共查询到20条相似文献，搜索用时 593 毫秒
1.
以H5N1亚型虎源流感病毒免疫Balb/c小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0融合,用间接ELISA试验检测细胞培养上清,获得2株阳性杂交瘤细胞克隆株,命名为3A13和1B8.分别制备腹水后进行纯化,获得了抗H5亚型虎源流感病毒血凝素的单克隆抗体,用制备的单克隆抗体结合胶体金免疫层析技术,制备了H5亚型虎源流感病毒免疫胶体金快速诊断试纸条.该试纸条对H5N1虎源流感病毒鸡胚培养毒、H5亚型禽流感病毒标准抗原,以及24份虎疑似感染H5N1亚型虎源流感病毒感染病料和小鼠模型病料等检测结果为阳性,同时对H7和H9亚型禽流感病毒标准抗原及传染性支气管炎、新城疫抗原等检测结果为阴性;与HA-HI和RT-PCR的平行对比试验表明,试纸条检测敏感度与血凝试验和血凝抑制试验的敏感性相符.本试验所研制的免疫胶体金诊断试纸条可用于H5亚型虎源流感病毒的特异诊断和流行病学调查,为开发成品化检测试纸条奠定基础. 相似文献
2.
《养禽与禽病防治》2017,(8)
为了验证荧光免疫层析法检测H7亚型禽流感病毒的可行性,采用荧光免疫层析法分别对标准抗原和临床样品进行检测,评价该方法的特异性、敏感性、稳定性和方法的适用性。结果为H7亚型流感病毒荧光免疫层析试纸条能够检出H7N9、H7N2亚型禽流感抗原阳性,对其他抗原检测结果为阴性;能检出H7N9、H7N2亚型禽流感病毒抗原(血凝效价1:512)1 000倍的稀释度;检测10次H7N9亚型禽流感抗原的变异系数为1.12%,差异不显著;检测临床100份样品结果均为阴性。结果表明,应用荧光免疫层析法检测H7亚型禽流感病毒操作简便、试验时间短、不受人为因素干扰,适合市场、养殖场大规模的监测或检疫。 相似文献
3.
蛋白A法压电免疫传感器检测H9亚型禽流感病毒的研究 总被引：1，自引：0，他引：1
为建立可以检测H9N2亚型流感病毒的蛋白A法免疫传感器,将石英晶片谐振的高灵敏度与免疫反应的特异性相结合,用蛋白A(SPA)在镀金膜的石英晶体电极偶联抗H9亚型流感病毒的单抗构建传感器探头形成免疫传感器.检测结果表明,该方法具有较好的特异性,不与H5亚型禽流感病毒和NDV反应;检测灵敏度达到20个EID50;组成的检测器对相应的流感病毒响应良好,该法与鸡胚病毒分离法检出流感病毒的符合率达到91%.本文结果为检测流感病毒免疫传感器的进一步深入研究奠定了基础. 相似文献
4.
为建立一种简便、快速、灵敏、准确的禽流感病毒N9亚型检测方法,根据GenBank中收录的禽流感病毒N9亚型高度保守的基因序列设计引物,通过优化反应条件建立禽流感病毒N9亚型RT-PCR检测方法,并对该方法进行灵敏度、敏感性、特异性试验和临床样品检测。建立了禽流感病毒N9亚型RTPCR检测方法,用该方法检测时,H7N9、H10N9均呈阳性,而非N9亚型的禽流感病毒及新城疫病毒等其他禽传染病病毒均呈阴性,能检测到1/32血凝单位的H7N9禽流感病毒。建立了禽流感病毒N9亚型RTPCR检测方法,具有操作简便、灵敏度高、特异性强的特点,值得推广应用。 相似文献
5.
从发病蛋鸡群分离到H9N2亚型禽流感病毒TJ1株,对其进行了系统的生物学特性鉴定.结果表明,分离毒TJ1株具有血凝性,且凝集不被新城疫和产蛋下降综合征抗血清抑制,而被H9亚型禽流感病毒标准阳性血清抑制,禽流感琼扩试验结果为阳性,在电镜下呈典型的流感病毒粒子形态,证明该毒株为A型流感病毒;通过致病力试验,分离毒TJ1株对6周龄SPF鸡无致病性,为低致病性毒株;用其制备成油乳剂灭活疫苗免疫雏鸡,能很快产生对H9特异的血凝抑制抗体,于免疫后3周攻毒,可以保护雏鸡抵御同病毒攻击的感染,表明分离毒TJ1株具有良好的免疫原性. 相似文献
6.
具有HI活性的抗H5亚型流感病毒特异性单克隆抗体的研制 总被引：1，自引：2，他引：1
以H5N1亚型禽流感病毒分离株A/Duck/Zhejiang/11/00(H5N1)(DZJ/1100)作为免疫原,浓缩纯化后免疫BALB/c小鼠,三次免疫后,取免疫小鼠脾细胞与SP2/O细胞融合,融合阳性细胞用血凝抑制(HI)方法进行检测,阳性细胞株经三代克隆纯化后,获得三株能稳定分泌抗血凝素特异性HI单克隆抗体的杂交瘤细胞株,分别命名为DD7、FG10、CG12.三株杂交瘤细胞株产生的小鼠腹水和细胞培养液上清的HI滴度分别是213、215、211和27、8、23.与其他具有血凝活性的禽类病毒以及其他14个HA亚型的禽流感病毒的交叉HI试验表明:这三株单抗具有良好的禽流感病毒亚型特异性;与其他H5亚型流感病毒分离株的HI试验和中和试验证实这三株单抗具有良好的交叉性(分别为7/8、8/8、8/8)和中和活性(6/8、8/8、8/8).该亚型特异性单克隆抗体的研制成功为H5亚型禽流感病毒的疫情病原学快速诊断提供了坚实的物质保障. 相似文献
7.
基于表面增强拉曼散射(surface enhanced Raman scattering, SERS)和适配体捕获相结合技术，建立1种H5N1亚型禽流感病毒快速检测方法。本实验室筛选得到2条针对H5N1亚型禽流感病毒具有良好的特异性和灵敏度的适配体，与磁珠偶联建立针对H5N1亚型禽流感病毒捕获试剂，通过对捕获体系进行原位还原生成纳米银颗粒增强拉曼信号，建立了原位、快速、无损的H5N1亚型禽流感病毒拉曼光谱快速检测技术。结果表明：H5N1亚型禽流感病毒在1 058 cm-1处有特异性拉曼峰谱，该方法具有良好的特异性、灵敏性和稳定性。该方法的最低检测限达到原病毒液稀释的1010倍。利用本方法可在10 min内快速检测出待测样本中是否有H5N1亚型禽流感病毒的存在，能够在大体积、低浓度样品中快速检测出目标病原，满足H5N1亚型禽流感病毒临场快检的需求。 相似文献
8.
田启会 《国外畜牧学(猪与禽)》2019,39(6):58-59
为了防止禽流感的大规模暴发,减轻对人类健康的影响,本试验采用荧光免疫层析法对某地区养殖户的100羽患病鸡进行诊断。结果显示,通过H5亚型流感病毒荧光免疫层析试纸条检测,该地区的100羽疑似患病鸡样本中,H5N1、H5N2亚型禽流感抗原检出阳性,而H9N2亚型禽流感抗原、H6N6亚型禽流感抗原结果为阴性;能检出H5N1、H5N2亚型禽流感病毒抗原100倍的稀释度;10次检测H5N1亚型禽流感抗原的变异系数为0.95%(P<0.05),差异不显著。此次诊断表明,该地区存在H5N1和H5N2型禽流感,不存在其他血清型的禽流感。 相似文献
9.
通过血凝抑制(HI)和鸡胚中和试验(VN)证实，本实验室制备的抗禽流感H9M2单抗11A5和11B2株可特异性地抑制H9亚型禽流感病毒的血凝特性，而与禽流感H5和H7亚型以及其他具有血凝性感染禽类的病毒(如新城疫等)不反应。两株单抗腹水HI效价均达到15Log2。中和试验表明：上述两株单抗均可有效抑制H9N2病毒在SPF鸡胚中的增殖，使病毒失去血凝活性．测得11A5和11B2株腹水对H9N2禽流感病毒的半数保护量分别为10^-3.35和10^-4.58。该单抗的研制成功对于进一步建立快速鉴别诊断禽流感H9亚型病毒具有重要意义。 相似文献
10.
《上海畜牧兽医通讯》2017,(3)
为研制H5N6亚型流感病毒核酸快速检测试剂盒,选择H5N6亚型流感毒株序列相对最保守的HA基因和NA基因作为H5N6检测的靶序列,设计两套特异性的引物和探针,建立基于TaqMan探针的双重荧光RT-PCR快速检测H5N6亚型禽流感病毒的方法。实验结果表明,所建立的H5N6亚型流感病毒real time RT-PCR检测方法 HA基因检测的灵敏度为10~(-5),NA基因检测的灵敏度为10~(-4),重复性良好,特异性佳。结论:本研究建立的双重荧光定量RT-PCR技术可以快速、准确地检测H5N6亚型流感病毒。 相似文献
11.
12.
13.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
16.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
18.
19.
20.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献