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目的：比较实验动物专用血细胞分析仪与医用血细胞分析仪对SD大鼠血液生理指标测定值的差异,分析检测仪器对大鼠血液生理指标测定的影响。方法：36只SD大鼠,体重约200 g,雌雄各半,屏障环境饲养,试验前禁食12 h,麻醉后采血,分别用实验动物专用血细胞分析仪与医用血细胞分析仪测定20项血液生理指标。结果：两种仪器分别检测的SD大鼠的平均血红蛋白浓度、平均血红蛋白含量、白细胞数量、淋巴细胞数量、单核细胞数量、嗜碱性粒细胞数量、嗜中性白细胞百分比、单核细胞百分比、嗜酸性粒细胞百分比、嗜碱性粒细胞百分比、淋巴细胞百分比、血小板数量、血小板分布宽度差异极显著（P＜0.01）;血红蛋白浓度、嗜中性白细胞数量、红细胞分布宽度差异显著（P＜0.05）;红细胞数量、红细胞压积、平均红细胞体积、嗜酸性粒细胞数量差异不显著（P＞0.05）。结论：不同检测仪器对SD大鼠血液学指标的检测有影响。 相似文献
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Coulter分析仪在恒河猴血液检验中的应用评价 总被引：1，自引：0，他引：1
为探讨Coulter血液细胞分析仪的性能及其在实验动物血液分析中的应用，对恒河猴静脉血按常规方法、Coulter分析仪分别进行了测定和比较。结果显示，Coulter分析仪具有良好的技术性能，基本能满足对血细胞检查的需要；但对白细胞分类和异常细胞的检测时，该分析仪的作用仍只能是筛选，建议使用显微镜分析。 相似文献
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《中国家禽》2017,(24)
通过分析比较传统方法和血细胞分析仪测定的鸡红细胞总数(RBC)、血红蛋白含量(HGB)和红细胞压积(PCV),探讨能否应用血细胞分析仪代替传统方法采集肉鸡相关血液数据。120只AA商品代肉仔鸡常规饲养,15日龄时随机分成两组。对照组常温(22～24℃)饲养;低温组从15日龄开始以每日1～2℃的速度逐渐降温,到21日龄时,室内温度维持在10～12℃直到试验结束。在28和42日龄时,每组随机抽取12只肉鸡,静脉采血。分别用传统方法和分析仪分别测定RBC、HGB和PCV。结果显示:仪器测定的RBC、HGB不能反映肉鸡血细胞的实际数值,PCV与实际值接近;分别对两种方法测得数据进行方差分析,结果显示组间差异变化相似,两种方法检测结果极显著相关(P0.01)。研究结果表明,全自动血细胞分析仪测定的肉鸡血液RBC、HGB和PCV值在进行组间差异分析时具有借鉴意义。 相似文献
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走出医疗器械管理中存在的日常维护与保养和被动维修的误区,加大对大型贵重医疗设备、仪器的维护与保养力度,延长设备、仪器的使用寿命,提高医院的综合效益. 相似文献
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《北方牧业(奶牛)》2006,(5):50-51
正确的使用方法和良好的维护是所有挤奶设备高效优质工作的基础，也是延长设备使用寿命的必要条件。韦斯伐利亚的挤奶设备在我国大型牧场使用较多，以下就针对如何正确使用和维护韦斯伐利亚的挤奶设备作一详细的介绍。 相似文献
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外墙保温技术既有利于建筑节能又有利于国家经济可持续发展,延长了建筑物使用寿命,节约了能源资源,是建筑发展趋势。我国外墙保温技术发展很快,节能材料的发展必须与外墙保温技术相结合,才能真正发挥其作用,发展外墙保温技术及节能材料使用是建筑节能的主要实现方式。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献