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1.
《作物育种信息》2006,(3):13-14
在雨养地区和灌溉条件差的环境中，干旱是影响水稻产量及其稳定性的主要限制因子。鉴定影响产量及产量构成对干旱的反应的基因组区域将有助于我们了解耐旱性的遗传和培育更多的耐旱品种。利用CT9993—510和1-M及IR266—42和6—2杂交的154个双单倍体鉴定了产量性状及其构成成分的数量性状位点。利用线源喷灌系统进行干旱胁迫处理，该系统能够线性地降低灌溉水平，使其与生殖敏感期相一致。试验于2000年湿季在泰国乌邦水稻研究中心进行。在不同水份胁迫条件下（W0：完全灌溉；W1-W4：中等至严重缺水），鉴定到水稻产量性状及产量构成性状的数量性状位点共77个。在这77个QTL中，各性状的QTI。数量分别是：产量（GY）有7个QTL，生物产量（BY）有8QTL个，收获指数（HI）有6个QTL，灌溉开始后的开花时间有5个QTL，小穗数（TSN）有10个QTL，结实小穗数（PSS）有7个QTL，穗数有23个QTL，株高有11个QTL。单个QTL可以解释7．5—55．79／6的表型变异。在灌溉良好的条件下，我们观察到BY、HI、DFAIG、PSS、TSN、PH、GY间具有极高的遗传相关性。然而，在干旱处理中，只有BY和HI与GY具有显著的相关性。在干旱处理中，位于染色体3、4、8上的RG104至RM231，EMP2—2至RM127，G2132至RZ594两侧的QTL与GY、HI、DFAIG、BY、PSS、PN等性状相对应。这些QTL在染色体3、4、8上的聚集作用得到更高的产量。这些QTL对改良雨养水稻非常有价值，同时有利于我们了解干旱条件下生殖敏感期的遗传控制机理。紧密的遗传连锁或基因多效性可能是本试验检测到QTL重合的原因。在灌溉条件下，观察到GY、BY、HI与PSS的QTL主效应间的双基因互作。 相似文献
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在已构建的结球甘蓝AFLP、SSR和SRAP标记高密度遗传图谱的基础上,运用MapQTL 4．0软件对结球甘蓝F2群体抽薹、开花时间两性状分别进行QTL定位和分析。最终检测到2个控制甘蓝抽薹时间性状的QTL（ qbt-3-2、qbt-9-1）,以及一个同开花时间性状相关的QTL（qft-9-1）,分别位于连锁群LG3与LG9上,这3个QTL均为增效位点,共解释甘蓝抽薹、开花时间性状变异的17．5％,22．7％;同时得到与QTLs共分离的2个分子标记E46 M52-5、me26-em13-1,均可作为辅助选育甘蓝耐抽薹品种的标记使用,这将为结球甘蓝耐抽薹品种的选育提供理论依据。 相似文献
4.
以粳稻品种Asominori与籼稻品种IR24的杂交组合所衍生的染色体片段置换系（CSSLs）为材料，田间试验分别在FACE（CO2浓度约570 µmol mol-1）和对照（CO2浓度约370 µmol mol-1）下，对水稻株高性状的数量性状位点（QTL）进行了分析。结果表明，Asominori和IR24的株高、穗长、上位第一节间长和上位第二节间长在FACE和对照下的差异达显著水平；供试株系的4个株高性状对CO2浓度升高都呈正负两种响应，其变化最大的株系为AI7和AI44（株高分别增加14.2 cm和降低4.54 cm），AI9和AI12（穗长分别增加3.56 cm和降低2.39 cm），AI39和AI27（上位第一节间长分别增加15.74 cm和降低1.49 cm），AI32和AI53（上位第二节间长分别增加8.09 cm和降低3.00 cm）；FACE和对照下分别检测出14和15个QTL，分布在除第2、7、9和第10号染色体外的各染色体上，其中5个（qPH6-4、qPH8-4、qPL8-4、qPL12-4和qLFN6-4）在FACE和对照条件下同时检测到，分布在第6、8和第12染色体上，而其余的只在FACE或对照下检测到。这29个QTLs中，3个（qPH6-4QE、qPH8-4QE和qLSN5-4QE）具显著的基因型与环境互作。在不同的CO2环境下，测试性状发生不同程度的表型变异。结果推论，对CO2浓度增加敏感的QTL位点，可能受到CO2浓度增加的诱导，可见控制水稻株高性状的QTL与CO2增加的环境发生了互作效应。 相似文献
5.
水稻株高性状对大气CO2浓度升高的响应 总被引：2，自引：0，他引：2
以粳稻品种Asominori与籼稻品种IR24的杂交组合所衍生的染色体片段置换系（CSSLs）为材料,田间试验分别在FACE（CO2浓度约570 µmol mol-1）和对照（CO2浓度约370 µmol mol-1）下,对水稻株高性状的数量性状位点（QTL）进行了分析。结果表明,Asominori和IR24的株高、穗长、上位第一节间长和上位第二节间长在FACE和对照下的差异达显著水平;供试株系的4个株高性状对CO2浓度升高都呈正负两种响应,其变化最大的株系为AI7和AI44（株高分别增加14.2 cm和降低4.54 cm）,AI9和AI12（穗长分别增加3.56 cm和降低2.39 cm）,AI39和AI27（上位第一节间长分别增加15.74 cm和降低1.49 cm）,AI32和AI53（上位第二节间长分别增加8.09 cm和降低3.00 cm）;FACE和对照下分别检测出14和15个QTL,分布在除第2、7、9和第10号染色体外的各染色体上,其中5个（qPH6-4、qPH8-4、qPL8-4、qPL12-4和qLFN6-4）在FACE和对照条件下同时检测到,分布在第6、8和第12染色体上,而其余的只在FACE或对照下检测到。这29个QTLs中,3个（qPH6-4QE、qPH8-4QE和qLSN5-4QE）具显著的基因型与环境互作。在不同的CO2环境下,测试性状发生不同程度的表型变异。结果推论,对CO2浓度增加敏感的QTL位点,可能受到CO2浓度增加的诱导,可见控制水稻株高性状的QTL与CO2增加的环境发生了互作效应。 相似文献
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定位棉花种子性状的基因对揭示棉花种子性状的遗传规律,以及明确棉花种子、产量、纤维品质等性状间的遗传关系具有重要意义。以(渝棉1号×T586) F2:7重组近交系群体构建的遗传连锁图谱,在鉴定270个家系3个环境种子物理性状的基础上,利用MQM作图方法,共检测到34个种子物理性状QTL,包括9个种子重(qSW)、5个短绒重(qFW)、3个短绒率(qFP)、8个种仁重(qKW)、6个种子壳重(qHW)和3个种仁率(qKP)QTL,它们可解释4.6%~80.1%的性状表型变异。9个QTL在2个或3个环境中被检测到,其中包括第12染色体显性光子位点的短绒重与短绒率QTL,以及另外7个微效应QTL。34个QTL分布于15条染色体,其中A染色体组20个,D染色体组14个。有12个染色体区段分布有2个或2个以上的QTL,而且同一染色体区域同一亲本所具有的不同性状QTL的方向大多数与性状表型相关系数的正负一致。 相似文献
7.
用水稻测序品种培矮64s和Nipponbare为亲本构建的含137个SSRs标记的连锁遗传图谱和（培矮64s/Nipponbare）F2群体的180个单株，对水稻的单株有效穗数、穗粒数、穗实粒数、结实率、穗着粒密度、千粒重等6个产量构成性状进行了QTL定位分析。共检测到6个性状的22个QTLs，分布在第1、2、4、5、6、9、10、11、12等9条染色体的14个区域,表型贡献率5.0%-19.3%;相关性较强的性状之间具有较多共同或紧密连锁的QTLs;集中分布的QTLs之间既有同向连锁.对不同水稻群体定位的同源QTL进行了比较,对QTL在染色体上的集中分布,以及用QTL定位结果和生物信息学方法相结合预测基因的英勇等进行了探讨。 相似文献
8.
染色体片段替换系（CSSL）是基因组水平快速初步定位数量性状基因位点（QTL）的良好材料，而水稻的品质性状是多基因控制的数量性状，因此可用替换系鉴定控制水稻品质性状的QTL。郝伟等利用分子标记辅助选择技术（MAS）构建了由133个株系组成的以“特青”（籼稻品种）为轮回亲本，以海南的一种普通野生稻（外观品质较好）为供体亲本，覆盖绝大部分野生稻基因组的染色体片段替换系。利用这套替换系，初步定位了控制稻米外观和理化品质性状的15个QTL，为今后水稻品质性状QTL的克隆以及稻米品质相关性状的改良提供了依据。 相似文献
9.
水稻光合作用及相关生理性状的QTL分析 总被引：7，自引：0，他引：7
为了探讨光合作用及相关生理性状的遗传规律，利用由籼稻品种IR24 和粳稻品种Asominori杂交衍生的65个染色体片段置换系（Chromosome Segment Substitution Lines, CSSL）为材料，研究了水稻光合作用及相关生理性状的QTL。在水稻抽穗后7 d测定叶片光合速率（Pn）、蒸腾速率（Gs）、气孔导度（Tr）、细胞间隙CO2浓度（Ci）、叶绿素含量（CHL）、全氮含量（TLN）。共检测到10个QTLs，分布于第1、3、4、5、7、8和10染色体上，LOD值在2.77~8.42之间，贡献率为9.5%~46.5%。其中仅有控制气孔导度的qGs-8 与控制叶绿素含量的qCHL-8以及第10染色体上控制气孔导度的qGs-10与控制细胞间隙CO2浓度的qCi-10位置相同，分别位于第8染色体上标记R727和第10染色体上标记C1166附近。其他QTL在染色体上的位置不同，暗示了水稻光合功能遗传规律的复杂性。 相似文献
10.
利用RIL群体对水稻再生力及相关农艺性状的QTL分析 总被引：1，自引：0，他引：1
以粳糯稻品种糯89-1与籼型重穗型杂交稻骨干恢复系蜀恢527杂交构建的籼粳交F7代RIL群体的169个家系为作图群体,构建了一张含105个微卫星(SSR)标记的分子连锁图谱。定位了水稻正季7个农艺性状的QTL 15个,分布在第1、第2、第3、第5、第6、第7、第10染色体上,LOD值介于2.10~7.51,贡献率3.77%~25.37%,其中贡献率10.0%以上的QTL 7个,单个性状的QTL 1~4个;定位了水稻再生季7个农艺性状的QTL 19个,分布在第1、第2、第3、第4、第5、第6、第7、第10染色体上,LOD值介于2.17~18.34,贡献率3.23%~37.66%,其中贡献率10.0%以上的QTL 7个,单个性状的QTL1~5个;定位了影响水稻再生力(最终再生率)的QTL 2个(qRa4,qRa5),分别在第4和第5染色体上,贡献率分别为8.17%和7.09%,加性效应分别为0.32和-0.39,贡献率和加性效应均较小,属微效基因。共检测到两季农艺性状QTL 36个,同一性状被重复检测的QTL 8个。水稻再生力与正季稻有效穗呈极显著负相关;水稻再生力与再生稻有效穗呈极显著正相关,与每穗总粒数和着粒密度呈显著负相关。QTL定位结果揭示了有效穗是影响再生力的主要因素。 相似文献
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QTL analysis of seed storability in rice 总被引：7，自引：0，他引：7
A double haploid population, which consists of 127 lines derived from anther culture of a typical indica and japonica hybrid ‘ZYQ8’/‘JX17’, was used in this study. Seed storability was investigated by using the storage property measured by the difference of seed germination rates before and after treatment of the rice seeds under 40°C and 95% relative humidity for 10 days in a phytotron. Three QTLs related to rice seed storability were detected on chromosomes 9, 11 and 12, with the LOD scores 2.76, 4.83 and 2.54, respectively, together explaining 35.4% of the genetic variation. The ‘JX17’ allele at qLS‐9 and the ‘ZYQ8’ alleles at qLS‐11 and qLS‐12 could enhance the rice seed storability. The effects of the ‘ZYQ8’ alleles of qLS‐11 and qLS‐12 were also verified using chromosome segment substitution lines. 相似文献
12.
Cadmium (Cd) is a non-essential element and toxic to plants. To investigate the genetics of Cd tolerance and accumulation
in rice, quantitative trait loci (QTL) associated with Cd tolerance and accumulation at the seedling stage were mapped using
a doubled haploid (DH) population derived from a cross between a japonica JX17 and an indica ZYQ8. A total of 22 QTLs were found to be associated with shoot height (SH), root length (RL), shoot dry weight (SDW), root
dry weight (RDW), total dry weight (TDW) and chlorophyll content (CC), and 10 and 12 QTLs were identified under the control
and Cd stress conditions, respectively. For Cd tolerant coefficient (CTC), 6 QTLs were detected on chromosomes 1, 3, 5, 8
and 10. Under Cd stress, 3 QTLs controlling root and shoot Cd concentrations were mapped on chromosome 6 and 7. One QTL for
shoot/root rate of Cd concentration was identified on chromosome 3. The results indicated that Cd tolerance and accumulation
were quantitatively inherited, and the detected QTLs may be useful for marker-assistant selection (MAS) and identification
of the genes controlling Cd tolerance and accumulation in rice. 相似文献
13.
Effective cumulative temperature (ECT) after heading would be a more reasonable parameter for seed sampling of pre-harvest sprouting/seed dormancy (SD) tests in segregating populations than the days after flowering. SD is an important agronomic trait associated with grain yielding, eating quality and seed quality. To identify genomic regions affecting SD at different grain-filling temperatures, and to further examine the association between SD and ECT during grain-filling, 127 double haploid (DH) lines derived from a cross between ZYQ8 (indica)/JX17 (japonica) by anther culture were analyzed. The quantitative trait loci (QTLs) and their digenic epistasis for SD were identified using a molecular linkage map of this population. A total of four putative QTLs for SD (qSD-3, qSD-5, 6 and 11) were detected on chromosomes 3, 5, 6 and 11, together explaining 41.4% of the phenotypic variation. Nine pairs of digenic epistatic loci were associated with SD on all but chromosome 9, and their contributions to phenotypic variation varied from 2.87%–8.73%. The SD QTL on chromosome 3 was identical to the QTLs found in other mapping populations with different genetic backgrounds, which could be a desirable candidate for gene cloning and marker-assisted selection in rice breeding. 相似文献
14.
多环境下稻米粒重的QTL定位 总被引：2，自引：0，他引：2
以粳稻Asominori为遗传背景的染色体片段置换系（CSSLs）群体为材料,利用基于性状-标记多元回归分析方法对稻谷粒重和精米粒重进行多环境的QTL定位。结果在5个环境共检测到6个粒重相关QTL,分布于第1、6、7和8染色体上,对表型变异的贡献率介于13%~35%;其中控制精米粒重的qMRW-1a和稻谷粒重的qPRW-1在不同环境中均能稳定表达,且均位于第1染色体RFLP标记XNpb113附近,该基因座还同时控制着粒宽。qMRW-1a和qPRW-1共同对应的置换系AIS8和AIS11与Asominori 的粒重差异在不同环境中均显著(P < 0.05),表明该QTL的等位基因在不同环境中效应显著。比较发现该QTL在不同遗传群体中均能被重复检测到,且与蔗糖磷酸合酶基因（SPS）位置一致,推测该QTL与淀粉合成代谢有关。qMRW-1a 和qPRW-1在不同环境条件和遗传背景中表达,因此可用于进一步的精细定位研究。 相似文献
15.
Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils 总被引：1，自引：0，他引：1
Xiaoqin Zhang Guoping Zhang Longbiao Guo Huizhong Wang Dali Zeng Guojun Dong Qian Qian Dawei Xue 《Euphytica》2011,180(2):173-179
Low grain cadmium (Cd) and high grain zinc (Zn) rice cultivars have become the current rice breeding objectives. However,
the genetic control of Cd and Zn concentrations in brown rice remains poorly understood, especially when grown in Cd-contaminated
soil. In this study, quantitative trait loci (QTLs) associated with grain Cd and Zn concentrations and Cd/Zn ratio were identified
using a doubled haploid population derived from a cross between japonica JX17 and indica ZYQ8 rice cultivars. Three and two QTLs were detected for Cd and Zn concentration in brown rice, respectively. Two QTLs associated
with grain Cd/Zn ratio on chromosomes 3 and 6 were initially mapped. These QTLs accounted for 10.83–41.66% of the total variance
of the three traits measured. Only one common locus on chromosome 6 was found for Cd concentration and Cd/Zn ratio. The lack
of co-location of the QTLs for Cd and Zn concentrations in this mapping population suggests different genetic mechanisms.
In summary, our results provide insight into the genetic basis of rice grain Cd and Zn accumulation; the isolated QTLs may
be useful for marker-assisted selection and identification of genes associated with Cd and Zn accumulation in rice. 相似文献
16.
Sheng Teng Chaoguang Tian Mingsheng Chen Dali Zeng Longbiao Guo Lihuang Zhu Bin Han Qian Qian 《Euphytica》2006,152(2):141-148
Chlorate resistance is one of the reliable characters in Indica/Japonica classification. To understand the genetic basis of chlorate resistance is very important for revealing the evolutionary mechanism of Indica/Japonica differentiation. In this study, a doubled haploid (DH) population derived from anther culture of ZYQ8/JX17, a typical Indica and Japonica hybrid, was used as the genetic material to investigate chlorate sensitivity of the parents and DH lines. The quantitative trait loci (QTLs) of chlorate resistance were analyzed based on the molecular linkage map of this population. Total of 3 QTLs (qCHR-2, qCHR-8 and qCHR-10) for chlorate resistance were detected on chromosomes 2, 8 and 10, respectively. A QTL × QTL epistatic interaction was detected between qCHR-2 and qCHR-10. Genes involved in nitrogen assimilation, such as nitrate reduction, molybdenum cofactor biosynthesis and nitrate transport were strong candidates of QTLs for chlorate resistance. A putative nitrate reductase gene (8611.t00011), and two putative nitrate reductase genes (9319.t00010 and 9319.t00012) were in the genomic region of qCHR-2, and qCHR-8, respectively, and a putative nitrate transporter gene (756.t00011) was in the region of qCHR-10. The expression of 8611.t00011, 9319.t00010 and 756.t00011 were confirmed by the corresponding cDNAs, and 2 in/del and 12 SNPs in the coding regions of these three genes were found between Indica (cv. 9311) and Japonica (cv. Nipponbare) in silico. These results indicated that these three genes were candidates of the chlorate resistance QTLs. An in/del in the coding region of 8611.t00011 was used to develop a new PCR marker. A polymorphism was detected between JX17/Nipponbare and ZYQ8/9311. This polymorphism corresponds to the chlorate sensitivity of Nipponbare and 9311. This marker was located between Y8007R and RM250 on chromosome 2 in the DH population, where qCHR-2 was also located. 相似文献
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Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton 总被引：21，自引：1，他引：21
Xinlian Shen Wangzhen Guo Qiongxian Lu Xiefei Zhu Youlu Yuan Tianzhen Zhang 《Euphytica》2007,155(3):371-380
The improvement of cotton fiber quality has become more important because of changes in spinning technology. Stable quantitative
trait loci (QTLs) for fiber quality will enable molecular marker-assisted selection to improve fiber quality of future cotton
cultivars. A simple sequence repeat (SSR) genetic linkage map consisting of 156 loci covering 1,024.4 cM was constructed using
a series of recombinant inbred lines (RIL) developed from an F2 population of an Upland cotton (Gossypium hirsutum L.) cross 7235 × TM-1. Phenotypic data were collected at Nanjing and Guanyun County in 2002 and 2003 for 5 fiber quality
and 6 yield traits. We found 25 major QTLs (LOD ≥ 3.0) and 28 putative QTLs (2.0 < LOD < 3.0) for fiber quality and yield
components in two or four environments independently. Among the 25 QTLs with LOD ≥ 3, we found 4 QTLs with large effects on
fiber quality and 7 QTLs with large effects on yield components. The most important chromosome D8 in the present study was
densely populated with markers and QTLs, in which 36 SSR loci within a chromosomal region of 72.7 cM and 9 QTLs for 8 traits
were detected. 相似文献
19.
Boyin Qiu Weihui Zhou Dawei Xue Fanrong Zeng Shafaqat Ali Guoping Zhang 《Euphytica》2010,174(2):199-207
Identification of Cr-tolerant lines in a rice DH population was conducted based on the modified weighted function analysis.
The significant difference was found between the two parents and among DH population lines, with lines 117, 101 and parent
ZYQ8 showing the high Cr tolerance, lines 41 and 49 showing Cr sensitivity. A dramatic difference also existed in Cr accumulation
of plant tissues, with lines 19, 18 and 1, 5 having the minimum and maximum shoot Cr accumulation, respectively, and line
19, parent JX17 and lines 1, 56, ZYQ8 having the minimum and maximum root Cr accumulation, respectively. There was a significant
difference in shoot/root ratio of Cr accumulation among the population, with line 18 and parent ZYQ8 ranking the tops and
lines 109, 71, 19, parent JX17 ranking the bottoms. Zn uptake and accumulation was reduced when the plants were exposed to
Cr stress. In addition, three QTLs were detected, which are, respectively, associated with Cr accumulation in shoot and root,
and ratio of shoot to root. 相似文献