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试验旨在研究myoneurin（MYNN）基因在猪不同组织中的表达特征及其在肌肉（背最长肌、股二头肌和腰大肌）、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。采用实时荧光定量PCR技术研究猪MYNN mRNA在90日龄大白猪和马身猪的心脏、肝脏、脾脏、肺脏、肾脏、小脑、小肠、胰脏、胃、股二头肌及脂肪共11个组织中的表达谱,以及在大白猪和马身猪1、90、180日龄3个发育阶段的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的发育性表达规律。结果表明,MYNN在猪的各种组织中广泛表达,且各组织间表达差异显著或极显著（P < 0.05;P < 0.01）;MYNN在大白猪和马身猪的肌肉、小脑、肝脏、胰脏、肾脏、胃、脾脏、肺脏组织中的不同发育阶段表达差异显著或极显著（P < 0.05;P < 0.01）,并具有特定规律,由此推测其可能在猪的这几种组织中发挥重要作用。MYNN基因的表达与组织、日龄及品种的遗传背景有关。本试验为研究猪MYNN基因的生物学功能提供了依据,但还需要深入的研究来探索其作用的具体机制,尤其是在骨骼肌发育中的调节机制。 相似文献
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采用RT-PCR分析填饲前后朗德鹅11种组织PPAR基因的表达差异性,结果显示,除腹脂外,PPAR-α在其他10种组织中都有表达,其中较高表达于心脏、肝脏、肾脏、十二指肠和胸肌;中等程度表达于肌胃、全脑、腿肌;较低表达于脾脏和肺脏.填饲后发现该基因在肾脏中依然高表达,除肺脏中表达量升高外,在其他组织中表达量均下降.PPAR-γ较高表达于肝脏、脾脏、肾脏、十二指肠和腹脂,较低表达于其他组织.填饲后发现该基因在心脏、脾脏、肺脏、肾脏、肌胃中表达量升高,在肝脏、十二指肠、全脑、胸肌、腿肌中表达量基本保持不变,在腹脂中表达量降低.结果表明PPARs风的表达具有组织特异性,填饲前后的表达量变化反映PPARs基因影响鹅肥肝的形成. 相似文献
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试验分别采集40日龄小体型猪（巴马猪）和大体型猪（大白猪）的心脏、肝脏、脾脏、肺脏、肾脏、头骨、骨骼肌组织,利用实时荧光定量PCR检测斯钙素-1（stanniocalcin 1,STC-1）基因mRNA在各个组织中的表达水平,并通过Western blotting检测STC-1蛋白在各个组织中的分布。实时荧光定量PCR检测结果表明,STC-1基因mRNA在巴马猪和大白猪肺脏、肾脏中相对表达水平较高,在骨骼肌中的表达水平最低;除心脏和骨骼肌外,巴马猪其余各组织中STC-1基因mRNA表达水平均显著高于大白猪（P < 0.05）。Western blotting检测结果表明,巴马猪肝脏中STC-1蛋白的表达量最高,而大白猪脾脏中STC-1蛋白表达量最高,两者差异显著（P < 0.05）;巴马猪肺脏、肝脏、骨骼肌及心脏组织中STC-1蛋白表达量均极显著高于大白猪（P < 0.01）;而巴马猪肾脏、脾脏中STC-1蛋白表达量极显著低于大白猪（P < 0.01）。本研究首次对大、小体型猪不同组织的STC-1基因mRNA表达水平及其STC-1蛋白分布进行检测,导致该基因表达与分布差异的原因可能与两种猪受外界环境应激及生长发育差异有关。 相似文献
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本研究旨在研究猪细小病毒分离株感染初孕母猪后母猪和胎猪的病毒组织分布和组织病理学变化情况。试验选用妊娠35d的初孕母猪接种PPV—BQ株第15代细胞毒,以接种RPMI1640的母猪作为对照。攻毒40d后剖杀,采集母猪的心脏、肝脏、脾脏、肺脏、肾脏、扁桃体、子宫内膜和腹部淋巴结,胎猪的心脏、肝脏、脾脏、肺脏、肾脏、脑和小肠,进行组织病毒载量测定和组织病理学检测。实时定量PCR检测结果显示,攻毒组母猪的心脏、脾脏、肺脏、肾脏和子宫内膜存在病毒复制,子宫内病毒含量最高;胎猪的心脏、脾脏、肺脏中存在病毒复制。对照组脏器中无病毒复制。病理切片结果显示,攻毒组母猪肺脏出现细支气管性肺炎,脾脏出现淋巴细胞减少,心脏、肝脏、肾脏、扁桃体、子宫内膜和腹部淋巴结未见明显的组织病理学变化;胎猪的脏器未见明显的病理损伤。对照组未发现组织病理学变化。 相似文献
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类猪圆环病毒因子P1在自然感染仔猪体内的组织分布 总被引：1，自引：0，他引：1
选用3头自然感染仔猪和1头阴性对照猪,剖杀后采集心脏、肝脏、脾脏、肺脏、肾脏、胰脏、脑、空肠、扁桃体、胸腺、淋巴结、膀胱、性腺等组织,用荧光定量PCR方法检测病毒在组织中的分布及病毒载量.结果显示,类猪圆环病毒因子P1分布在仔猪的心脏、肝脏、肺脏、胰脏、脑和膀胱等组织,病毒载量以胰脏、脑和膀胱为最高,可达105拷贝/g以上；对照猪脏器中没有检测到病毒核酸.该研究为P1的诊断及致病机制奠定了基础. 相似文献
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《黑龙江畜牧兽医》2017,(11)
为了对西藏小型猪GHR基因在不同器官组织和不同年龄阶段的表达情况进行测定,试验采用Real-time PCR的方法,以GAPDH为内参,定量分析0岁(1日龄)、0.1岁(36日龄)、0.25岁(90日龄)、0.5岁(180日龄)、1岁(360日龄)、2岁(720日龄)、3岁(1 080日龄)的西藏小型猪心脏、肝脏、脾脏、肺脏、肾脏、肌肉、皮肤器官组织中GHR基因mRNA表达水平,并且进行比较分析。结果表明:生长激素受体基因在0,0.1,0.25,0.5,1,2,3岁各年龄阶段的心脏、肝脏、脾脏、肺脏、肾脏、肌肉、皮肤各器官组织中均有表达。在不同器官的表达中,GHR基因在1岁、2岁肺脏组织中的表达量最高,肌肉组织中的表达量最低;在0.25岁肾脏组织中的表达量最高,肌肉组织中的表达量最低。在不同年龄阶段的表达中,生长激素受体基因在0.1岁时表达量达到峰值。说明西藏小型猪的生长激素受体基因的表达呈现出明显的时空特异性。 相似文献
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PPAR-γ在八眉猪不同组织中的表达差异 总被引：2，自引：0，他引：2
以八眉猪为试验材料,提取其内脏脂肪、皮下脂肪、肌肉、肾脏、肝脏、心脏、肺脏和脾脏总RNA和总蛋白质,设计合成PPAR-γ1和PPAR-γ2基因的引物,以猪β-actin基因为内参,用半定量（Semi-Quantitative,SQ）RT-PCR检测PPAR-γ1和PPAR-γ2 mRNA在以上各组织中的表达;用免疫印迹技术（Western Blot）检测以上各组织中PPAR-γ1和PPAR-γ2蛋白的表达。结果显示,PPAR-γ1 mRNA及蛋白在以上各组织均表达,在内脏脂肪和皮下脂肪组织中表达水平显著高于其他组织,在肺脏中表达水平最低;PPAR-γ2 mRNA和蛋白质高表达于内脏脂肪、皮下脂肪、脾脏和心脏,而在肺脏、肾脏、肝脏、肌肉中表达水平很低。结果提示,PPAR-γ在不同组织的表达差异,可能与其在不同组织中的功能有关。 相似文献
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本实验以长白猪背最长肌为实验材料,进行组织RNA的提取及A-FABP基因的克隆,构建p EGFPN1-A-FABP真核表达载体,并通过脂质体转染成纤维细胞,48 h观察荧光表达。同时应用荧光定量PCR法检测猪7种不同组织(心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌)中A-FABP基因的差异表达。结果表明:成功构建p EGFP-N1-A-FABP融合表达载体,并在细胞中表达绿色荧光蛋白;A-FABP基因在7种组织中均有表达,其中背最长肌和腿肌中A-FABP基因的表达量最高,与其他组织相比差异极显著(P0.01),而脾脏和肾脏中的表达量相对于心脏、肝脏和肺脏差异显著(P0.05),而A-FABP基因表达量在心脏、肝脏和肺脏中差异不显著(P0.05)。表明该基因能够在真核载体和细胞中表达,并且在猪不同组织的表达具有差异性。 相似文献
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Gross Osteology,Radiographic and Computed Tomographic Morphology of the Axial Skeleton of the Nine‐Banded Armadillo (Dasypus novemcinctus) 
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下载免费PDF全文 L. S. Alves M. Midon A. L. Filadelpho L. C. Vulcano 《Anatomia, histologia, embryologia》2017,46(2):162-177
This study used 20 nine‐banded armadillo, four in vivo and 16 cadavers, to describe the osteoarticular anatomy of the axial skeleton by means of digital radiography and computed tomography. Vertebral formula obtained in this sample specimens was seven cervical, 10 thoracic, five lumbar, nine vertebrae related to the synsacrum and 20–27 free caudal vertebrae. Peculiar features of this species were noted as the presence of xenarthrous processes in the caudal thoracic vertebra to the last lumbar vertebra, with prominent mammillary processes in the thoracolumbar segment, fused cervical vertebra from the second to fourth vertebra and the presence of synsacrum related to sacral and caudal vertebra fused to the pelvis. They are homodont animals presenting only molariform teeth with formula of 8/8, totalling 32 teeth. There was no complexity in the execution of radiographic and tomographic examinations, concluding that execution can be carried out in nine‐banded armadillos during the clinical routine for wild animals. 相似文献
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本研究利用PCR和免疫组化等方法,对FSHR和FSH基因在鸡多个非繁殖系统的组织器官中的mRNA和蛋白水平的表达以及相关性进行了分析.结果显示:在心脏、肝脏、肾脏、肺和十二指肠中FSHR mRNA和蛋白以及FSH蛋白水平具有相同的表达趋势,表达丰度从大到小依次为肝脏、心脏、肾脏、肺和十二指肠.而在脾脏、胃、腿肌和胸肌中未检测到FSHR mRNA和蛋白以及FSH蛋白水平的表达;在检测的9个组织中,均无FSHmRNA的表达.相关性分析结果表明,在肝脏、心脏、肾脏、肺和十二指肠中各组织器官质量与组织中的FSH蛋白水平具有显著或极显著的正相关(P＜0.05或P＜0.01);各组织中的FSHR mRNA和蛋白水平均与FSH蛋白水平存在显著或极显著正相关(P＜0.05或P＜0.01).本研究初步证明,机体其他部位表达分泌的促卵泡素,在多个非繁殖组织器官(肝脏、心脏、肾脏、肺和十二指肠)中通过调控促卵泡素受体基因的表达来影响北京油鸡不同组织的发育. 相似文献
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A nine-year-old neutered male domestic shorthaired cat with a history of spinal pain and progressive hindlimb dysfunction was presented to the Norwegian School of Veterinary Science. Following neurological and myelographic examination, an intramedullary mass affecting several lumbar spinal cord segments was diagnosed. A neoplastic lesion was suspected and a poor prognosis was given. On postmortem examination, the spinal cord was found to be dorsally flattened from the 12th thoracic vertebra to the fifth lumbar vertebra and severely thickened with a dorsal cleft from the fifth to the seventh lumbar vertebra. Histologically, the tumour was diagnosed as an anaplastic astrocytoma. 相似文献
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Kim CS Koh PO Cho JH Park OS Cho KW Kim GS Won CK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):653-655
A male deformed Korean native calf was examined macroscopically. The deformed calf had no caudal vertebral columns from 5th lumbar vertebra, sacrum and coccygeal vertebrae. The spinal cord was terminated in the vertebral foramen of the 3rd lumbar vertebra. The cervical vertebrae had scoliosis and the 3rd and 4th cervical vertebrae were fused. The 2nd and 3rd lumbar vertebrae were fused and the left and right transverse processes of the 4th lumbar vertebra articulated with ala of the ilium. The rectum was greatly expanded by the imperforate anus and a rectourethral fistula was formed between the rectum and urethra. The deformed calf was recorded as a first documentation of sacrocaudal agenesis confirmed in a Korean native calf. 相似文献
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Baek IJ Yon JM Lee SR Kim MR Hong JT Lee BJ Yun YW Nam SY 《Anatomia, histologia, embryologia》2011,40(3):210-218
Gastrointestinal glutathione peroxidase (GI-GPx) is an antioxidant enzyme that has been known to be restricted to the gastrointestinal tract in rodents. In an effort to determine the expression pattern of GI-GPx mRNA during organogenesis, quantitative real-time PCR and in situ hybridization for GI-GPx mRNA were conducted in whole embryos or each developing organ of mice. GI-GPx mRNA was expressed more abundantly in the extraembryonic tissues, including placenta than in embryos on embryonic days (EDs) 7.5-18.5 (P < 0.05). When compared with the expression levels of cytosolic GPx (cGPx) mRNA, GI-GPx mRNA levels were low in the embryos, but relatively high in the extraembryonic tissues (P < 0.05). According to the results of whole mount in situ hybridizations, GI-GPx mRNA was principally expressed in the ectoplacental cone, neural tube and fold, and primitive heart at EDs 7.5-8.5. At EDs 9.5-12.5, GI-GPx mRNA was abundantly expressed in nervous tissues such as the telencephalon, mesencephalon and dorsal neural tube and was also detected in the forelimb and hindlimb at EDs 10.5-12.5. In the sectioned embryos after ED 13.5, GI-GPx mRNA levels were high in the cerebral cortex, metanephric corpuscle, pancreatic ducts, surface epithelia of the skin, inner ear, and nasal conchae, gastrointestinal tract, liver, urinary bladder, airway passages of lung, and whisker follicles. These findings indicate that GI-GPx is not only spatiotemporally expressed in a variety of embryonic organs during organogenesis but also may perform a mutual compensatory role with the cGPx in the protection of embryos and extraembryonic tissues against the reactive oxygen species generated in ontogenetic periods. 相似文献
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Letícia Rocha Inamassu Bruno Cesar Schimming Carmel Rezende Dadalto Maria Jaqueline Mamprim 《Anatomia, histologia, embryologia》2021,50(1):128-135
The white-eared opossum present a higher roadkill number in Brazil, and the vertebral column is often affected when some type of trauma occurs. Thus, this study describes the osteology and radiology of the vertebral column in white-eared opossum to serve as a basis for support in the veterinary clinical care, since the casuistry of care of this marsupial has increased every year. Radiographs of the cervical (C), thoracic (T), lumbar (L), sacral (S) and caudal (Cd) segments of the vertebral column were taken in thirty-five white-eared opossums (Didelphis albiventris). Furthermore, ten opossums without evident vertebral acquired or congenital abnormalities at the radiographic study were selected for anatomical study. Osteology was correlated with the radiographic images. The use of radiographs allowed the identification of congenital and acquired vertebral changes, as well as the identification of anatomical structures, their distribution and morphology and the number of vertebrae. The vertebral formula varied in the white-eared opossum. In 27 of 35 white-eared opossums (77.2%) with normal vertebral morphology, three vertebral formulas were observed: C7/T13/L6/S2 (62.9%), C7/T13/L5/S2 (5.7%) and C7/T13/L7/S2 (8.6%). Most of the specimens presented 27 caudal vertebrae. The anticlinal vertebra was identified as T10 in 77.2% and T11 in 22.8% of opossums. Cervical block vertebra was observed in two animals and sacrocaudal block vertebra in one animal. The haemal arches could be seen from Cd3–Cd4 in 80% and Cd2–Cd3 in 20% of opossums. The white-eared opossum showed a slender vertebral column with a relatively short thoracic region, a relatively long lumbar region and a highly flexible lumbosacral transition, suggesting that this animal is a fast-running and agile opossum. Therefore, these findings may serve as a basis for further research and support the veterinary clinical care, since the casuistry of care for this marsupial has increased every year. 相似文献
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以成年母猪为研究材料,采用RT-PCR半定量法对猪PPARα、β/δ和γ基因组织表达特点进行了研究。结果表明:在检测的18种组织中除胰腺组织外,3种PPAR亚型在其他17种组织中均有表达。表达量高低依次为PPARα,子宫绒毛膜>皮下脂肪>卵巢>大肠>脾脏>大脑>肾上腺>心脏>肺>小肠>脊髓>子宫蜕膜>胃>肝脏>背最长肌>膀胱>肾脏;PPARδ,子宫绒毛膜>卵巢>大肠>脾脏>肝脏>胃>皮下脂肪>大脑>肺>肾上腺>子宫蜕膜>脊髓>背最长肌>心脏>肾脏>小肠>膀胱;PPARγ,背最长肌>皮下脂肪>卵巢>脾脏>肺>大肠>膀胱>子宫绒毛膜>子宫蜕膜>心脏>胃>肝脏>肾脏>大脑>脊髓>肾上腺>小肠。3种亚型PPAR在卵巢和/或子宫绒毛膜中都有较高的表达,提示它们与猪的繁殖性能相关。 相似文献
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Takanori Ueno Shinya Wada Seigo Mashita Atsutoshi Kuwano Yoshinari Katayama 《Journal of Equine Veterinary Science》2012
A 15-year-old Dutch Warmblood gelding suddenly developed incoordination and hindlimb stumbling. The horse had a history of eyelid lymphoma. Necropsy revealed yellow-white or dark reddish-brown masses adhering to the outer surface of the spinal dura mater from the first cervical vertebra to the seventh thoracic vertebra. The spinal cord close to the first cervical vertebra and the seventh thoracic vertebra was markedly compressed by the masses filling the epidural space. The masses were also observed in the larynx, eyelids, and adipose-rich tissues, including the joints and orbits. They appeared similar in shape. The mandibular, retropharyngeal, axillary, superficial inguinal, deep inguinal, and lateral iliac lymph nodes were solid and enlarged. Histologically, the masses were composed of small or medium-sized lymphocyte-like tumor cells, but atypical cells and mitotic figures were rare. There were moderate infiltrations of macrophages and multinucleated giant cells, which were occasionally ingesting the surrounding tumor cells. Immunohistochemically, the tumor cells were classified as T-cell-derived cells. Throughout the spinal cord, enlargement or loss of nerve axons, dilation of periaxonal spaces, and macrophage infiltration into periaxonal spaces were observed, mainly in the ventral funiculus. Spinal cord compression by the tumor mass was suggested as a cause of the locomotive dysfunction. This is the first report of equine lymphoma with ataxia located from the proximal cervical to middle thoracic dura mater and in joint cavities. 相似文献
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Johnston PE Barrie JA McCulloch MC Anderson TJ Griffiths IR 《The Veterinary record》2000,146(22):629-633
The neuropathology of 20 German shepherd dogs and five German shepherd dog crosses with chronic degenerative radiculomyelopathy were analysed by conventional techniques, immunocytochemistry and electron microscopy. There were previously unrecognised changes in brain nuclei. In the spinal cord, both motor and sensory tracts were involved, principally in their more distal regions. Wallerian degeneration affected the corticorubrospinal pathways in the lateral columns and the ventral funiculi, predominantly in the caudal thoracic and lumbar segments, although more cranial involvement was also observed. The dorsal columns were affected in the caudal lumbar region and the cervical fasciculus gracilis. The regional distribution was variable between cases. Within the brain, abnormalities, including chromatolysis, gliosis and neuronal loss were observed in the red nucleus, lateral vestibular nucleus and, occasionally, in the dentate nucleus. The changes in brain nuclei were compared with those found in dogs at various times after a focal spinal injury. The neuronal changes in the brain may be related to the primary site of damage, and possible aetiological mechanisms are discussed. 相似文献
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Polizopoulou ZS Kazakos G Patsikas MN Roubies N 《Journal of Feline Medicine and Surgery》2005,7(6):363-368
A case of hypervitaminosis A with secondary entrapment and compression of the left brachial plexus nerve roots is described. A 9-year-old male castrated domestic shorthair, fed a home-made diet based on raw pork liver, was submitted for examination for a left forelimb lameness that evolved to paralysis over a 2-month period. Clinical examination revealed a flaccid paralysis and atrophy of all left forelimb muscles. An ipsilateral Horner's syndrome was also noted. Radiological examination of the cervical and thoracic spine showed massive new bone formation at the ventral aspect of the second cervical to sixth thoracic vertebra. The diagnosis of hypervitaminosis A was made, based on the clinical and radiographic findings, as well as the determination of serum vitamin A concentration, which was 630 microg/dl, three times above the upper normal limit for this species. Despite the unfavourable initial prognosis, the cat progressively regained function of the affected limb approximately 6 months after the diet was changed to a commercial canned food. 相似文献