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采用鸡传染性法氏囊病(IBD)病毒(IBDV-2512)研制了检测IBOV抗体的ELISA试剂盒。抗原最佳包被浓度为5.12μg/ml,被检血清最佳稀释度为1:200,酶标结合物最佳工作浓度1:1000.本试剂盒与美国KPIIBD-ELISA试剂盒比较,结果敏感性、特异性、重复性均达到美国同类产品水平,且易于操作.在4℃可稳定保存6个月、对北京地区4个非免疫鸡群的103只鸡测得其阳性率为54%,对45只SPF鸡的阳性率为0%。 相似文献
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鸡传染性喉气管炎王岗株病毒(ILTV)经SPF鸡肾细胞增殖,差速离心及蔗糖密度梯度离心纯化,电镜观察。以纯化的病毒4次免疫BALB/c鼠,按常规方法进行细胞融合,采用有限稀释法克隆,经ELISA筛选出3株(8E2、13G6、9C4)分泌抗ILTV特异McAb的杂交瘤细胞。特异性试验表明它们与鸡的传染性支气管炎、痘病毒、新城疫病毒、马立克氏病病毒、白血病病毒及鸡肾细胞均无交叉反应。在获得的三株单抗建立单抗夹心-ELISA法基础上,首次建立了快速检测喉气管炎感染的ELISA试剂盒,对ILTV纯病毒的最小检出量为31.0ng/ml病毒蛋白。以该试剂盒对黑龙江省哈尔滨地区、辽宁省沈阳、大连地区、山东省济南及上海等地6565份喉部株拭样品进行检测,共检出阳性1154头份。对部分ELISA阳性和阴性样品进行平行病毒分离,结果阳性符合率为100%(112/112),阴性符合率为95%(38/40)。研究表明,该试剂盒特异性强,重复性好。该方法的建立为鸡群疫病监测,免疫鸡群抗体水平检测提供了新的可靠方法。 相似文献
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彩鸡传染性法氏囊病(IBD)病毒(IBDV-2512)研制了检测IBDV抗体的ELISA试剂盒。抗原最佳包被浓度为5.12μg/ml,被检血清最佳稀释度为1:200,酶标结合物最佳工作浓度1:1000。本试剂盒与美国KPIIBD-ELISA试剂盒比较,结果敏感性、特异性、重复性均达到美国同类产品水平,且易于操作,在4℃可稳定保存6个月。对北京地区4个非免疫鸡群的103只鸡测得其阳性率为54%,对4 相似文献
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应用SephadcxG-200层析法纯化鸡减蛋综合症病毒,利用NC膜作为载体,成功建立了检测EDS-76血请抗体的斑点酶联免疫吸附试验(Dot-ELISA)。抗原包被浓度为2μg/ml,被检血清浓度为1:20,酶标兔抗鸡1gG浓度为1:200.底物溶液最适pH值为7.2。对A-F6个养禽场随机抽检血清160份,分别用Dot-ELISA、HI和AGP检测,Dot-ELISA检出阳性率为51.9%,HI检出阳性率为46.9%,AGP检出阳性率为31.9%。对140日龄鸡人工感染试验,测定抗体消长规律。本方法不需特殊仪器。适用于基层兽医部门和养鸡场对EDS-76的血清学诊断和流行病学调查。 相似文献
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采用四种方法:即①单克隆抗体检测抗原的酶联免疫吸附试验(Ag-ELISA)②检测抗体的酶联免疫吸附试验(Ab-EUSA)③表膜检查(BCE)④小白鼠接种(MI),检查了肯尼亚183头骆驼锥虫的循环抗原,BCE查出37头阳性20%),MI60头阳性(33%),Ag-ELISA63头阳性(34%),Ab-ELISA检出90头阳性(49%)。其中BCE未能检出的24头中,Ag-ELISA检出18头(75%)。所有阳性头数中,Ag-ELISA检出93%,Ab-ELISA95%。根据55头的结果,阳性与阴性之间Ag-ELISAOD值差异极显著(P<0.0001),Ab-ELISAOD值差异不显著。因此,用Ag-ELISA或结合BCE诊断伊氏锥虫感染比用AB-ELISA更理想。 相似文献
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用PPA—ELISA检测鸡痘病毒抗体的研究 总被引:7,自引:0,他引:7
建立了检测鸡痘病毒抗体的PPA-ELISA,用该法检测75份鸡血清样品,鸡痘病毒抗体检出率(21.33%)高于琼凝扩散试验(10.67%),经血清抗体吸收和重复性试验,证明PPA-ELISA法具有特异性强和重复性好特点,此外,对4种封闭液的封板结果表明,10%犊牛血清PBS/T效果最好,次之为0.25%白明胶PBS/T,0.5%牛血清白蛋白PBS/T,0.1%牛血清白蛋白PBS/T。 相似文献
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建立了检测鸡痘病毒抗体的PPA-ELISA。用该法检测75份鸡血清样品,鸡痘病毒抗体检出率(21.33%)高于琼脂扩散试验(10.67%)。经血清抗体吸收和重复性试验,证明PPA-ELISA法具有特异性强和重复性好特点。此外,对4种封闭液的封板结果表明:10%犊牛血清PBS/T效果最好,次之为0.25%白明胶PBS/T、0.5%牛血清白蛋白PBS/T、0.1%牛血清白蛋白PBS/T。 相似文献
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ELISA检测鸡传染性贫血病病毒抗体中的非特异性反应问题 总被引:2,自引:0,他引:2
为了监测CSIROSPF鸡群,经常用商业ELISA试剂盒检测鸡群的抗鸡传染性贫血病毒(CAV)抗体。近来发现个别鸡群的阳性率达18.2%,占总数的2.7%。但后来采血发现大部分可疑鸡都为阴性,可见前面的结果存在假阳性。 相似文献
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作者建立了两种抗原捕捉酶联免疫吸附试验方法(多抗和单抗AC-ELISA),用于滴定用不同宿主系统增殖的传染性氏囊毒病,这些宿主系统包括BGM-70传代细胞系。鸡胚成纤维细胞和鸡法氏囊,两种检测方法都有较高的特异性,但是多克隆AC-ELISA比单克AC-ELISA更加敏感(P〈0.05),结果还表明,滴定IBDV抗原的常规方法(细胞培养物和鸡胚)比多抗AC-ELISA更敏感。 相似文献
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分别用血清中和(SN)试验和单克隆抗体(TGEmAb和TGE/PRCVmAb)阻断酶联免疫吸附试验(B-ELISA)对81头美国进口猪血清作猪传染性胃肠炎(TGE)抗体检测。SN试验检出7份阳性血清,检出率为8.64%;B-ELISA试验检出7份PRCV抗体阳性血清,检出率为8.64%,无TGE阳性。SN试验检出7份TGE抗体阳性血清与B-ELISA试验检出的7份PRCV抗体阳性血清完全重合。结果证明,应用单克隆抗体进行的B-ELISA可鉴别诊断TGE与PRCV感染,优于SN试验 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献