共查询到17条相似文献，搜索用时 156 毫秒
1.
2.
3.
4.
5.
胸膜肺炎放线杆菌溶血外毒素研究进展 总被引：1，自引：0，他引：1
猪传染性胸膜肺炎是由胸膜肺炎放线杆菌(APP)引起的一种呼吸道传染性疾病.根据荚膜多糖和脂多糖抗原表位不同,将胸膜肺炎放线杆菌分为15个血清型,不同血清型的菌株毒力大小不同,致病性也有强弱之别.研究发现与APP致病性有关的毒力因子包括荚膜多糖、脂多糖、外膜蛋白、转铁结合蛋白、溶血外毒素等,其中溶血外毒素是APP引起猪致病最主要的毒力因子,也是主要的保护性抗原.论文从猪传染性胸膜肺炎溶血外毒素的分子结构、致病机制、免疫原性几个方面对其进行综述,为获得以Apx为抗原制备减毒活疫苗提供参考资料. 相似文献
6.
7.
试验旨在考查布鲁氏菌分子标记、毒力基因缺失疫苗株YZ-2的生物学特性和免疫原性。对YZ-2菌落形态、生化特性、变异检查试验、毒力、遗传稳定性等生物学特性,以及免疫小鼠后体液免疫和细胞免疫水平进行试验,并与其亲本株S19进行比较。结果显示,YZ-2的形态及生化特性同亲本株S19相一致,但变异检查试验结果显示YZ-2由亲本株的光滑型变为粗糙型;毒力试验证实YZ-2的毒力与亲本株S19显著减弱,且YZ-2遗传稳定性良好。免疫原性试验证实,布鲁氏菌YZ-2在整体上具有与亲本株相似的免疫原性。结果表明,布鲁氏菌分子标记、毒力基因缺失疫苗株YZ-2的安全性较S19进一步提高,稳定性好,且具有与亲本株相近的免疫原性,有很好的应用前景。 相似文献
8.
无乳链球菌是常见的人兽共患病原菌,常导致新生儿肺炎、脑膜炎、败血症及奶牛乳腺炎。近年来,在水生动物体内也频繁分离到该菌,尤其是自2007年以来,该菌每年都在中国广东、海南、福建、广西等罗非鱼主要养殖区暴发流行,严重威胁着罗非鱼养殖业的健康发展。作者综述了当前国内外对鱼类无乳链球菌病的病原学、流行病学、症状、病理变化、毒力因子（荚膜多糖、CAMP因子、表面免疫原性蛋白、α蛋白、C5a肽酶）和防控等方面的研究进展,以期为中国鱼类无乳链球菌病的防控提供参考。 相似文献
9.
猪传染性胸膜肺炎(Porcine infectious pleuropneumonia)是由胸膜肺炎放线杆菌( Actinobacillus pleuropneumoniae, APP)引起的猪的呼吸道疾病.为了有效地控制本病,长期以来人们一直致力于研究高效、能提供强大保护力的疫苗,其中研究较多的是亚单位疫苗.人们发现把去除细胞的培养物上清液接种猪也可提供一定的保护力,于是对其中可能的毒力因子及具免疫原性的成分分别进行了分析.包括荚膜多糖、脂多糖内毒素、外膜蛋白、溶血素、转铁结合蛋白、蛋白酶以及所谓的渗透因子等. 相似文献
10.
为研究猪丹毒杆菌C43005株的菌种特性,制备了一批猪丹毒杆菌C43005株并对菌种的真空度、剩余水分、形态、培养特性、生化特性、血清学特性、毒力、免疫原性等参数进行检定,重点对该菌种的毒力进行重复试验并对影响毒力的因素进行分析。结果表明,菌种的真空度、剩余水分、形态、培养特性、生化特性、血清学特性、免疫原性均符合《中华人民共和国兽用生物制品规程》二〇〇〇版相关质量标准的规定,毒力试验结果明显受培养基质量和动物个体差异的影响。本研究可以为猪丹毒杆菌C43005株的制备和检定提供参考依据。 相似文献
11.
12.
为研制用于预防金黄色葡萄球菌性奶牛乳房炎的免疫疫苗,使用4株血清型为外膜多糖336型(336PS)、荚膜多糖5型(CP5)和荚膜多糖8型(CP8)强毒力金黄色葡萄球菌试制出金黄色葡萄球菌多价疫苗,并通过抗体水平监测、攻毒保护试验以及两个牧场的田间试验对免疫效果进行了评价。抗体水平监测显示,首免后30 d即可产生较高的抗体水平,二次免疫后有效抗体水平可持续到产后210 d;人工攻毒保护试验显示,疫苗对金黄色葡萄球菌引起的临床型乳房炎的保护率为89%;田间试验结果表明,该疫苗对奶牛临床型乳房炎的保护率在75.2%～83.3%之间,对隐性乳房炎的保护率在49.9%～52.2%之间,免疫组日产奶量较对照组平均提高1.26 kg以上。上述结果表明,所试制的疫苗可以显著降低泌乳牛金黄色葡萄球菌引起的临床型和隐性乳房炎发病率并提高产奶量。 相似文献
13.
Detection of capsular polysaccharide in milk of cows with natural intramammary infection caused by Staphylococcus aureus 总被引：1，自引：0，他引：1
Detection of capsular polysaccharide (CP) in milk of cows with natural intramammary infection caused by Staphylococcus aureus was attempted. Five quarters of 5 cows harboring S aureus strains that produce type-8 CP were selected. Using an ELISA with a monoclonal antibody, type-8 CP was not detected in extracts prepared from fresh milk collected aseptically. By contrast, CP was easily detectable after incubation of infected milk at 38 C for 20 hours. Quantitation of CP in extracts from incubated milk samples by use of ELISA indicated a great variation of CP expression by strains. Although an incubation step was necessary to detect CP, results of the study indicate that CP may be expressed in vivo during intramammary infection caused by S aureus. 相似文献
14.
新疆部分地区奶牛乳房炎金黄色葡萄球菌荚膜多糖分型的研究 总被引：1，自引：0，他引：1
为调查新疆奶牛乳房炎金黄色葡萄球菌主要流行血清型,本研究采用玻板凝集和双重PCR方法对不同地区奶牛乳房炎乳样中分离的117株金黄色葡萄球菌进行血清分型.结果表明荚膜多糖5型占10.26％(12/117),8型占13.68％(16/117),336型占64.96％(76/117),未分型菌株占11.11 ％(13/117).该研究为新疆奶牛乳房炎金黄色葡萄球菌疫苗菌株的筛选提供了依据. 相似文献
15.
为提取纯化屎肠球菌（E.faecium）荚膜多糖（CPS）并测定其多糖含量,试验采用高压法破碎菌体,加入核糖核酸酶（25 μg/mL）、脱氧核糖核酸酶（0.5 mg/mL）、溶菌酶（0.25 mg/mL）及蛋白酶K （1.25 mg/mL）等去除核酸和蛋白质,得到荚膜多糖粗提物,经过30 ku和100 ku超滤离心管超滤浓缩和DEAE Sepharose Fast Flow层析柱层析后,得到纯化的屎肠球菌荚膜多糖,再利用苯酚-硫酸法测定其含量。结果表明,成功从屎肠球菌中提取纯化出荚膜多糖,其含量为75.21%,初步确定了屎肠球菌荚膜多糖的提取纯化方法,为今后相关疫苗的研制奠定了基础。 相似文献
16.
In order to extract and purify the capsular polysaccharide of Enterococcus faecium and measure contents of polysaccharide, high pressure crushing methods and enzyme decomposition methods (RNase A (25 μg/mL), DNase (0.5 mg/mL), lysozyme (0.25 mg/mL) and protease K (1.25 mg/mL)) were used to remove the nucleic acids and proteins to obtain crude capsular polysaccharide, and centrifugal ultrafiltration with 30 and 100 ku ultrafiltration centrifuge tubes and DEAE Sepharose Fast Flow column chromatography were also adopted to obtain the purified capsular polysaccharides of Enterococcus faecium. Then the content of polysaccharide was determined by the phenol-sulfuric acid method. The results showed the capsular polysaccharide content was 75.21%. The results revealed that capsular polysaccharide ofEnterococcus faecium was extracted successfully. The method of extracting capsular polysaccharide in Enterococcus faecium was established initially. 相似文献
17.
Production and partial characterization of monoclonal antibodies to Pasteurella haemolytica A1 capsular polysaccharide and lipopolysaccharide 总被引：3，自引：0，他引：3
M V Penaredondo S K Maheswaran L O Whiteley T R Ames J R Leininger 《American journal of veterinary research》1988,49(11):1962-1968
Hybridoma-derived monoclonal antibodies (MAB) against the cell surface antigens of Pasteurella haemolytica serotype 1 were obtained by the fusion of murine myeloma cells (P3 X 63 - Ag 8.653) with splenocytes of BALB/c mice immunized with crude logarithmic growth-phase culture supernatant. Initial screening was performed, using an ELISA, with the same bacterial growth culture supernatant as coating antigens. Further selection was done, using a panel of purified antigens--either capsular polysaccharide or lipopolysaccharide--as the coating antigen in an ELISA, and then performing a leukotoxin-neutralization assay. Two MAB, designated IIB-6 and H-2, reacted specifically with the capsular polysaccharide and the other 3, designated IVG-3, IH-3, and IIC-2, reacted with the lipopolysaccharide. One MAB, designated IH-6, did not react with leukotoxin, capsular polysaccharide, or lipopolysaccharide. The MAB to the capsular polysaccharide (IIB-6 and H-2) were characterized further; both antibodies belonged to the IgM class and were agglutinating. In addition, they promoted neutrophil-mediated opsonophagocytosis and complement-mediated immune bacteriolysis of P haemolytica serotype 1. Results from 3 studies indicated that the MAB IIB-6 and H-2 were specific only to the capsular polysaccharide of serotype 1 of P haemolytica. The MAB to the lipopolysaccharide (IVG-3, IH-3, and IIC-2) were of the IgG1, IgG3, and IgM classes, respectively and were not characterized further. The availability of a MAB identifying a serotype-specific, surface-exposed determinant on the capsule of P haemolytica serotype 1 should facilitate and expand studies concerning the role of the capsular material and lipopolysaccharide in the pathogenicity of P haemolytica infection in cattle. 相似文献