共查询到20条相似文献，搜索用时 62 毫秒
1.
2.
以高脚鸡、威宁鸡、乌蒙乌骨鸡(含白壳蛋鸡和绿壳蛋鸡)3个贵州地方鸡种为研究对象,构建品种DNA池,扩增NKX2-5基因第1外显子全部序列及第2内含子部分序列,采用直接测序法对3个品种(4个群体)的NKX2-5基因进行单核苷酸多态性检测,利用生物信息学软件预测不同多态性位点对NKX2-5基因mRNA二级结构、蛋白质二级结构的影响.结果表明,在3个品种(4个群体)中共检测到G108A、T288C、C400T、T420G和G429A 5个SNPs位点,其中,G108A位于非编码区;T288C、C400T位于第1外显子区;T420G和G429A位于内含子区.T288C和C400T均为错义突变,T288C突变导致丝氨酸(Ser)变为脯氨酸(Pro)、C400T突变导致丙氨酸(Ala)变为缬氨酸(Val),SNPs位点对于NKX2-5基因mRNA二级结构、蛋白质二级结构有一定影响. 相似文献
3.
利用限制性片段长度多态性聚合酶链反应技术对549只西农萨能和关中奶山羊群的AGPAT6基因遗传多态性与生长性状(体高、体长和胸围)的相关性进行研究.结果表明AGPAT6基因存在4个单核苷酸多态性:P1、P2、P4与P10位点分别位于5′UTR (g.152G＞C)、内含子2(g.8124G＞A)、外显子4(g.9263C＞G)和3′UTR(g.16436G＞A).经关联分析,P2、P4和P10三位点在西农萨能奶山羊和关中奶山羊品种中都属于中度多态,4个SNPs的不同基因型对两个奶山羊品种的生长性状影响均不显著(P＞0.05). 相似文献
4.
不同SC/NSC日粮对徐淮白山羊瘤胃发酵的影响 总被引：1，自引：0，他引：1
选取4只体况良好、体重(30±2.5)kg的徐淮白山羊,并安装永久性瘤胃瘘管,按4×4拉丁方设计,饲喂4种结构性碳水化合物(SC)与非结构性碳水化合物(NSC)比例不同的日粮(SC/NSC3.52、2.06、1.28、0.84),分别为A、B、C、D组,研究徐淮白山羊瘤胃发酵规律.结果表明D组瘤胃液pH值极显著低于其他3组(P＜0.01);D组瘤胃液氨氮浓度(NH3-N)极显著高于A、B组(P＜0.01);A组微生物蛋白(MCP)浓度极显著低于其他3组(P＜0.01);瘤胃液总挥发性脂肪酸(VFA)、乙酸及丙酸浓度,C组最高,但差异不显著(P＞0.05);D组丁酸浓度极显著高于A、B、C三组(P＜0.01);乙酸与丙酸之比差异不显著(P＞0.05).结果表明,SC/Nsc为1.28时徐淮白山羊瘤胃发酵最佳. 相似文献
5.
研究康肽(羊胎盘转移因子)对猫免疫功能的影响。将28只猫分为4组(即对照组,康肽低剂量组、中剂量组、高剂量组),灌胃不同剂量的康肽,于试验前、后对免疫球蛋白Ig G、Ig A、Ig M、补体C3、补体C4及WBC、LY、RBC进行测定比较,其中WBC、LY、RBC用自动血液细胞分析仪检测,Ig G、lg M、lg A和补体C3、补体C4分别用Ig G、lg M、lg A试剂盒和补体C3、补体C4试剂盒进行检测。结果表明,康肽能提高猫血清中免疫球蛋白(Ig G、Ig M、Ig A)、补体(C3、C4)和WBC、LY、RBC的含/数量。提示康肽具有正向调节作用,剂量越大,作用越好。 相似文献
6.
本试验选用A、B、C、D 4种商品化鸡传染性法氏囊病活疫苗免疫SPF鸡,进行攻毒试验以评估不同疫苗的免疫效果。10日龄SPF鸡分组后分别用A、B、C、D 4种商品化法氏囊病活疫苗按1羽份/只剂量接种免疫,21日龄时用1000 LD50剂量的法氏囊超强毒株(GD0104)进行攻毒,各组疫苗的保护率分别为100%、85%、100%、100%;35日龄时用1000 LD50剂量的法氏囊超强毒株(GD0104)进行攻毒,各组疫苗的保护率分别为100%、75%、95%、100%。免疫后抗体检测显示,抗体滴度水平D＞A＞C＞B,抗体转阳速度A＞D＞C＞B,免疫应激作用A、D＞B、C。综合考虑疫苗免疫攻毒后各项指标的变化,A、D疫苗免疫效果优于其他两种活疫苗,可以有效地为SPF鸡提供保护作用。 相似文献
7.
对流行于贵州省的牛膀胱肿瘤的调查表明:本病最显著的临床症状是长期间歇性血尿。在1570头屠宰牛中检出膀胱肿瘤257例(16.37%)。其中黄牛的检出率高达18.74%,水牛为0.57%。对328例牛膀胱肿瘤进行了病理形态学观察及组织学分类,并对其中3例作了电镜观察。各类型肿瘤出现的频率依次是:移行细胞癌126例,纤维瘤97例,血管瘤42例,平滑肌肉瘤42例,乳头状瘤30例,腺癌18例,横纹肌肉瘤16例,血管肉瘤12例,纤维肉瘤8例,鳞状细胞癌3例,粘液瘤2例,以及腺瘤、平滑肌瘤和未分化癌各1例。在64个病例中,每例同时存在上述的二或三种肿瘤成分。在28例中见肿瘤组织向淋巴结或/和其他器官转移。 相似文献
8.
《中国牛业科学》2017,(6)
[目的]为研究槟榔江水牛杂交后代的育肥效果。[方法]选择槟榔江水牛×摩拉、槟榔江水牛×尼里-拉菲杂交F1代水牛25头,按年龄和体重近似的原则分为4个育肥试验组,分别为公牛A组(5~6月龄,体重139.0±18.9kg)、公牛B组(8~9月龄,体重195.4±29.5kg)、公牛C组(8~9月龄,体重215.4±28.8kg),阉牛D组(5~6月龄,体重139.3±26.9kg),4个组粗饲料均为玉米青贮,精料添加量分别为A组(2kg/d)、B组(3kg/d)、C组(4kg/d),D组(3kg/d),在相同饲养管理条件下育肥100d,测定日粮采食量和增重性能。[结果]表明:(1)4组试验牛始重和末重差异显著(P0.05),年龄和体重相近的A与D组、B与C组间的始重和末重差异不显著(P0.05)。平均日增重分别为A组0.77±0.12kg/d、B组0.78±0.10kg/d、C组0.85±0.14kg/d、D组0.79±0.13kg/d,组间差异均不显著(P0.05)。(2)组间的粗料和精料采食量均无显著性差异(P0.05);精料重比差异显著,分别为A组2.64±0.45、B组3.92±0.51、C组4.85±0.95、D组3.91±0.79(P0.05)。(3)添加精料对槟榔江水牛杂交后代的增重效果好,4组牛均取得良好的经济效益,经济效益最高的是精料投入最少的A组(年龄最小)为1 260.63元,而精料投入高的C组效益较差为952.62元。 相似文献
9.
62只28日龄断奶布列塔尼亚兔用按窝配对法分为3组,各组性别比例与体重相近,C组为对照组,A组日粮中添加0.75%饲用复合酶制剂,B组日粮中添加0.1%饲用复合酶制剂,测定各组血清胰岛素(Ins)、胰高血糖素(GG)、甲状腺素(T3、T4)以及血糖和血尿素氮含量.A组、B组的T3含量均高于C组(P＞0.05);A组、B组的T4含量均显著低于C组(P＜0.05),两组胰岛素含量也比C组低(P＜0.05);A组、B组的GG含量与C组相比均有下降趋势(P＞0.05);A组、B组的血尿素氮含量分别比C组低3.3%和1.67%(P＞0.05);A组、B组的血糖含量与C组相比也有下降趋势(P＞0.05).提示饲用酶制剂对血清激素和部分生理指标有一定影响. 相似文献
10.
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
20.
Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献