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为了通过比较培养筛选出能改进和提高山羊卵母细胞体外成熟效率的培养体系 ,培养的山羊卵母细胞以 TCM-199为基础培养液 ,添加 :(1) 10 %血清 (胎牛血清 (FBS)或发情山羊血清 (EGS) ) 2 0 m g/ L促黄体素 (L H) 10 mg/ L促卵泡素 (FSH) 1m g/ L 雌二醇 (E2 ) ;(2 ) 10 % EGS 促性腺素 (L H∶ FSH=5 mg/ L∶ 0 .5 m g/ L 或 2 0 mg/ L∶ 10mg/ L )或者 0 .0 75 IU / m L人绝经期促性腺素 (HMG) 1mg/ L estradiol 17β;(3) 10 % EGS 0 .0 75 mg/ L HMG 10～ 2 0 μg/ L EGF。此外 ,以 M199 10 % EGS 0 .0 75 mg/ L HMG 10～ 2 0 μg/ L EGF为培养基 ,溶解于自制超纯水或储存的商品化超纯水来培养卵母细胞。培养条件为 38℃ ,5 % CO2 。培养 2 4 h后 ,在体式显微镜下统计处于 M 期的卵母细胞比例。结果显示 :在卵母细胞成熟液中添加 10 % EGS比 10 % FBS的成熟培养效果好 ;添加 HMG能够促进卵母细胞的成熟 ,其效果比添加不同比例的 L H/ FSH好 ;成熟液中添加 10～ 2 0μg/ L EGF能促进卵母细胞的成熟 ,但成熟率没有明显提高 ;新鲜的超纯水对于卵母细胞的培养是必要的。结论 :新鲜超纯水配制的 M199 10 % EGS 0 .0 75 IU / m L HMG 10～ 2 0μg/ L EGF培养液可以获得最佳卵母细胞培养效果 相似文献
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研究旨在通过添加新型生长刺激剂促进酵母生长，提升酵母的生长效率。通过向酵母培养基中添加发酵桔皮，探究发酵桔皮对酵母生长的影响，利用单因素试验、正交试验、响应面分析等方法对添加发酵桔皮的酵母培养基进行优化。结果表明：添加发酵桔皮后的优化培养基为：葡萄糖100.756 g/L、蛋白胨8 g/L、酵母浸膏11.906 g/L、发酵桔皮（干物质含量）1.906 g/L、无水硫酸镁2 g/L、尿素2 g/L、磷酸二氢钾5 g/L。培养基优化后酵母生物量达到最大，比未添加发酵桔皮的培养基提高了1.3倍。发酵桔皮可以作为酵母生长刺激剂提高酵母活菌浓度，缩短酵母进入对数生长期的时间，从而促进酵母饲料工业降本增效。 相似文献
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为了研究Ghrelin是否参与影响体外受精(IVF)过程,试验运用体外成熟及体外受精技术,向体外受精培养体系中外源性地添加Ghrelin(0μg/L、300μg/L、600μg/L、900μg/L、1 200μg/L)及已被多项研究作为GHS-R的颉颃剂D-Lys3-GHRP-6(0μg/L、10μg/L、100μg/L、1 000μg/L)的方法,对Ghrelin及其受体颉颃剂D-Lys3-GHRP-6对绵羊卵母细胞体外受精情况进行检测,初步确定Ghrelin在绵羊体外受精过程中可能扮演的角色。结果表明:外源性添加的Ghrelin在绵羊卵母细胞体外受精过程中并没有发挥明显的生理作用,说明Ghrelin能促进卵母细胞的体外成熟而不能促进体外受精。 相似文献
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采用响应面法对乳双歧杆菌Z-1冷冻干燥保护剂配方进行优化。通过Plackett-Burman试验与最陡爬坡试验确定影响乳双歧杆菌Z-1冷冻干燥后活菌数的显著因素及最优响应值区间，再通过Box-Benhnken试验优化得到乳双歧杆菌Z-1冷冻干燥保护剂最佳配方。结果表明：海藻糖、蔗糖、L-半胱氨酸盐酸盐添加量是影响乳双歧杆菌Z-1冷冻干燥后活菌数的显著因素；经响应面试验设计建立活菌数和海藻糖、蔗糖、L-半胱氨酸盐酸盐添加量的回归模型，得到乳双歧杆菌Z-1冷冻干燥保护剂最佳配方为海藻糖添加量200.51 g/L、蔗糖添加量46.16 g/L、L-半胱氨酸盐酸盐添加量2.31 g/L，其他成分不变（脱脂乳粉添加量100 g/L、乳清蛋白粉20 g/L、甘油4 g/L、异抗坏血酸钠10 g/L、谷氨酸钠8 g/L）。 相似文献
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金丝桃素对小鼠生长性能脏器指数及盲肠微生物多样性的影响 《畜牧与饲料科学》2022,43(5):7-12
[目的] 探讨金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响。[方法] 将100只4周龄雌性昆明小鼠随机分为5组,每组20只;金丝桃素通过饮水给予小鼠,添加量分别为0、1、2、4、8 g/L;正饲期28 d,记录小鼠体重和采食情况,计算平均日增重、平均日采食量和料重比;记录死亡小鼠数量,计算死亡率;试验结束时用颈椎脱臼法处死小鼠,采集并称量心脏、肝脏、肾脏和脾脏,称重并计算脏器指数;采集盲肠内容物,利用16S rDNA高通量测序法分析盲肠微生物多样性。[结果] 饮水中添加1、2、4 g/L的金丝桃素可提高平均日增重（P>0.05）,4 g/L组平均日增重最高,比0 g/L组增加21.21%;饮水中添加金丝桃素可提高平均日采食量（P>0.05）,2 g/L组平均日采食量最高,比0 g/L组增加18.30%;4 g/L组的料重比最低（P>0.05）,比0 g/L组降低9.24%。1 g/L组、2 g/L组、4 g/L组、8 g/L组的心脏指数显著（P<0.05）高于0 g/L组,4 g/L组的肾脏指数显著（P<0.05）低于1 g/L组。盲肠菌群在门（Phylum）水平上,以厚壁菌门（Firmicutes）相对丰度最高,其次为拟杆菌门（Bacteroidetes）、变形菌门（Proteobacteria）,3个菌门占细菌总数的97.58%。在属（Genus）水平上, 普雷沃菌属（Prevotella）、拟杆菌属（Bacteroides）、考拉杆菌属（Phascolarctobacterium）相对丰度较高;1 g/L组、2 g/L组、4 g/L组的普雷沃菌属（Prevotella）和巨单胞菌属（Megamonas）的相对丰度显著（P<0.05）低于0 g/L组,副瘤胃球菌属（[Ruminococcus]）显著（P<0.05)高于0 g/L组;4 g/L组盲肠副拟杆菌属（Parabacteroides）、瘤胃球菌属（Ruminococcus）、乳杆菌属（Lactobacillus）显著（P<0.05)高于0 g/L组。[结论] 在饮水中添加金丝桃素在一定程度上有助于提高小鼠采食量和增重,显著提高心脏指数,增加盲肠内有益菌的相对丰度,对小鼠健康表现出有益作用。 相似文献
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《中国兽医杂志》2019,(9)
为建立止痢型中兽药液体制剂中非法添加黏菌素的高效液相-二极管阵列色谱法,采用十八烷基键合硅胶为填充剂,流动相为乙腈∶硫酸钠磷酸水溶液(20∶80),硫酸钠磷酸水溶液∶硫酸钠水溶液(7 g/L)∶磷酸水溶液(6.8%)(50.5∶22.75)。检测波长为215 nm。结果显示,在该色谱条件下,3种止痢型中兽药液体制剂本底色谱峰对黏菌素E_1的测定无干扰;黏菌素E_1在0.04～2 mg/mL浓度范围内线性关系良好;杨树花口服液的检出限为10 mg/mL,板蓝根注射液及小檗碱注射液的检出限均为4 mg/mL;上述3种制剂的添加回收率在95.9%～97.9%之间,表明该方法回收率较好,准确度较高,可用于止痢型中兽药液体制剂中非法添加黏菌素的定性定量检测。 相似文献
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《黑龙江畜牧兽医》2020,(17)
为了研究茶多酚和花青素对湖羊精液常温保存效果的影响,试验通过假阴道法采集健康湖羊精液,在基础稀释液中单独添加不同质量浓度的茶多酚(0,0.02,0.04,0.08,0.16 g/L)和花青素(0,0.01,0.03,0.05,0.07 g/L)以及0.08 g/L茶多酚+0.01 g/L花青素的联合添加进行常温(17℃)保存,每隔8 h检查1次精子活力、存活率、质膜完整率、畸形率。结果表明:添加茶多酚和花青素均能提高精液品质,而且单独添加0.08 g/L的茶多酚和0.01 g/L的花青素效果最佳。精液保存16 h后,0.08 g/L的茶多酚组精子活力、存活率、畸形率、质膜完整率分别为0.644 5,71.94%,2.08%,70.27%;0.01 g/L的花青素组精子活力、存活率、畸形率、质膜完整率分别为0.691 3,81.50%,18.33%,80.41%;0.08 g/L茶多酚+0.01 g/L花青素的联合添加组精子活力、存活率、畸形率、质膜完整率分别为0.588 9,90.98%,3.95%,66.94%。说明茶多酚和花青素能有效提高湖羊精液常温保存效果,且最适添加量分别为0.08 g/L和0.01 g/L,单独添加0.08 g/L茶多酚或者0.01 g/L花青素对精液的保存效果要优于0.08 g/L茶多酚+0.01 g/L花青素的联合添加。 相似文献
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氯霉素残留检测阻断ELISA试剂盒的研制及性能测定 总被引：1，自引：0，他引：1
在建立氯霉素单克隆抗体（CAP mAb）杂交瘤细胞株和阻断ELISA方法的基础上．研制出CAP残留快速检测试剂盒（CAP-kit），并对其性能进行了测定。结果表明，CAP-kit的标准曲线呈典型的S型，符合4参数logit曲线拟合，相关系数R^2=0．9953，检测范围为1．0μg/L～128．0μg/L，灵敏度为0．85μg／L，半数抑制浓度（IC50）为7．54μg/L，检测限为1．0μg/L；牛奶样、猪肉样的平均添加回收率为88．3％、82．5％，平均批内和批间变异系数均〈15％；CAP-kit与氯霉素琥珀酸钠的交叉反应（CR％）为150％，与其它酰胺醇类和抗菌素药物无交叉反应；试剂盒在4℃可保存6个月。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献