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1.
Cadmium (Cd) is a non-essential element and toxic to plants. To investigate the genetics of Cd tolerance and accumulation
in rice, quantitative trait loci (QTL) associated with Cd tolerance and accumulation at the seedling stage were mapped using
a doubled haploid (DH) population derived from a cross between a japonica JX17 and an indica ZYQ8. A total of 22 QTLs were found to be associated with shoot height (SH), root length (RL), shoot dry weight (SDW), root
dry weight (RDW), total dry weight (TDW) and chlorophyll content (CC), and 10 and 12 QTLs were identified under the control
and Cd stress conditions, respectively. For Cd tolerant coefficient (CTC), 6 QTLs were detected on chromosomes 1, 3, 5, 8
and 10. Under Cd stress, 3 QTLs controlling root and shoot Cd concentrations were mapped on chromosome 6 and 7. One QTL for
shoot/root rate of Cd concentration was identified on chromosome 3. The results indicated that Cd tolerance and accumulation
were quantitatively inherited, and the detected QTLs may be useful for marker-assistant selection (MAS) and identification
of the genes controlling Cd tolerance and accumulation in rice. 相似文献
2.
QTL analysis of seed storability in rice 总被引：7，自引：0，他引：7
A double haploid population, which consists of 127 lines derived from anther culture of a typical indica and japonica hybrid ‘ZYQ8’/‘JX17’, was used in this study. Seed storability was investigated by using the storage property measured by the difference of seed germination rates before and after treatment of the rice seeds under 40°C and 95% relative humidity for 10 days in a phytotron. Three QTLs related to rice seed storability were detected on chromosomes 9, 11 and 12, with the LOD scores 2.76, 4.83 and 2.54, respectively, together explaining 35.4% of the genetic variation. The ‘JX17’ allele at qLS‐9 and the ‘ZYQ8’ alleles at qLS‐11 and qLS‐12 could enhance the rice seed storability. The effects of the ‘ZYQ8’ alleles of qLS‐11 and qLS‐12 were also verified using chromosome segment substitution lines. 相似文献
3.
4.
Sheng Teng Chaoguang Tian Mingsheng Chen Dali Zeng Longbiao Guo Lihuang Zhu Bin Han Qian Qian 《Euphytica》2006,152(2):141-148
Chlorate resistance is one of the reliable characters in Indica/Japonica classification. To understand the genetic basis of chlorate resistance is very important for revealing the evolutionary mechanism of Indica/Japonica differentiation. In this study, a doubled haploid (DH) population derived from anther culture of ZYQ8/JX17, a typical Indica and Japonica hybrid, was used as the genetic material to investigate chlorate sensitivity of the parents and DH lines. The quantitative trait loci (QTLs) of chlorate resistance were analyzed based on the molecular linkage map of this population. Total of 3 QTLs (qCHR-2, qCHR-8 and qCHR-10) for chlorate resistance were detected on chromosomes 2, 8 and 10, respectively. A QTL × QTL epistatic interaction was detected between qCHR-2 and qCHR-10. Genes involved in nitrogen assimilation, such as nitrate reduction, molybdenum cofactor biosynthesis and nitrate transport were strong candidates of QTLs for chlorate resistance. A putative nitrate reductase gene (8611.t00011), and two putative nitrate reductase genes (9319.t00010 and 9319.t00012) were in the genomic region of qCHR-2, and qCHR-8, respectively, and a putative nitrate transporter gene (756.t00011) was in the region of qCHR-10. The expression of 8611.t00011, 9319.t00010 and 756.t00011 were confirmed by the corresponding cDNAs, and 2 in/del and 12 SNPs in the coding regions of these three genes were found between Indica (cv. 9311) and Japonica (cv. Nipponbare) in silico. These results indicated that these three genes were candidates of the chlorate resistance QTLs. An in/del in the coding region of 8611.t00011 was used to develop a new PCR marker. A polymorphism was detected between JX17/Nipponbare and ZYQ8/9311. This polymorphism corresponds to the chlorate sensitivity of Nipponbare and 9311. This marker was located between Y8007R and RM250 on chromosome 2 in the DH population, where qCHR-2 was also located. 相似文献
5.
籼粳交加倍单倍体后代性状遗传的研究 总被引：11，自引：0，他引：11
对一个典型的籼/粳组合、窄叶青8号/京系17的F1花药进行培养,所获二倍体花粉植株除约20%自然结实率为0外,其余植株的结实率在1%至正常结实之间,65%株系在75%以上,并稳定地传至自交后代。对130多个加倍单倍体(DH)多种性状的遗传进行了研究与分析,结果表明,花药培养可以克服籼/粳后代包括育性在内的各种性状长期分离不易 相似文献
6.
Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus) 总被引：1，自引：0，他引：1
Zhen Li Shufang Mei Zhong Mei Xianglei Liu Tingdong Fu Guangsheng Zhou Jinxing Tu 《Euphytica》2014,197(3):341-353
Soil waterlogging and drought are major environmental stresses that suppress rapeseed (Brassica napus) growth and yield. To identify quantitative trait loci (QTL) associated with waterlogging tolerance and drought resistance at the rapeseed seedling stage, we generated a doubled haploid (DH) population consisting of 150 DH lines from a cross between two B. napus lines, namely, line No2127-17 × 275B F4 (waterlogging-tolerant and drought-resistant) and line Huyou15 × 5900 F4 (waterlogging-sensitive and drought-sensitive). A genetic linkage map was constructed using 183 simple sequence repeat and 157 amplified fragment length polymorphism markers for the DH population. Phenotypic data were collected under waterlogging, drought and control conditions, respectively, in two experiments. Five traits (plant height, root length, shoot dry weight, root dry weight and total dry weight) were investigated. QTL associated with the five traits, waterlogging tolerance coefficient (WTC) and drought resistance coefficient (DRC) of all the traits were identified via composite interval mapping, respectively. A total of 28 QTL were resolved for the five traits under control conditions, 26 QTL for the traits under waterlogging stresses and 31 QTL for the traits under drought conditions. Eleven QTL were detected by the WTC, and 19 QTL related to DRC were identified. The results suggest that the genetic bases of both waterlogging tolerance and drought resistance are complex. Some of the QTL for waterlogging tolerance-related traits overlapped with QTL for drought resistance-related traits, indicating that the genetic bases of waterlogging tolerance and drought resistance in the DH population were related in some degree. 相似文献
7.
甘蓝型油菜DH群体苗期抗旱性的评价 总被引：2，自引：0，他引：2
中国油菜主产区常常受到秋冬旱和春旱危害而影响产量及品质。本文通过盆栽试验,以株高(PH)、根长(RL)、地上部干重(SDW)、根干重(RDW)、根冠比(R/S)、总干重(TDW) 6个性状的抗旱系数作为抗旱性评价指标,对甘蓝型油菜DH(doubled haploid)群体的118个株系及其亲本进行苗期抗旱性评价,筛选极端抗旱DH系。结果表明,与对照相比,干旱严重抑制了甘蓝型油菜苗期的生长,6个评价指标均表现出显著差异,其中根干重的变异系数最大;在对照和干旱条件下,群体各株系各性状均表现出超亲连续分离,大部分呈正态分布,抗旱条件下的分离更为明显;相关性分析表明,地上部干重、根干重和总干重抗旱系数可作为甘蓝型油菜苗期抗旱性的主要评价指标; 相似文献
8.
Effective cumulative temperature (ECT) after heading would be a more reasonable parameter for seed sampling of pre-harvest sprouting/seed dormancy (SD) tests in segregating populations than the days after flowering. SD is an important agronomic trait associated with grain yielding, eating quality and seed quality. To identify genomic regions affecting SD at different grain-filling temperatures, and to further examine the association between SD and ECT during grain-filling, 127 double haploid (DH) lines derived from a cross between ZYQ8 (indica)/JX17 (japonica) by anther culture were analyzed. The quantitative trait loci (QTLs) and their digenic epistasis for SD were identified using a molecular linkage map of this population. A total of four putative QTLs for SD (qSD-3, qSD-5, 6 and 11) were detected on chromosomes 3, 5, 6 and 11, together explaining 41.4% of the phenotypic variation. Nine pairs of digenic epistatic loci were associated with SD on all but chromosome 9, and their contributions to phenotypic variation varied from 2.87%–8.73%. The SD QTL on chromosome 3 was identical to the QTLs found in other mapping populations with different genetic backgrounds, which could be a desirable candidate for gene cloning and marker-assisted selection in rice breeding. 相似文献
9.
Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils 总被引：1，自引：0，他引：1
Xiaoqin Zhang Guoping Zhang Longbiao Guo Huizhong Wang Dali Zeng Guojun Dong Qian Qian Dawei Xue 《Euphytica》2011,180(2):173-179
Low grain cadmium (Cd) and high grain zinc (Zn) rice cultivars have become the current rice breeding objectives. However,
the genetic control of Cd and Zn concentrations in brown rice remains poorly understood, especially when grown in Cd-contaminated
soil. In this study, quantitative trait loci (QTLs) associated with grain Cd and Zn concentrations and Cd/Zn ratio were identified
using a doubled haploid population derived from a cross between japonica JX17 and indica ZYQ8 rice cultivars. Three and two QTLs were detected for Cd and Zn concentration in brown rice, respectively. Two QTLs associated
with grain Cd/Zn ratio on chromosomes 3 and 6 were initially mapped. These QTLs accounted for 10.83–41.66% of the total variance
of the three traits measured. Only one common locus on chromosome 6 was found for Cd concentration and Cd/Zn ratio. The lack
of co-location of the QTLs for Cd and Zn concentrations in this mapping population suggests different genetic mechanisms.
In summary, our results provide insight into the genetic basis of rice grain Cd and Zn accumulation; the isolated QTLs may
be useful for marker-assisted selection and identification of genes associated with Cd and Zn accumulation in rice. 相似文献
10.
11.
用小孢子培养创建大白菜双单倍体永久作图群体 总被引：2，自引：0，他引：2
用高抗TuMv的高代自交系91-112和高感TuMV的小孢子双单倍体系T12-19杂交的F1植株进行小孢子培养，多次继代培养使小孢子再生苗安全越夏，再经移栽驯化后进行低温处理，定植到日光温室。通过加强田间管理，有效地提高了小孢子培养生产双单倍体的成功率和结实水平，得到一个具有146个白菜双单倍体系的作图群体。 相似文献
12.
Variation in root architecture is essential for the adaptation of plants to target environments. A non‐destructive gel‐based mini‐rhizotron system was used for root architecture trait phenotyping. This system has facilitated the visualization of root architectural traits in large genotype collection of rapeseed including 94 double haploid (DH) lines from “Express617” x “V8” and 439 inbred lines (ASSYST diversity set). A high‐density Express617‐V8 linkage map was used for quantitative trait loci (QTLs) identification in DH population based on standard composite interval mapping. 6K SNPs were analysed for association mapping of root traits in ASSYST diversity set. A large variation, broad segregation and medium–low heritability of root architectural traits, primary root length and growth rate, lateral root number, lateral root length and lateral root density, were observed. In the double haploid population, 11 QTL regions, and in the diversity set, 38 significant marker–trait associations were detected. Significant marker–trait associations proved that these are quantitatively inherited traits controlled by multiple genes which revealed to proceed for genetic improvement and selection of rapeseed lines with improved root system. 相似文献
13.
Haitham Sayed Hamed Kayyal Luke Ramsey Salvatore Ceccarelli Michael Baum 《Euphytica》2002,125(2):265-272
A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for
their segregation in a doubled haploid (DH) and an F2 population of barley. The DH population consisted of 71 doubled haploid lines, developed from F1 plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting
of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers
showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of
92 F2 lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population
was significantly higher (19/43loci, 44%) than in the F2 population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291
was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
A deep and thick root system has a positive effect on wheat yield, particularly in drought environments. A doubled haploid (DH) population of 150 lines derived from the cross Hanxuan 10?×?Lumai 14 was used to map QTLs for seedling root characteristics. The DH lines were cultivated in an agarose gel-chamber under well-watered (WW) and water-stressed (WS) regimes. Water stress was simulated by adding mannitol to the agarose gel. The seminal root traits, including maximum root length (MRL), seminal root number, total root length, project root area, root surface area, and seminal root angle were measured after 6?days of seedling development. Grain yields (GY) were measured in a field experiment. A total of 29 QTLs were identified for seedlings cultured under WW regimes, and 23 QTLs under WS regimes. Individual QTL accounted for phenotypic variations ranging from 4.98 to 24.31?%. The QTLs were distributed on 17 chromosomes, except 1D, 4D, 6B and 6D. Seven consistently expressed QTLs were detected for all the traits tested except MRL under both water regimes. The QTLs for root traits were unevenly distributed among chromosomes, and clustered in eight loci on seven chromosomes, showing pleiotropic effects on target traits. One region in the interval Xgwm644.2?CP6901.2 on chromosome 3B contained 9 QTLs affecting most root traits. The present data provide an insight into the genetic basis of seedling root development under different water regimes and may benefit breeding programs using marker-assisted selection (MAS) for root traits. 相似文献
15.
为探讨玉米Reid种质遗传改良效果,本研究以Reid类群重要种质J1491、J1492、J1493、J1495、J1498与PH6WC构建基础群体,通过DH技术育成DH系,经鉴定从中选取5个代表DH系6DH6、6DH7、6DH9、6DH10、6DH12作母本,以Non-Reid群5个优良自交系作父本,按NCII设计组配25个杂交组合,对DH系单株产量及其构成因素的杂种优势、配合力和相关方面进行了研究。结果表明,单株产量杂种优势以组合6DH7×J1628最高为180.22%,组合6DH12×J1673最低为54.94%;单株产量一般配合力效应值最高的有6DH7和6DH10,穗长、穗粗亦为正效应,因此认为6DH7和6DH10更具应用潜力;特殊配合力效应值最高的组合是6DH7×J1673。各产量性状中穗长、穗粗和秃尖长,狭义遗传率高,主要以基因加性效应为主。本研究为鉴定有潜在价值的DH系及杂交种提供选择,为玉米遗传改良提供参考。 相似文献
16.
Alba Rivas-Sendra Manuel Campos-Vega Antonio Calabuig-Serna Jose M. Seguí-Simarro 《Euphytica》2017,213(4):89
We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. The DH population showed segregation in vegetative traits related to leaf, flower and fruit, and in reproductive traits such as fruit and seed setting or germination rate. The DH population and subsequent generations also presented variation in the androgenic response, with null, low and high response lines. From this population, we were able to identify the first eggplant highly androgenic DH line (DH36), remarkably similar to the donor hybrid in terms of morphology and reproductive ability, but stably producing four times more calli than the hybrid. The segregating DH population is potentially useful for genetic studies and mapping of several traits, whereas the highly androgenic line DH36 may be used as a model line to facilitate the study of eggplant androgenesis and embryogenesis for both basic and applied research. 相似文献
17.
Comparative Study of CaMsrB2 Gene Containing Drought‐Tolerant Transgenic Rice (Oryza sativa L.) and Non‐Transgenic Counterpart 
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下载免费PDF全文 S. K. Dhungana B.‐R. Kim J.‐H. Son H.‐R. Kim D.‐H. Shin 《Journal of Agronomy and Crop Science》2015,201(1):10-16
Concerns regarding the safety of transgenic foods have been raised because of possibility of undesirable effects development during genetic engineering. Analysis of phenotypic traits can increase the likelihoods of identifying those unintended effects in dietary composition of the GM crops. Objective of this study was to compare the transgenic lines with their non‐transgenic counterpart. Different vegetative and reproductive traits as well as antioxidant properties were considered to evaluate the transgenic (HV8 and HV23) lines containing CaMsrB2 gene and their non‐transgenic (Ilmi) parent line. Grain size and weight, seed germination, root length, root and shoot dry weight, length and width of flag leaf, plant height, and ligule, stamen and carpel length were not significantly different. Onset and completion of heading in each line occurred almost during the same period. The antioxidant properties in terms of DPPH (1,1‐diphenyl‐2‐picrylhydrazyl) radical scavenging activity and polyphenol content were not statistically different under same treatment condition. The results suggested that the transgenic rice lines containing CaMsrB2 gene were equivalent to their non‐transgenic counterpart without any visible unintended effects. 相似文献
18.
H. R. Prabuddha K. Manjunatha R. Venuprasad M. S. Vinod J. H. Jureifa H. E. Shashidhar 《Euphytica》2008,160(3):357-368
Near-isogenic lines (NILs) constitute valuable tools in genetic investigations and plant breeding programs. Conventional methods
for developing these are time consuming and tedious. An innovative method for identifying NILs is proposed and validated.
The method involves computation of simple correlation coefficients of all possible pairs of genotypes within a mapping population
using molecular marker data, and phenotypic characterization of those pairs with very high positive correlation. The pairs
having both genomic and phenotypic similarity except for a single trait are considered as NILs. This strategy was tested with
a doubled haploid mapping population involving CT9993 and IR62266. This population was saturated with 315 markers and comprised
154 lines. The pairs showing very high correlation coefficients (0.70–0.97) and differing for less than 10% of the markers
were considered as Genotypically Closely Related Pairs (GCRPs). Graphical genotyping was employed to visualize the genome
of the closely related lines. A total of 39 such pairs were subjected to rigorous evaluation for root and shoot morphological
traits in two contrasting moisture regimes. Four GCRPs under well-watered condition and ten GCRPs under low moisture stress
condition are statistically significant for a single phenotypic trait and are considered as NILs for their respective traits
and would be the valuable materials for genetic studies. Mapped QTLs and candidate genes were employed to explain the probable
cause of phenotypic difference in NILs. 相似文献
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20.
The optimal plant growth habit and architecture of common bean (Phaseolus vulgaris L.) is dependent on environmental conditions. The objectives of this research were to determine if plant growth habit impacts
a plant’s ability to grow in low P conditions, as measured by P uptake, seed yield, and P use efficiency and to determine
if aboveground plant growth habit and root growth are associated at variable P soil levels. The study was carried out with
recombinant inbred lines developed from an Andean intra-gene pool cross between a low P tolerant parent with an indeterminate
growth habit (G19833) and a low P susceptible parent with a determinate growth habit (AND696). The population was grown for
2 years in low and sufficient P conditions in a field site in Darien, Colombia. In the first season, indeterminate lines had
15% more seed yield than the determinate lines in the low P treatment, whereas there was no difference by growth habit in
the high P treatment. In the second season, seed yield and tolerance to low P was not influenced by growth habit. Root architectural
characteristics such as root length density (RLD) and root surface area were 25% and 34% greater respectively in the indeterminate
lines under P-sufficiency, whereas under low P, root architecture traits were not significantly different by growth habit.
Root plasticity was higher in determinate lines, although RLD and root surface area did not play a significant role in tolerance
to low P. Overall, the data were consistent with shoot growth habit as playing a complex and important role in adaptation
to P-deficiency. 相似文献