共查询到20条相似文献，搜索用时 62 毫秒
1.
2008年7月末,我国青岛即墨地区患病的虎皮鹦鹉幼雏出现体重下降、脱羽和羽毛变形萎缩、鸟喙变形及胸腺结构变异等症状,怀疑为鹦鹉喙羽病(PBFD)。利用PCR对濒死鹦鹉进行鹦鹉喙羽病病毒(PBFDV)的检测,并对扩增的C1基因进行了测序与分析。结果表明,有两只检测为PBFDV阳性,B last分析发现,QD-CN08株与GenBank中已发表的PBFDV分离株C1基因同源性为82%~93%,进化树分析表明与日本的毒株有比较近的亲缘关系。结合临床症状、流行病学特征和实验室诊断,确诊为鹦鹉喙羽病,此病为中国大陆首次报道。 相似文献
2.
2018年山东省某养殖场发生大量幼龄鹦鹉死亡事件,疑似为病毒感染。为探寻病因,开展了该场及省内另外两个鹦鹉养殖场的流行病学调查。利用PCR技术对临床样品中提取的DNA或RNA进行检测,结果发现新城疫与禽流感病毒均呈阴性,而禽多瘤病毒(APV1)与鹦鹉喙羽病病毒(PBFDV)呈现为强阳性,由此推断此3个鹦鹉养殖场存在APV1和PBFDV感染,平均病毒检出率分别为67.9%与71.4%,共感染检出率率为58.0%。对阳性样品进化全基因分析发现:区域内的PBFDV流行毒株同源性高,与该地区早期报道的毒株亲缘关系较为接近;APV1的VP1基因同源性较高,与欧洲分离毒株亲缘关系较为接近,说明我国流行的APV1或来源于进口鹦鹉。本研究警示,需要加强鹦鹉疾病防控,严格鹦鹉进出口检疫,并制定和健全标准的鹦鹉病检疫程序。 相似文献
3.
鹦鹉喙羽症病毒(PBFDV)是圆环病毒科圆环病毒属的成员,可引起多个品种鹦鹉的急性死亡或者羽毛脱落和喙变形。病毒基因组中的ORF1和ORF2分别编码病毒复制相关蛋白(Rep protein)和衣壳蛋白(Cap protein)。Cap蛋白是圆环病毒的主要结构蛋白,也是其主要的免疫保护性蛋白。本试验应用PCR技术选择性扩增PBFDV Cap蛋白抗原表位集中区域的基因片段,并将其克隆入原核表达载体pGEX-6p-1中,诱导表达截短的Cap蛋白。SDS-PAGE及质谱鉴定结果显示,融合表达的CapT蛋白约45 kDa,切胶纯化后的蛋白浓度约1. 6 mg/mL。该Cap蛋白的成功获得为PBFDV治疗用阳性血清制备及亚单位疫苗的开发奠定了必要的物质基础。 相似文献
4.
为评价PCR结合斑点杂交技术在鸡REV检测中的应用价值,采用PCR法制备禽网状内皮细胞增生症病毒特异性地高辛标记DNA探针,同时用PCR技术、斑点杂交方法和PCR产物斑点杂交方法检测了不同地区的病、死鸡的组织样品REV的感染情况。结果表明,PCR产物斑点杂交法的检出率(45.16%,14/31)高于组织DNA直接斑点杂交法(32.26%,10/31),显著高于单纯PCR扩增法(0%,0/31)。PCR结合斑点杂交检测技术能快速、敏感、准确,充分避免PCR中的假阴性和假阳性现象,值得推广应用。 相似文献
5.
为了解2017—2018年鹦鹉喙羽病在福建省某些地区鹦鹉中的流行情况,收集了福建省部分地区298份鹦鹉粪便样品,采用PCR方法进行粪便样品检测,对其中鹦鹉喙羽病病毒检测呈阳性的5个地区的样品进行衣壳蛋白(Cap)基因测序后比较其同源性,绘制系统进化树,分析氨基酸序列,并通过生物信息学及序列分析软件预测Cap蛋白二级结构及B细胞抗原表位。结果显示:鹦鹉喙羽病病毒的平均阳性率为41.28%,福州市某动物救助站和福州动物园的阳性率较高,分别为65.17%和64.29%,其次为三明动物园、福州市花鸟市场和福州市某鹦鹉繁殖基地,南平动物园的阳性率最低;所测5个毒株Cap基因与GenBank中新西兰株(AY518913)亲缘关系较近;Cap蛋白有丰富的二级结构和多处抗原指数较高的区域,具有潜在的B细胞抗原表位,位于5~27、110~127和141~153位氨基酸残基或其附近。研究结果对防控鹦鹉喙羽病,保障鹦鹉健康养殖有重要参考意义。 相似文献
6.
7.
根据GenBank上发表的curli菌毛csgC基因序列设计了1对特异性引物.从患乳腺炎的奶牛乳汁中分离出致病性大肠杆菌,经生物学鉴定后,提取全基因组DNA为模板,PCR扩增出csgC基因,连入pMD18-T克隆载体,测序,结果表明,扩增片段含有333个核苷酸,编码111个氨基酸的成熟蛋白,与已报道的大肠杆菌W3110的全基因组DNA中的csgC基因序列最相近,氨基酸序列同源性为99.7%.该基因与原核表达载体pET32a+相连,转入BL21(DE3)感受态细胞.挑选阳性菌落经PCR鉴定和酶切鉴定后表明成功构建原核表达载体pET32a+/csgC. 相似文献
8.
9.
用地高辛标记的核酸探针检测猪伪狂犬病毒野毒感染的研究 总被引：2，自引：0，他引：2
利用PCR技术从带有伪狂犬病毒（PRV）gE基因的重组质粒pMD18-T-gE中扩增回收约304bp大小的片段,并制备出地高辛标记的gE基因核酸探针。特异性检测结果表明,该探针能与重组质粒DNA发生特异性杂交,而与对照的PRVBartha-k61株疫苗毒DNA、猪细小病毒（PPV）DNA、猪圆环病毒（PCV）DNA、猪繁殖与呼吸综合征病毒（PRRsV）cDNA、猪瘟病毒（CSFV）cDNA的杂交反应均为阴性;敏感性检测结果表明,该探针对PRV野毒的最低检出量为4pg。应用该探针对11份繁殖障碍病料进行了杂交检测,共检出4份阳性病料,该结果与PCR检测结果一致,表明该核酸探针可用于猪伪狂犬病野毒感染的临床诊断。 相似文献
10.
《动物医学进展》2020,(5)
建立一种鹦鹉幼稚病(BFD)PCR诊断方法,为临床快速确诊鹦鹉幼稚病提供技术手段。参考GenBank中公开的BFDV基因组序列,设计了2对用于阳性质粒构建引物和1对检测引物,采用套式PCR从临床疑似病料中扩增BFDV VP1基因,构建VP1基因阳性质粒,以阳性质粒为模板,优化检测引物扩增体系与条件,进行灵敏度试验、特异性检测和临床样品检测,挑选3份不同地区阳性样品测序验证。结果显示,成功构建了BFDV VP1基因阳性质粒pGEM-T-VP1,建立的PCR检测方法检测下限为105.8copies/μL。用该方法检测时仅能以pGEM-T-VP1为模板扩增到目的条带,检测5种常见禽病毒均为阴性。应用建立的方法对75份疑似病料进行检测,结果19份为阳性,阳性率为25.3%。测定了3株不同地区BFDV流行毒株VP1基因片段序列,比对分析发现核苷酸序列同源性为99.4%～100%,在基因进化树上这3株病毒处在一个独立的分支上,显示出一定的地域特征。成功建立了一种灵敏度高、特异性好的BFD PCR诊断方法,应用于临床诊断显示出良好效果。 相似文献
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
20.
Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献