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为探究重组枯草芽孢杆菌SE1制剂对肉鸡生长、免疫及抗氧化功能的影响,本研究将120羽1日龄肉鸡随机分为3组,每组4个重复,每个重复10羽:A组饲喂基础日粮,B组饲喂日粮中含枯草芽孢杆菌168制剂1.0×10~6 cfu/g,C组饲喂日粮中含重组枯草芽孢杆菌SE1制剂1.0×10~6 cfu/g。于饲喂实验28 d和42 d时分别测定肉鸡生长性能、免疫功能、肝脏和血清抗氧化能力。结果显示,重组芽孢杆菌SE1制剂与枯草芽孢杆菌168制剂对肉鸡生长、料重比、免疫器官指数没有影响(p0.05),但均可显著促进28 d时的外周血T淋巴细胞的增殖(p0.05),与A组相比,C组SE1制剂能够提高42 d时肉鸡肝脏过氧化氢酶和血清超氧化物歧化酶活力(p0.05),降低28 d时肉鸡肝脏和血清丙二醛含量(p0.01或p0.05);C组血清抗鸡白痢沙门氏菌OmpC IgG水平极显著高于A组和B组(p0.01),肠黏膜IgA水平也显著高于其它两组(p0.05)。结果表明重组枯草芽孢杆菌SE1制剂可提高肉鸡的免疫功能和抗氧化性能,诱导产生特异性抗鸡白痢沙门氏菌Omp C蛋白的血清IgG抗体和回肠粘膜IgA抗体。 相似文献
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为了有效防治鸡白痢沙门杆菌病,试验采用吉林市郊区数家鸡场分离、鉴定的致病性鸡白痢沙门杆菌,经固体增菌培养纯化后用甲醛灭活,制成油佐剂灭活抗原免疫健康蛋鸡;当抗体效价达到1∶320倍时,收集免疫鸡所产蛋分离卵黄,减压,低温干燥,制成卵黄抗体细粉,对试验雏鸡采用预防量饲喂后进行强毒菌攻毒试验、强毒菌攻毒后治疗量的饲喂试验及小区域雏鸡饲喂试验。结果表明:卵黄抗体粉预防添加量为3%时,攻毒保护率可达73.3%;治疗添加量为5%时,治愈率可达66.67%;卵黄抗体添加组较同期普通饲养组总发病率降低7%,抗生素用量降低11%,增重率提高6%。 相似文献
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《畜牧兽医学报》2016,(10)
为研究携带新城疫病毒(NDV)血凝素-神经氨酸酶(HN)基因疫苗的重组减毒鸡白痢沙门菌的口服免疫原性,将携带NDV HN基因的重组质粒pcDNA3-HN电转化减毒鸡白痢沙门菌ΔcrpC79-13,构建重组疫苗菌株ΔcrpC79-13(pcDNA3-HN)。将重组菌株ΔcrpC79-13(pcDNA3-HN)以1×10~9 CFU·只-1口服免疫7日龄雏鸡,同时设PBS、ΔcrpC79-13(pcDNA3)和NDVⅣ系疫苗免疫对照,于免疫后7、14、21、28、35d测定免疫雏鸡诱导的抗NDV的HI抗体、鸡白痢沙门菌IgG抗体和肠道黏膜IgA抗体动态水平,同时测定免疫雏鸡的外周血淋巴细胞增殖水平并进行攻毒试验。结果表明,成功获得携带NDV HN基因疫苗的重组菌株Δcrp C79-13(pcDNA3-HN);在免疫后14~35d,可诱导产生抗NDV的HI抗体,且在免疫后21d时达到最高值;可诱导产生抗鸡白痢沙门菌的IgG抗体,且在免疫后28d达到最高值;Δcrp(C79-13pcDNA3-HN)组肠道黏膜IgA抗体含量最高值略高于Δcrp C79-13(pcDNA3)组,但差异不显著(P0.05)。在免疫28d以后,Δcrp(C79-13pcDNA3-HN)组外周血淋巴细胞刺激指数极显著高于PBS对照组(P0.01),显著高于ΔcrpC79-13(pCDNA3)组(P0.05)。用103EID50 NDV F48E9株攻毒,保护率达67%(10/15),用108 CFU鸡白痢沙门菌C79-13攻毒,保护率为100%。本研究结果表明重组减毒鸡白痢沙门菌ΔcrpC79-13(pcDNA3-HN)具有良好的口服免疫原性。 相似文献
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《中国兽医学报》2020,(8)
NmpC是沙门菌编码的外膜蛋白,也是沙门菌诱导体液免疫的主要抗原。为研究沙门菌NmpC蛋白的免疫特性,本试验对沙门菌nmpC基因进行扩增,并将其克隆到pET-28a载体上,利用大肠杆菌表达系统表达重组蛋白rNmpC,进一步将rNmpC免疫小鼠,检测特异性抗体水平;用肠炎沙门菌C50336菌株感染rNmpC免疫小鼠评测其免疫保护效果。结果显示,成功表达和纯化出肠炎沙门菌NmpC重组蛋白,rNmpC大小为38 000;将rNmpC蛋白免疫小鼠,可以刺激小鼠产生高水平特异性抗体;细菌感染试验表明,rNmpC蛋白免疫小鼠可使其产生针对肠炎沙门菌感染的保护力,保护率为70%。本试验证实沙门菌NmpC蛋白的免疫潜力,为进一步研制沙门菌新型疫苗提供了参考。 相似文献
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枯草芽孢杆菌可以作为外源蛋白的表达系统,其作为重组蛋白的递呈载体具有很大优势,而纤维连接蛋白外域A(EDA)是一种能够提高目标蛋白抗原性的佐剂。为增强巨型艾美耳球虫的免疫相关蛋白1(IMP1)的抗原性,本研究从已构建的重组质粒中扩增EDA-EmIMP1和EmIMP1基因片段,并构建重组表达质粒pHT01-EDA-EmIMP1和pHT01-EmIMP1,将获得的重组质粒转化枯草芽孢杆菌感受态细胞,制备的重组枯草芽孢杆菌鉴定正确后增殖培养。将实验鸡分为5组,分别是:EDA组(免疫pHT01-EDA-EmIMP1重组枯草芽孢杆菌)、EmIMP1组(免疫pHT01-EmIMP1重组枯草芽孢杆菌)、枯草芽孢杆菌组、空白组(PBS)和攻虫对照组。每组鸡口服相应菌液(0.3mL1010cfu/mL菌液/只)进行免疫,每隔2周免疫1次,共免疫3次,每次免疫2周后采血。三免2周后攻虫。采用ELISA方法检测各组鸡血清中EmIMP1的抗体滴度以及细胞因子IL-10和IL-4的含量;并对攻虫后各组鸡的增重、肠道病变计分以及卵囊排出量进行计算。结果显示:制备的重组枯草芽孢杆菌可以表达目的蛋白,蛋白大小为70ku。ELISA检测结果显示,EDA组的抗体效价最高,约为1∶3200;三免EDA组的IL-10和IL-4的含量均显著高于其它组(p<0.05);EDA组的卵囊排出量显著低于其它组,而枯草芽孢杆菌组和EmIMP1组的卵囊排出量也均显著低于攻虫组(p<0.05);EDA组对肠道保护效果显著优于其它经过攻虫处理的组(p<0.05),而枯草芽孢杆菌组和EmIMP1组之间无显著差异;EDA组增重显著高于攻虫组和枯草芽孢杆菌组(p<0.05)。本研究证实枯草芽孢杆菌作为EmIMP1的抗原递呈载体可以有效提高免疫原性,其中EDA佐剂组的免疫效果最好。 相似文献
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鸡新城疫病毒HN基因亚单位疫苗诱导免疫保护的实验研究 总被引：3，自引：0，他引：3
实验分别将应用Bac to Bac系统表达的新城疫病毒四平株和长春株血凝素-神经氨酸酶基因（HN基因）蛋白的重组杆状病毒感染Sf-9昆虫细胞后28℃培养72h后，洗涤、收集昆虫细胞，离心浓缩，-20℃冻融3次，用HA-HI实验和HN单抗中和试验测定表达产物的活性，将表达的重组蛋白作为亚单位疫苗免疫鸡，用间接ELISA和HI试验测定鸡体内抗体效价，免疫后第15d用国家标准强毒NDVF48E8攻毒，统计免疫保护率，结果表明，表达的NDVHN重组蛋白能够诱导鸡体产生抗NDV特异性IgG抗体和HI抗体，实验Ⅰ组（四平株）雏鸡保护率为65%，实验Ⅱ组（长春株）雏鸡保护率为100%。 相似文献
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为评价大肠杆菌(E.coli)外膜蛋白酶T(OmpT)的免疫原性及对小鼠的免疫效率,本研究将原核重组表达纯化的OmpT蛋白(rOmpT)免疫小鼠,并以灭活的E.coli 308-2全菌疫苗株作为对照组,通过检测其抗体效价、体外调理吞噬作用和细胞因子表达水平以及攻毒试验进行保护效率评价。结果表明rOmpT免疫组在二免后14 d其抗体水平可以达到峰值(1∶64 000),并且其抗血清具有显著促进巨噬细胞对E.coli的吞噬作用;同时,重组蛋白与全菌免疫组均能够显著提高小鼠体内IL-4和IFN-γ的表达。而且重组蛋白免疫组对4个进化种系(Phylogenetic group)的E.coli攻毒的免疫保护率均为60%左右,比全菌免疫组高约10%。研究结果显示rOmpT比全菌灭活E.coli能够诱导小鼠产生更有效免疫保护作用,表明rOmpT可以作为E.coli亚单位疫苗候选蛋白。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献