased. Our unambiguous findings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.  相似文献   

Effects of different five-day progesterone-based synchronization protocols on the estrous response and follicular/luteal dynamics in dairy cows     

Fernando LóPEZ-GATIUS  Irene LóPEZ-HELGUERA  Fabio DE RENSIS  Irina GARCIA-ISPIERTO 《The Journal of reproduction and development》2015,61(5):465-471

This study compared the responses shown by lactating dairy cows to four different P4-based protocols for AI at estrus. Cows with no estrous signs 96 h after progesterone intravaginal device (PRID) removal were subjected to fixed-time AI (FTAI), and their data were also included in the study. In Experiment I, follicular/luteal and endometrial dynamics were assessed every 12 h from the beginning of treatment until AI. The estrous response was examined in Experiment II, and fertility was assessed in both experiments. The protocols consisted of a PRID fitted for five days, along with the administration of different combinations of gonadotropin releasing hormone (GnRH), equine chorionic gonadotropin and a single or double dose (24 h apart) of prostaglandin F_2α. In Experiment I (40 cows), animals receiving GnRH at the start of treatment showed a significantly higher ovulation rate during the PRID insertion period while estrus was delayed. In Experiment II (351 cows), according to the odds ratios, cows showing luteal activity at the time of treatment were less likely to show estrus than cows with no signs of luteal activity. Treatment affected the estrous response and the interval from PRID removal to estrus but did not affect conception rates 28–34 days post AI. Primiparous cows displayed a better estrous response than multiparous cows. Our findings reveal acceptable results of 5-day P4-based protocols for AI at estrus in high-producing dairy cows. Time from treatment to estrus emerged as a good guide for FTAI after a 5-day P4-based synchronization protocol.  相似文献   

Quantification of flight times of aerial treatments targeting invasive species: the interplay of helicopter or drone with bait-delivery systems,flight speed and bait form     

Benjamin D. Hoffmann  Adam Tessmann  Greg Quinn  Faye Lawton 《Pest management science》2023,79(6):2050-2055

BACKGROUND

Aerial treatments for invasive species management are now common, but we are unaware of any work published in the scientific literature quantifying how the interplay of numerous factors affects flight time and therefore operational costs. Here, we analyse aerial treatment data collected from two ant-eradication programmes, quantifying how the relationships between flight time and area are influenced by numerous aircraft/delivery system/bait/flight speed combinations.

RESULTS

For bait dispersal by helicopters, and when swath widths are equivalent, side-mounted Isolair was significantly more efficient than the simultaneous use of two underslung buckets, and use of two buckets was slightly but not significantly more efficient than one bucket. In this scenario, delivery by Isolair was, on average, 39.8% and 31.5% more efficient than the use of one or two buckets, respectively. However, when the swath width used with Isolair was halved to 10 m and flight speed was increased slightly, flight time was significantly greater compared to the other configurations. For bait dispersed by drone, flights conducted using an upgraded Flight Management System (FMS) and greater flight speed but smaller swath width were significantly more efficient than flights using the older FMS and lower flight speed. Over 10 and 50 ha the helicopter was 2.87 and 4.82 times more time efficient than the drone.

CONCLUSION

We encourage practitioners to publish data from their aerial treatments, and to try new methods, to accelerate improvements in efficiency and reduce the costs of aerial treatments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Impact of the quality of organic amendments on size and composition of the weed seed bank     

B DE CAUWER  T D’HOSE  M COUGNON  B LEROY  R BULCKE  D REHEUL 《Weed Research》2011,51(3):250-260

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In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components     

Lien VANDE MAELE  Marc HEYNDRICKX  Dominiek MAES  Nele DE PAUW  Maxime MAHU  Marc VERLINDEN  Freddy HAESEBROUCK  An MARTEL  Frank PASMANS  Filip BOYEN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):325-328

The antibacterial potential of organic acids and essential oil components against Brachyspira hyodysenteriae, the causative pathogen of swine dysentery, was evaluated. Minimum inhibitory concentrations (MIC) of 15 compounds were determined at pH 7.2 and pH 6.0, using a broth microdilution assay. In addition, possible synergism was determined. MIC values for the three tested strains were similar. For organic acids, MIC values at pH 6.0 were lower than at pH 7.2. B. hyodysenteriae was most sensitive to cinnamaldehyde and lauric acid, with MIC values <1.5 mM. Most antibacterial effects of binary combinations were additive, however, for thymol and carvacrol, synergism could be observed. In vitro results demonstrate the antibacterial action of certain essential oil components and organic acids against B. hyodysenteriae.  相似文献   

Growth and metabolic responses of tambaqui (Colossoma macropomum) fed different levels of protein and lipid     

L.C. DE ALMEIDA  I.M. AVILEZ  C.A. HONORATO  T.S.F. HORI  G. MORAES 《Aquaculture Nutrition》2011,17(2):e253-e262

Growth and metabolism were investigated in juvenile tambaqui (Colossoma macropomum) fed with isoenergetic diets in four treatments with protein (P) lipid (L) in g kg^?1: P350L49; P301L81; P253L113 and P205L145, respectively. The experiments were carried out in the laboratory facilities. Fish growth, the intermediary metabolites (total sugar, lactate, pyruvate, ammonia, protein, fatty acids, triacylglycerols, amino acids and glycogen), and the enzyme activities of lactate dehydrogenase, malate dehydrogenase, glutamate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were assayed. Fish from treatments P350L49 and P301L81 depicted the best growth. Increase in glycolytic, glycogenolytic and lipolytic activities in liver was observed following dietary lipid increase. In fish fed with high lipid levels, the energetic demand was supplied by enhancement of muscular glycogenesis and lipolysis. The plasma metabolic profile reflected the blood function on metabolites delivery to tissues underlining biochemical adaptations because of changes in the feeding. In conclusion, the increase in dietary lipid plus protein decrease resulted in (i) reduction in fish growth, (ii) augment of liver glycolysis and glycogenolysis (iii) increase in muscular glycogenesis and (iv) predominance of lipolytic metabolism.  相似文献   

Effect of masking organoleptic properties of fat on diet self‐selection by the sparid Diplodus puntazzo     

P.F. ALMAIDA‐PAGÁN  M.D. HERNÁNDEZ  J.A. MADRID  J. DE COSTA  P. MENDIOLA 《Aquaculture Nutrition》2011,17(2):e48-e55

Fish are able to select a balanced diet according to their nutritional needs by choosing among incomplete feeds or even pure macronutrients. However, the relevance of both the organoleptic properties of diet and the postingestive signals that they produce remains unclear. Thus, sharpsnout seabream were allowed to select between diets containing different edible oils with their organoleptic properties masked by using gelatine capsules. Fish were fed capsules of two different colours so that they could associate the capsule colour with its corresponding postingestive effect. The longitudinal experiment included a first phase during which the fish were adapted to consuming the gelatine capsules. In a second phase, the fish were challenged with two different encapsulated diets: one comprising a complete diet containing fish oil and the other a fat‐free diet. Sharpsnout seabream showed a preference for the fish oil capsules (3.8 ± 1.1 g kg^?1 body weight (BW), 66.8% of total intake) over the fat‐free capsules, showing that they were able to associate the colour of the capsule with their nutritional content through postingestive signals. After that, the fish were challenged to select between the capsules containing the fish oil diet and capsules containing a vegetable oil (linseed or soybean), in which case they showed no preference between diets (2.4 ± 0.3: 2.1 ± 0.5 g kg^?1 BW of fish oil versus linseed oil capsules and 2.2 ± 0.2: 1.8 ± 0.6 g kg^?1 BW of fish oil versus soybean oil capsules), indicating that the fatty acid composition of the different oils was not sufficient to affect dietary selection through postingestive signals. So, in conclusion, when orosensorial information from food is absent, the fish are able to select between diets at a macronutrient level by using postingestive information. However, this information is not sufficient for distinguishing between diets that differ in the type of oil used.  相似文献   

Heat Transfer Performance of Ethanol Tree-Dimensional Inner Micro-Fin Heat Pipe     

DE Jun  XIN Ming dao 《保鲜与加工》2003,(4):115-117

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RELATION OF SEVERAL PEDOLOGICAL CHARACTERISTICS TO ENGINEERING QUALITIES OF SOIL   总被引：2，自引：0，他引：2  

D. DE LA  ROSA 《European Journal of Soil Science》1979,30(4):793-799

An attempt to predict engineering qualities which are needed in soil evaluation and to enhance nonagricultural use of soil survey data was made. Data from a variety of soils were subjected to simple correlation and multiple regression analyses to relate three pedologic characteristics: clay content, cation-exchange capacity (CEC), and organic matter content to two engineering qualitites: plasticity index, and optimum moisture. Simple correlation coefficients showed that clay content and CEC correlated significantly with the engineering determinations. The relationship between organic matter content and these engineering determinations was weaker. Multiple regression analyses showed that the three pedologic characteristics accounted for most of the variation in each of the engineering qualities, and that prediction was feasible.  相似文献