共查询到20条相似文献，搜索用时 140 毫秒
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鱼卵和胚胎冷冻保存研究进展 总被引：3，自引：1，他引：3
<正> 一、前言自Polge等(1949)成功地用甘油冷冻保存动物精子以来,低温生物学的原理很快被应用于动物卵子和胚胎的冷冻保存。目前,冷冻配子和胚胎已在医学和畜牧业中得到广泛应用。低温生物学在畜牧业生产和种群管理中的成功应用极大地鼓舞了鱼类生理学者们,并使其对鱼类卵子和胚胎的低温保存进行实验研究。由于鱼类卵子体积很大,具有二层 相似文献
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鱼类精液超低温冷冻保存的基本原理、研究现状及应用前景 总被引：6，自引：1，他引：6
<正> 利用低温保存生命是一项新兴的科学手段。自1949年英国Polge用甘油冷冻保存动物精液成功以来,这项技术突飞猛进。目前冷冻配子及胚胎已经应用在医学研究和畜牧业生产上。而水产生物配子(主要是精子)的冷冻保存,经过三十年的努力也取得很大成就。从现有资料看,鱼类精液冷冻保存的研究大多集中在冷水性的鲑鳟鱼类以及某些海水鱼类上,结果较为理想。而有关鲤科鱼类精液冷冻保存的研究报道尚不多。冷冻配子具有超越时空的功效,可以使不同生殖期或地理间隔的品系得以交配,性转换的个体得以自交;可以不间断地为水产遗传学及生物技术研究提供配子材料;还可 相似文献
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鱼类、水生生物配子及胚胎低温冷冻保存研究进展 总被引：8，自引：1，他引：8
自从五十年代初Blaxter开始大西洋鲜鱼精巢短期冷冻保存研究以来，国外的鱼类精子超低温冷冻保存研究已取得了很大进展，正在从最初的基础理论研究和方法探索向实用技术研究转变。但是，鱼类的卵子和胚胎研究进展就远不如精子。目前绝大部分工作都停留在解决冷冻过程中各技术环节的处理及冷冻机理的研究阶段。许多学者为了解决这些问题，把研究方向转向了体积较小的各种水生生物配子，以求从中找出解决问题的办法。我们通过计算机检索，对所查到的文献资料（特别是1090年之后的资料）进行了系统的归类整理，拟就国外在这方面所取得的最新研… 相似文献
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玻璃化液作为一种比较理想的低温保护剂,对鼠胚胎和牛胚胎保存已获成功,但对适合于鱼类胚胎保存的至今未看到报道.为了研究玻璃化液对鱼类胚胎的低温保存效果,使鱼类胚胎低温冷冻保存获得成功,作者开展了利用不同的低温保护剂,采用不同浓度的组合,经超低温冷冻筛选后找出了能形成玻璃化的最低浓度,并利用这些能形成玻璃化的抗冻剂对泥鳅胚胎进行了毒性实验(1998),但还没有找出对胚胎保存效果好、毒性小、"高渗”损伤小的最佳玻璃化液.本文进一步利用这些玻璃化液对在不同条件下,对不同发育时期的泥鳅胚胎延长玻璃化对胚胎的作用时间,进行了系统的研究,现将结果总结如下. 1 材料和方法 相似文献
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玻璃化液对泥鳅胚胎成活率的影响 总被引：2，自引：0，他引：2
玻璃化液作为一种比较理想的低温保护剂 ,对鼠胚胎和牛胚胎保存已获成功 ,但对适合于鱼类胚胎保存的至今未看到报道。为了研究玻璃化液对鱼类胚胎的低温保存效果 ,使鱼类胚胎低温冷冻保存获得成功 ,作者开展了利用不同的低温保护剂 ,采用不同浓度的组合 ,经超低温冷冻筛选后找出了能形成玻璃化的最低浓度 ,并利用这些能形成玻璃化的抗冻剂对泥鳅胚胎进行了毒性实验 (1998) ,但还没有找出对胚胎保存效果好、毒性小、“高渗”损伤小的最佳玻璃化液。本文进一步利用这些玻璃化液对在不同条件下 ,对不同发育时期的泥鳅胚胎延长玻璃化对胚胎的作… 相似文献
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利用显微注射技术冷冻保存牙鲆胚胎 总被引：1，自引：0，他引：1
在鱼类胚胎的冷冻保存中,需要抗冻剂有效地渗入胚胎,才能对胚胎起到保护作用.本研究采用显微注射技术将抗冻剂直接注射到牙鲆(Paralichthys olivaceus)胚胎内,以实现牙鲆胚胎的玻璃化低温冷冻保存.研究中,首先对抗冻剂种类进行了选择,将抗冻剂的毒性由大到小依次排列为:乙二醇(EG)、甘油(Gly)、二甲基亚砜(DMSO)、甲醇(MeOH)、1,2丙二醇(PG)、PM21(PG:MeOH=2:1);对胚胎发育时期和注射剂量进行了筛选.实验结果显示,注射600pL(1 pL=10-6mL)抗冻剂PM21后,牙鲆的心跳期胚胎成活率显著高于尾芽期胚胎(P＜0.05),成活率为(64.04 2.05)%;对卵黄囊、卵膜与卵黄膜间隙作为注射部位进行了选择,发现采用卵黄囊内注射的胚胎成活率高于"五步平衡法",并显著高于通过卵黄膜间隙注射(P＜0.05),其成活率为(44.24±7.88)%.结果表明,注射600pL 35%PM21至牙鲆心跳期胚胎卵黄囊内,平衡10min,然后进行玻璃化冷冻保存,取得了68.2y.4%透明胚胎,能够对牙鲆胚胎提供很好的保护.说明显微注射方法可以成功地将抗冻剂注射入牙鲆胚胎,并在牙鲆胚胎的冷冻及胚胎的完整性方面发挥有效的保护作用. 相似文献
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鱼类配子和胚胎冷冻保存研究进展及前景展望 总被引：25，自引：5，他引：25
种质资源是水产养殖生产、优良品种培育及水产养殖业可持续发展的重要物质基础。我国是一个水生生物种质资源较为丰富的国家 ,丰富多样的水生生物种质资源和遗传多样性对于我国水产养殖业的快速发展起到了非常重要的作用。然而 ,由于渔业资源的过度捕捞、无序利用及人工放流等 ,造成了某些鱼类资源的衰退和濒临灭绝 ,如不及时采取保护措施 ,若干年后 ,在自然界中将难以找到上述鱼类原种、良种的遗传资源 ;由于忽视鱼类种质保护及品种选育的工作 ,养殖鱼类近亲交配越来越严重 ,造成种质退化 ,遗传多样性减少 ,生长速度减缓 ,品质下降 ,对病害… 相似文献
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Evaluation of the potential source of bacterial contamination during cryopreservation process of silver barb (Barbodes gonionotus) sperm 
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下载免费PDF全文 Traimat Boonthai Weerasith Khaopong Jumlong Sangsong Treerat Sooksawat Verapong Vuthiphandchai Subuntith Nimrat 《Aquaculture Research》2016,47(7):2101-2113
Application of good sanitation practice in cryopreservation process is the key issue to improve the quality of cryopreserved fish sperm. This study implemented standard sanitation protocol with minimal contamination and evaluated the source of bacterial contamination associated with laboratory equipment and related materials across a series of cryopreservation process of silver barb (Barbodes gonionotus) semen. The use of 16s rRNA sequencing and traditional biochemical methods were performed for bacterial identification. Animal origin (anal fin, culture water and semen contaminated with faeces and urine) and non‐animal origin (liquid nitrogen from liquid nitrogen dewar, outer surface of straw, air circulation in cryopreservation laboratory and latex gloves used during cryopreservation procedure) were determined for bacterial contamination. Aeromonas punctata subsp. caviae was the most abundant species in anal fin, latex groves and semen contaminated with faeces and urine. Bacillus safensis and Bacillus sp. were found as frequently recovered species from liquid nitrogen dewar. Aeromonas hydrophila subsp. hydrophila and Pseudomonas fluorescens, fish pathogenic bacteria, were isolated from all animal origin samples. This was the first report indicating that standard sanitation and hygiene methodologies are recommended for sperm cryopreservation of B. gonionotus to prevent bacterial contamination. 相似文献
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Jianlin Pan Shuyan Ding Jiachun Ge Weihui Yan Chen Hao Jianxiu Chen Yahong Huang 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):173-176
Yellow catfish (Pelteobagrus fulvidraco) is a candidate freshwater fish for aquaculture in China with its high consumer demand. The aim of this study was to examine the possibility of storage of the sperm of yellow catfish by cryopreservation in liquid nitrogen. Experiments were designed to investigate the effects of the different combinations of three extenders (Ringer extender, Kurokura-1 extender and D-15 extender) and three cryoprotectants (DMSO, Glycerol and Methanol) on the cryopreservation of yellow catfish sperm. Post-thaw sperm motility, fertilization and hatching rate were detected to evaluate the reliability of sperm cryopreservation. The results demonstrated that Ringer extender and 10% methanol was the best combination for protecting the sperm during freezing in liquid nitrogen by a three-step method and thawing in a water bath at 37 °C for 60 s. In this combination for cryopreservation, sperm maintained the highest post-thaw motility (65 ± 5%), fertilization (90.47 ± 3.67%) and hatching rate (88 ± 4%). And more interestingly, the fertilization and hatching rate were similar to those of fresh sperm (97.55 ± 2.74% and 92 ± 5%). Successful sperm cryopreservation techniques for yellow catfish have been developed for hatchery purpose. 相似文献
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The cryopreservation of semen from the Northern pike, Esox lucius L., was investigated with a method that was originally developed for the Salmonidae. Because the amounts of semen obtained by stripping were insufficient, the suitability of testicular sperm was tested for cryopreservation. Frozen-thawed testicular sperm had fertilization rates similar to frozen-thawed semen obtained by stripping (74.2-84.7%), and at sperm to egg ratios of S= 4.5 × 105 spermatozoa per egg, the post-thaw fertilization rates were also similar to fresh, untreated semen controls. Out of all the fertilization solutions investigated, a 100-mm NaCl, 10-mm Tris (pH 9) solution resulted in the highest post-thaw fertilization rates. To facilitate the fertilization of large egg batches, 1.2-mL straws were used for cryopreservation with a similar efficiency to 0.5-mL straws. 相似文献
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超低温冷冻对斑尾刺虾虎鱼卵中酶活性的影响 总被引：1，自引：0，他引：1
研究了超低温冷冻保存(-196℃)对斑尾刺虾虎鱼成熟卵中总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)等酶活性的影响。结果表明,经过冷冻保存后,总ATP酶、CK、SDH和LDH的活性显著下降(P<0.05),同时可见添加抗冻剂组比未添加抗冻剂组下降幅度更大(P<0.05);SOD、CAT和GSH-PX的活性也显著下降(P<0.05),未添加抗冻剂组比添加抗冻剂组下降更明显(P<0.05);MDA的活性显著升高,且未添加抗冻剂组显著高于添加抗冻剂组(P<0.05)。超低温冷冻和是否添加抗冻剂都对斑尾刺虾虎鱼卵中酶活性有显著性影响。 相似文献
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为筛选出更有效的虹鳟(Oncorhynchus mykiss)精液冷冻保存液,在以10％ 甲醇和0.3 M葡萄糖作为主要成分的基础上,分别或同时添加0.9 g/L氯化钾和10％ 蛋黄形成四种冷冻保存液,用于虹鳟精液冷冻保存试验,分别形成4个处理组:Ⅰ#(不添加氯化钾和蛋黄)、Ⅱ#(添加氯化钾)、Ⅲ#(添加蛋黄)和Ⅳ#(... 相似文献
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Teruyoshi Narita Takayuki Kawamoto Kiyoshi Isowa Hideo Aoki Masahiro Hayashi Hiromi Ohta Akira Komaru 《Fisheries Science》2008,74(5):1069-1074
To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular
spermatozoa were diluted with cryopreservation diluent (10% methanol+18% fetal bovine serum+72% sea water), and dispensed
into 0.25-mL straws. The straws were cooled at a rate of approximately −20 °C/min to −50°C, and subsequently immersed in liquid
nitrogen. Percentage motility of spermatozoa before cryopreservation was 69.9±4.2%, and that of cryopreserved spermatozoa
was 24.0±1.8%, respectively. In cryopreserved spermatozoa, the percentage that lacked or had a deformed flagellum was 56.6±3.9%,
while in fresh spermatozoa this was 8.7±2.0%. In cryopreserved spermatozoa, the percentage of deformed acrosomes was 76.6±5.2%,
while in fresh spermatozoa this was only 0.9±0.3%. Cryopreserved spermatozoa with a normal acrosome and flagellum were only
15.4±3.5% of those in fresh spermatozoa. These results indicate that lesion of the flagellum and deformation of the acrosome
occurred through the cryopreservation procedure, and both types of damage lead to loss of the motility and fertility in thawed
spermatozoa. 相似文献
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