共查询到20条相似文献，搜索用时 339 毫秒
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本试验采集蒙古马新鲜粪便样品,采用酚－氯仿抽提法和2种细菌基因组DNA提取试剂盒法进行样品中细菌基因组DNA的提取。通过DNA浓度和纯度的测定,并将其作为模板扩增细菌16S rDNA V3区特异性片段对提取效果进行比较,从而找出更适合蒙古马粪便中细菌基因组DNA的提取方法。结果显示,3种提取方法得到的基因组DNA均能用于PCR扩增的模板,其中B试剂盒法提取的DNA数量较多,且纯度高,更适合用于提取蒙古马肠道微生物细菌基因组DNA及后续的蒙古马肠道微生物多样性等分子生物学试验。 相似文献
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禽类血液中的蛋白质含量较高,用传统方法提取其血液中的基因组DNA比较繁琐、纯度不高,而且提取的产物中有大量蛋白质残留。本研究采用改良的CTAB法从白羽王鸽全血中提取全基因组DNA,并以传统的酚-氯仿抽提的方法和TAKARA公司基因组提取试剂盒法作对照。结果表明：qCTAB法可以得到大量的、较完整的基因组DNA,并且纯度达到2．00,浓度达到了144．67ng/μL,完全可以满足PCR扩增限制酶切等实验的要求。 相似文献
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【目的】研究从成熟石榴叶片中提取高质量基因组DNA的方法。【方法】根据成熟石榴叶片组织中富含多酚、多糖的特点,以峄城中国石榴种质资源圃20种石榴成熟叶片为材料,分别采用改良CTAB法(Ⅰ、Ⅱ、Ⅲ、Ⅳ)4种方法提取成熟石榴基因组DNA,通过琼脂糖凝胶、紫外分光光度计分析、ISSR扩增等方法检测所提取DNA的质量。【结果】改良的CTAB Ⅳ法能从各个石榴品种的成熟叶片中提取出基因组DNA,琼脂糖电泳胶孔干净,无拖带,且DNA的纯度和完整性都较好,OD260 nm/OD280 nm的比值均在1.8~2.0之间,无降解现象,其质量、产量都高于其他改良CTAB法。经ISSR- PCR扩增结果表明,此方法提取的基因组总DNA完全适于ISSR分析。其主要的步骤是：在石榴叶片的研磨中添加Vc、PVP等抗氧化物质,加入CTAB提取液后65 ℃水浴30 min后冷却至室温,离心条件为15℃,10 000 r/min,10 min,DNA粗提液用氯仿抽提3次,可获得高质量的DNA。【结论】改良的CTAB Ⅳ法能有效地从石榴成熟叶片中提取到高质量的基因组DNA。 相似文献
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《畜牧与兽医》2021,(6)
为建立一套从猪口腔中采集唾液并高效地从唾液中提取DNA的方法,选取约15日龄的仔猪和180日龄成年公猪各5头,分别用不同唾液采样工具来采集唾液样品并进行DNA提取,通过NanoDrop 2000和琼脂糖凝胶电泳检测DNA的浓度和纯度;同时以猪肌肉组织样品提取的DNA为参照,对比不同方法和不同日龄猪唾液提取的DNA质量差异,PCR扩增GAPDH基因以验证唾液DNA的可用性。结果显示:针对不同日龄的猪可以利用棉签和海绵采样工具进行唾液采集;建立的裂解法可以成功地从猪唾液中提取DNA,比人唾液DNA提取的碘化钾法质量更优;DNA纯度、浓度以及GAPDH基因扩增产物与肌肉组织提取的DNA效果相比趋近一致。提示:本文建立的猪唾液DNA的获取方法成功。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献