共查询到18条相似文献，搜索用时 343 毫秒
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Suo Jia-jia Cao Rong-feng Cui Xiao-ni Jiang Zhong-ling Cong Xia Cui Kai Tian Wen-ru 《东北农业大学学报(英文版)》2014,21(2):38-45
The objective of the paper was to detect HSP72 expression and HSP72 gene sequence in heat shocked mouse preimplantation embryos and the effects of different thermo conditions on hatching rates of embryos. The mouse blastocysts cultured in vitro were heat treated at 40℃ and 38℃ for 1 h, 2 h and 3 h and then recovered at 370C for 3 h, 2 h and 1 h, respectively, to detect their HSP72 gene expression by using RT-PCR after the total R.NA extraction. The hatching rate of the blastocysts for different treated groups was recorded and the expression of liSP72 in the blastocysts was determined by Western blot. The results showed that all the groups of blastocysts, including the control, had the expression of HSP72 gene. The expression of HSP72 protein had the highest level in the embryos stressed at 38℃ for 2 h, and it was significantly higher than that in the control group. The expression of HSP72 in the groups of blastocysts treated at 40℃ was not significantly different from that in the control group. The embryos with induction of mild heat shock at 38℃ for 2 h, then subjected to heat shock at 40℃ for 2 h, had a significant higher （P〈0.05） hatching rate of 54.74% compared to 47.85% in the embryos treated directly at 40℃ for 2 h. The above results indicated that the mouse blastocysts were sensitive to heat shock and a mild heat shock induced HSP72 gene expression. Induction of HSP72 expression with mild heat shock helped embryos to tolerate more severe heat shocks. 相似文献
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WANG Bao-wei WU Xiao-ping ZHANG Xu-hui JIA Xiao-hui ZHANG Ming-ai LONG Fang-yu YANG Zhi-gang WANG Lei 《中国农业科学(英文版)》2008,7(2):239-247
The experiment was conducted to study the specific expression of HSP70 caused by heat shock, HSP70 purification and the characteristics of coalescence with antigenic peptide in the formation of the complex. Sixty healthy 6-week-old male Wulong geese were selected and randomly divided into three groups. The control group was slaughtered without heat treatment. Treatment group 1 was shocked with an acute heat treatment at （42 ± 1）℃ for 5 h before they were immediately slaughtered. Treatment group 2 was kept for 12 h after the heat treatment under normal conditions in order to recover and was then slaughtered. Cardiac tissue was taken in order to make paraffin sections for the immunohistochemistry experiment and the liver tissue was used to purify HSP70. The geese heart HSP70 expression differences in the three groups were determined and at the same time the experiments of HSP70 purification and appraisal in the liver tissue were carried on. HSP70 purification and synthesis of HBV PreS1 multi-peptides unified the complex, which was determined by bi-specific antibody enzyme-linked immune sandwich assay. The results indicated that widespread HSP70 positive pellets in the cardiac muscle were found under hot shock conditions. HSP70 expression in the treatment group 1 was centered in the karyotheca and its periphery, but in treatment group 2, it was centered in the surrounding cell membrane. The HSP70 purification could be obtained through two sets of purification plans; both the synthesis peptide and the HSP70 purification form the complex under certain conditions. The double antibody sandwich ELISA technique was applied to detect if the complex had been formed. Positive results showed that the complex was formed. The specific expression of HSP70 under heat shock shifted with time, suggesting that HSP70 possibly had some function in cell protection. High-purity HSP70 protein can be obtained under low-pressure chromatography conditions, and in comparison with each other, it was better in the flow of the molecular 相似文献
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CHENG Guo LI Xue-jun QIAO Zhi-gang SHI Ling PENG Xin-liangCollege of Life Science Henan Normal University Xinxiang 《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [Mthod] The Silurus astus sperms were irradiated by ultraviolet,then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.[Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization,and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5%). [Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files for further study of gynogenesis development mechanism. 相似文献
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The presence of abundant and diverse communities of macro-arthropods is considered an indicator of sustainability in agroecosystems. This study was designed to investigate the effects of different fertilizer treatments on abundance and diversity of insects of arable loess soil on the Loess Plateau of China. These regimes included a control with no fertilizer addition or manure, treatments with application of mineral fertilizers (N, NK, NP, PK, NPK), treatments with NPK in combination with organic materials such as wheat straw or maize stalk, treatments with two rates of organic manure application; and different crop rotations (Rot.1: winter wheat summer maize; Rot.2: winter wheat summer maize soybean intercropping; and Rot.3: winter wheat or rapeseed summer maize soybean intercropping). Soil macro-arthropods were collected from the plough layer (0-20 cm) and sorted by hand after each harvest in June and October 2001 and 2002. A total of 3 132 individuals were collected, from 7 orders and 55 families, dominated by Formicidae (61.72%) and Staphylinidae (14.24%). The results showed that individuals and groups were significantly influenced by sampling dates, while groups were significantly influenced by the fertilization treatments. Soil insect biodiversity, as determined by the Shannon index, was significantly influenced by fertilization and sampling dates. The abundance of soil insects was positively and significantly correlated with soil moisture content in October 2002. Nitrogen, phosphorus and potassium fertilizers and incorporation of organic materials were favorable factors for abundance and diversity in arable loess soil. 相似文献
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《农业科学学报》2017,(11)
The effect of long-term fertilization on soil denitrifying communities was analysed by measuring the abundance and diversity of the nitrous oxide(N_2O)reductase gene,nos Z.Soil samples were collected from plots of a long-term fertilization experiment established in 1982 in Suining City,China.The fertilizer treatments were no fertilizer(CK),three chemical fertilizer(CF)treatments(N,NP,NPK),manure(M)alone,and manure with chemical fertilizers(NM,NPM,NPKM).The abundance and diversity of the denitrifying bacteria were assessed by real-time quantitative PCR,terminal restriction fragment length polymorphism(T-RFLP),and cloning and sequencing of nos Z genes.The diversity and abundance of nos Z-denitrifiers was higher in soil amended with manure and chemical fertilizers(CFM)than in soil amended with CF alone,and the highest in topsoil(0–20 cm).The nos Z-denitrifier community composition was more complex in CFM soil than in CF soil.Specific species were detected only in the CFM soil.The abundance of nos Z-denitrifier in the NPKM treatment was approximately two times higher than that in the CK,N,and NPK treatments.Most of the cloned nos Z sequences were closely related to nos Z sequences from Bradyrhizobiaceae and Rhodospirillaceae in Alphaproteobacteria.Of the measured abiotic factors,soil organic matter correlated significantly with the abundance(P0.01);available phosphorus correlated significantly with the topsoil community composition(P0.01),whereas soil organic matter correlated significantly with the subsoil(20–90 cm)community composition(P0.01).This study demonstrated that long-term CFM fertilization affected both the abundance and composition of the nos Z-denitrifier community. 相似文献
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《东北农业大学学报(英文版)》2016,(4):61-66
The aim of the study was to assess effect of the atipamezole on talezol/xylazine induced expression of c-fos in rat brain. Rats were injected with the mixture of 13.81 mg · kg~(-1) telazol and 5.21 mg · kg~(-1) xylazine, following 10 min later0.522 mg · kg~(-1) atipamezole injected, and then the cerebral cortex and thalamencephal were removed at 1 h after injected. Level of Fos protein was measured in the brain tissue by western-blot. The results revealed that telazol/xylazine induction Fos protein expression in the thalamencephal and cerebral cortex during the period of anesthesia, atipamezole attenuated telazol/xylazine induction Fos protein expression in the thalamencephal and cerebral cortex. The results indicated that atipamezole could inhibite telazol/xylazine-induced c-fos expression in the rat brain, and played a protective role of neuronal injury. 相似文献
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《农业科学学报》2016,(8)
The small heat shock protein(sHSP) chaperones are required for protecting cellular proteins from damage,as well as refolding denatured proteins.This study was carried out to investigate the temporal-spatial expression patterns of two sHSP genes in rice.These two genes,named as Os16.9A and Os16.9B,are reverse duplicated genes that adjacently located on chromosome 1 and probably share the same or overlapping DNA region as a promoter.The interval sequence between the start codons of the two genes which are transcribed in opposite directions is only about 2.6 kb.Semi-quantitative RT-PCR was carried out to detect the expression of the two genes under normal growth conditions and different stress conditions.The expression patterns of the two genes were in detail investigated by using β-glucuronidase(GUS) reporter gene fusion system.Results showed that heat shock stress can induce high level expression of the two genes.Under normal growth conditions,Os16.9A and Os16.9B expressed in vegetative organs and young panicles.GUS staining combined with cytological observations showed that the two genes expressed mainly in the vascular tissues of roots,stems and young panicles,implicating that Os16.9A and Os16.9B play important roles not only for heat shock response,but also for normal development in rice. 相似文献
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冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育 总被引：1，自引：0，他引：1
[目的]通过冷休克法诱导黄河鲇二倍体雌核发育。[方法]在水温23.5±0.5℃的条件下,用紫外线照射黄河鲇(Silurus asotus)精子,然后进行进行人工受精,设置不同的冷休克起始时间和持续处理时间,观察黄河鲇二倍体雌核发育。[结果]在紫外线照射15min、冷休克起始时间为受精后5 min、持续处理40 min的条件下得到的二倍体黄河鲇成活率最高,达8.5%。[结论]该研究为培育优良黄河鲇新品种奠定了基础,为深入研究雌核发育机理积累了原始资料。 相似文献
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为探索无需卵子失活仅用冷休克方法诱导雄核发育单倍体,以红鳍东方鲀Takifugu rubripes为研究对象,试验选用L_9(3~4)设计,进行3因素3水平的正交试验,处理温度设为0、2、4℃,处理持续时间设为30、45、60 min,处理起始时间设为2、5、8 min,共9个试验组,并对其后代采用单个胚胎染色体计数法进行染色体数目统计及微卫星分析。结果表明:冷休克诱导得到的单倍体率最高为第7试验组和第9试验组,分别为受精后8 min在4℃下处理30 min和受精后5 min在4℃下处理60 min,单倍体率分别为86.7%和80.0%;根据正交试验直观分析结果,得出冷休克诱导红鳍东方鲀雄核发育单倍体的3因素最优组合为处理温度4℃、处理持续时间60 min、处理起始时间8 min;影响冷休克诱导单倍体的3因素主次顺序为处理温度处理起始时间处理持续时间;微卫星分析结果表明,单倍体携带的遗传基因全部为父本的遗传基因。研究表明,仅用冷休克方法可以成功诱导红鳍东方鲀雄核发育单倍体,雄核发育单倍体率高达86.7%,为进一步研究诱导红鳍东方鲀雄核发育二倍体奠定了基础。 相似文献
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Induction of Gynogenesis of Silurus astus Using Cold Shock 总被引：2，自引：0，他引：2
[Objective] The cold shock method was used to induce diploid gynngenesis of Silurus astus. [Mthod] The Silurus astus sperms were irradia-ted by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus as-tus. [Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization, and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5% ). [Conclusion] The experiment laid foundation for culturing new excellent Silu- rus astus variety and accumulated original files for further study of gynogenesis development mechanism. 相似文献
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黄粉虫幼虫抗菌肽的诱导特性及电泳组分研究初报 总被引：4，自引：0，他引：4
对黄粉虫幼虫分别进行空白对照、菌刺、菌饲、热激和超声波四种处理,并于处理后1d、2d、3d、4d、5d分别提取黄粉虫幼虫体内的抗菌肽,做抗大肠杆菌（革兰氏阴性菌）和抗金黄色葡萄球菌（革兰氏阳性菌）试验及交叉抑菌试验,测定其抑菌圈大小,确定其抗菌活性和诱导特异性,同时还进行抗菌肽热稳定性及电泳试验。结果表明：水浴后提取的抗菌液抑菌效果明显,四种处理的黄粉虫幼虫均能产生抗大肠杆菌／金黄色葡萄球菌物质。其中抗金黄色葡萄球菌效果要略好于抗大肠杆菌效果,其抗菌效果显著性依次为：菌饲、对照、热激和超声波、菌刺,而抗大肠杆菌效果显著性则依次为：菌饲、菌刺、热激和超声波、对照。交叉抑菌试验表明黄粉虫幼虫体内抗菌肽的产生不具有诱导特异性。电泳图谱显示黄粉虫抗菌肽含有5种肽组分。 相似文献
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【目的】筛选出有效的凡纳滨对虾人工授精方法,为后续的雌核发育诱导研究奠定基础。【方法】以受精率和幼体孵化率为考核指标,探讨外体精卵结合授精法、精荚移植法、精液移植法等不同人工授精技术对凡纳滨对虾繁育效果的影响。【结果】体外精卵结合授精法在各处理组均无幼体成功孵出,但在精子密度为2×107个/L和卵子密度为300枚/L,且在卵排出1 min内完成授精操作时,可获得相对较高的受精率(6.67%)。在精液移植法中,发现精荚在8℃恒温条件下保存3h内进行授精操作,仍可获得一定的受精率和幼体孵化率,超过3h后则无法实现受精;精荚保存温度以8~16℃为宜,保存温度升至25℃时则无法实现受精。3种人工授精技术中,以精液移植法的受精率和幼体孵化率最高,分别为52.33%和12.35%,其次是精荚移植法,体外精卵结合授精法的效果最差,无幼体孵出。【结论】精液移植法是凡纳滨对虾最有效的人工授精方法,但精荚保存时间不宜超过3 h,且应在雌虾排卵后1~2 min完成挤精荚和网搓释放精子溶液,同时可添加预先收集浓缩制备好的卵水溶液,以提高凡纳滨对虾的人工授精效率。 相似文献
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采用同工酶电泳的方法,研究了紫外(ultraviolet,UV)胁迫条件下对棉铃虫体内酯酶、过氧化物酶(peroxidase,POX)及过氧化氢酶(catalase,CAT)等同工酶的影响。结果表明:与对照相比,紫外线照射处理组酯酶同工酶谱带发生了质和量的变化,照射时间为30min和60min时,谱带E4、E9、E10增强,谱带E2、E8减弱,谱带E1、E5、E7、E11消失,新增了谱带E3、E6;照射时间延长至90min时,谱带E4、E9增强,谱带E2、E8减弱,谱带E1、E5、E7消失,新增了谱带E3、E6。紫外线照射处理下,棉铃虫POX同工酶谱带P5有所增强,CAT同工酶谱带的变化因照射时间不同而有所差异。与对照相比,紫外线照射处理组的C1谱带增强,但C2谱带在30min时减弱,60min时恢复到对照水平,90min时与对照相比又有所减弱。 相似文献