共查询到20条相似文献，搜索用时 531 毫秒
1.
《中国兽医学报》2016,(4):665-668
免疫球蛋白λ多肽1(immunoglobulin lambda-like polypeptide 1,IGLL1)是一种具有编码蛋白质特性的基因,位于17号染色体上。通过本实验室前期的奶牛乳腺组织转录组测序研究表明,IGLL1基因可能对奶牛乳脂的合成代谢产生影响。本试验以中国荷斯坦奶牛乳腺组织为研究对象,选取3头高脂中国荷斯坦奶牛,3头低脂中国荷斯坦奶牛的乳腺组织提取RNA并反转录为c DNA,进行RT-PCR,并对数据进行统计分析,研究IGLL1基因在奶牛乳腺组织中的表达差异。结果显示,该基因在高脂奶牛及低脂奶牛的乳腺组织中均有表达,且高脂奶牛的乳腺组织中的表达量显著高于低脂奶牛的表达量(P=0.0370.05)。研究表明,IGLL1基因可能对中国荷斯坦奶牛的乳脂的合成或代谢产生影响。该研究为奶牛的优良品种选育提供了试验基础。 相似文献
2.
《中国兽医学报》2016,(6)
与奶牛乳脂相关的基因在奶牛乳腺组织中表达量的多少对奶牛的乳脂率起到关键的调控作用。C4BPA(complement component 4binding protein,alpha)基因是通过对高乳脂奶牛及低乳脂奶牛的乳腺组织进行转录组测序选择出的差异基因。为了研究C4BPA基因在奶牛乳脂合成代谢过程中的作用,本试验以中国荷斯坦奶牛为研究对象,利用乳成分分析仪检测15头中国荷斯坦奶牛的乳脂率,挑选出高乳脂奶牛与低乳脂奶牛各3头(P0.05),提取乳腺组织总RNA,反转录成cDNA,进行荧光定量PCR,检测该基因在奶牛乳腺组织中的表达量,并利用SPSS软件对不同奶牛乳腺组织中C4BPA基因的表达量进行差异分析。结果表明,C4BPA基因在不同奶牛乳腺组织中均有表达,且在高乳脂奶牛及低乳脂奶牛的乳腺组织中表达量差异显著(P0.05)。本试验为进一步对C4BPA基因生物学功能和作用机制的研究奠定基础。 相似文献
3.
试验旨在克隆中国荷斯坦奶牛CC趋化因子受体5(C-C chemokine receptor type 5,CCR5)基因,对其进行生物信息学分析,并探究CCR5基因在奶牛炎性和健康组织中的表达水平。采用PCR技术扩增并克隆荷斯坦奶牛CCR5基因CDS区全长序列,连接pMD18-T载体并转化大肠杆菌DH5α感受态细胞,通过蓝白斑方法筛选阳性克隆并测序,对序列进行相似性比对及系统进化树构建;应用多种在线生物信息学软件对其编码蛋白进行分析,并利用实时荧光定量PCR方法检测CCR5基因在健康和炎症奶牛乳腺组织中的表达情况。结果显示,中国荷斯坦奶牛CCR5基因CDS区全长1 059 bp,编码352个氨基酸。相似性和遗传进化分析结果显示,奶牛CCR5基因与绵羊的遗传距离最近,高达96.0%,与鸡遗传关系最远,为61.0%,且在不同物种之间CCR5基因高度保守。中国荷斯坦奶牛CCR5蛋白分子质量为40.235 ku,理论等电点(pI)为9.30,为疏水性蛋白但不是分泌蛋白,主要存在于细胞质内;在CCR5蛋白二级结构中α-螺旋和无规则卷曲分别占51.14%和32.95%,三级结构模型预测结果与二级结构一致。实时荧光定量PCR结果显示,CCR5基因在健康奶牛乳腺组织中的表达量极显著低于炎性奶牛乳腺组织(P<0.01),提示其可能参与奶牛乳腺炎的发生过程。本试验结果为进一步研究奶牛CCR5蛋白的功能提供了理论依据,对探究奶牛CCR5基因在奶牛乳腺炎中的调控功能等具有重要意义。 相似文献
4.
5.
试验旨在快速有效地检测泌乳奶牛的二酰甘油转酰基酶1（diacylgycerol acyltransferase 1,DGAT1）乳脂量性状的优势等位基因K232A单核苷酸多态性,确定DGAT1基因型进而确定泌乳性状,为中国荷斯坦奶牛分子标记辅助选择提供技术支持。选取6头泌乳初期（3头为高乳产量牛,3头为低乳产量牛）中国北方荷斯坦奶牛为研究对象,提取乳腺组织基因组DNA,分别设计1对外引物和1对内引物,建立一种四引物ARMS-PCR体系快速检测奶牛乳腺组织DGAT1基因单核苷酸多态性。结果发现,外引物扩增片段长度为512 bp,为PCR反应的阳性对照,基因型为232K扩增片段长度为369 bp,基因型为232A扩增片段长度为181 bp。PCR结果显示,6头牛的乳腺组织样本均由外部引物扩增出长度为512 bp的片段,泌乳期高乳产量奶牛和泌乳期低乳产量奶牛乳腺组织的特异性扩增片段长度均为181 bp。表明本研究选取的6头奶牛样本DGAT1基因K232A多态性均为232A型。提示该PCR鉴定方法能够快速有效地鉴定奶牛DGAT1基因型,可用于中国荷斯坦奶牛分子标记辅助选择。 相似文献
6.
【目的】 探究CC趋化因子受体7(CC chemokine receptor 7,CCR7)基因编码蛋白的生物学特性及其mRNA在健康和炎性奶牛乳腺组织中的表达量。【方法】 使用PCR扩增并克隆中国荷斯坦奶牛CCR7基因CDS区,测序后进行相似性比对及系统进化树构建;使用多种生物信息学软件分析CCR7蛋白的组成、理化性质及结构等;使用实时荧光定量PCR技术检测CCR7基因在健康和炎性奶牛乳腺组织中的表达差异。【结果】 中国荷斯坦奶牛CCR7基因CDS区全长1 325 bp,与绵羊的相似性较高(95.2%),亲缘关系较近;与鸡的相似性较低(56.4%),亲缘关系较远。CCR7基因编码379个氨基酸,其中亮氨酸含量最多,缬氨酸含量次之,组氨酸和色氨酸含量最低;CCR7蛋白分子质量为14.56 ku,理论等电点为8.89,平均亲水系数为0.640,是一种跨膜疏水性蛋白。CCR7蛋白信号肽切割位点可能存在于第24和25位氨基酸之间。CCR7蛋白二级结构中α-螺旋占比最大,高达51.72%,三级结构主要为α-螺旋和无规则卷曲。CCR7基因在炎性奶牛乳腺组织中的表达量极显著高于健康乳腺组织(P<0.01),其可能在奶牛乳腺炎的免疫调控中有着重要的作用。【结论】 试验成功克隆出牛CCR7基因CDS区序列,并进行了相应的生物信息学分析及组织定量表达,为进一步研究CCR7基因的具体功能提供材料,对于探究CCR7基因在奶牛乳腺炎中的调控功能具有重要的意义。 相似文献
7.
本试验通过对10头荷斯坦奶牛在受孕前后的整合素αvβ3表达变化规律与奶牛早期妊娠关系的研究,筛选早期妊娠检测标志性因子,从而提高奶牛的繁殖效率。试验表明,选取的10头荷斯坦奶牛中有7头受孕3头未受孕,检测发现,10、13、16、19d,αv基因和β3基因转录及整合素αvβ3表达水平在受孕奶牛血液中的表达量均高于未孕奶牛,特别是输精后第16d受孕奶牛的表达量显著高于未孕奶牛。因此,αvβ3检测可将早孕检测的时间窗口提前到人工输精后第16d。 相似文献
8.
9.
本研究旨在探究荷斯坦奶牛乳腺干细胞的多向分化潜能及催乳素对其增殖活性的影响。采用尼氏染色法对乳腺干细胞向神经细胞分化的情况进行鉴定,利用RT-PCR技术对诱导后的乳腺干细胞进行基因水平检测;MTT法检测催乳素对乳腺干细胞增殖活性的影响。结果表明:荷斯坦奶牛乳腺干细胞经成神经诱导后,细胞中有类似神经元的细胞出现,并且细胞周围出现许多微管样的结构,经尼氏染色呈阳性。诱导后神经细胞标记基因NSE和β-Tubulin Ⅲ呈阳性表达。催乳素刺激可促进乳腺干细胞的增殖,低浓度时乳腺干细胞的活力较好,且100ng·mL~(-1)为增殖最适浓度。 相似文献
10.
目的:研究荷斯坦奶牛乳腺发育中乳糖的变化规律.方法:高效液相色谱法测定荷斯坦奶牛乳腺发育中乳糖的含量.结果:围产期,乳腺中才出现可检测到的乳糖;泌乳期乳糖含量增高,泌乳140d达到峰值;退化期乳腺乳糖含量迅速降低,退化30d含量很少.结论:不同生理时期,荷斯坦奶牛乳腺中的乳糖含量不同. 相似文献
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
20.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献