中国牡丹染色体研究进展   总被引：7，自引：0，他引：7  

侯小改  段春燕  刘素云  吕静霞  张亚冰  李嘉珏 《中国农学通报》2006,22(2):307-307

染色体是基因的载体，是生物进化发育、遗传变异的物质基础。开展对牡丹染色体的研究，不仅可以从细胞水平上了解遗传变异的规律，而且可以研究不同物种遗传的变异。因此，综述了中国牡丹的染色体研究现状及进展，着重介绍了中国牡丹野生种及栽培种的染色体数目、核型、Giemsa C带、银染带等研究成果。结果表明：牡丹种及品种的核型相当稳定，很难作为鉴别种类的依据。但核型分析结果可为确定野生种分类地位、演化关系及为遗传育种工作提供细胞学依据。染色体C带及银染带具有种的特异性，可作为鉴别牡丹种及品种的重要依据。  相似文献   

Sequential combinations of C-banding and in situ hybridization and their use in the detection of interspecific introgressions into wheat     

Angeles Cuadrado  Pilar Rubio  Esther Ferrer  Nicolás Jouve 《Euphytica》1996,89(1):107-112

Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.  相似文献   

种子贮藏蛋白凝胶电泳在小麦外源染色体鉴定中的应用   总被引：3，自引：0，他引：3  

王小利  张改生  陈新宏 《西北农林科技大学学报(自然科学版)》2004,32(1):19-22

利用小麦种子贮藏蛋白凝胶电泳技术,对簇毛麦(Haynaldiavillosa)、滨麦(Leymusmollis)、华山新麦草(Psathyrostachyshuashanica)、大麦(Hordeumdistichom)以及黑麦(Secalecereale)的高分子量谷蛋白亚基和醇溶蛋白进行了分析。结果表明,簇毛麦、滨麦、华山新麦草、大麦的高分子量谷蛋白亚基和醇溶蛋白与普通小麦相比均有各自的特征带;利用簇毛麦和大麦的高分子量谷蛋白亚基特征带鉴定出了普通小麦中与小麦第一同源群染色体有部分同源的V1和H5附加系;利用黑麦1RS醇溶蛋白标记位点Gld1B3鉴定了1BL/1RS易位系,同时利用染色体C-分带对1BL/1RS易位系77(2)进行了验证。并对小麦种子贮藏蛋白凝胶电泳技术在小麦品质育种和细胞质雄性不育选育中的应用进行了讨论。  相似文献   

犬草(Elymus caninus)的染色体C带     

杨瑞武  周永红  郑有良 《四川农业大学学报》2003,21(3):193-195

利用改良的GiemsaC带技术,分析了犬草(Elymuscaninus)的染色体C带带型。其染色体核型是:2n=4x=28=2M+24m+2sm。不同染色体之间的C带带型在带的数量、大小、强弱及位置等方面存在明显差异,因此认为C带带型可作为犬草染色体的细胞学标记。  相似文献   

金曼龙鱼的核型和C-带     

陈友铃  郑翠芳  汪彦愔  吴文珊 《福建农业学报》2007,22(1):46-49

以肾细胞作材料,采用秋水仙素-低渗-空气干燥法、Sumner(1972)的方法(C-带),制作染色体标本,研究了金曼龙(Trichogaster microlepis)的核型及C-带。结果显示:金曼龙的核型公式为2n=46 t,NF=46。多数染色体的着丝点和端部均显示出一个深浅不同的C-带,没有发现异型性染色体.并通过与同科种类进行对比分析,讨论它们的亲缘关系和系统演化。  相似文献   

黑熊染色体C-带的研究   总被引：1，自引：0，他引：1  

张颖  金日明  方南洙  张星吉  李钟淑 《延边大学农学学报》2006,28(3):187-190,204

采用BSG方法研究了22头黑熊的C-带．结果表明：阳性带分布于所有端着丝粒染色体的着丝粒部位，其余部位均浅染；中间着丝粒染色体和X染色体呈弱阳性；Y染色体淡染．而且C-带出现多态性，不同细胞染色体间C-带的大小有明显差异．  相似文献   

Application of C-banding and fluorescence in situ hybridization for the identification of the trisomics of Hordeum chilense   总被引：1，自引：0，他引：1  

A. Cabrera  M.C. Ramirez  A. Martin 《Euphytica》1999,109(2):123-129

Hordeum chilense is a wild barley species that has a high degree of genetic variability and significant potential for use in plant breeding. To establish a series of trisomics in H. chilense (2n = 14), plants with 2n + 1 chromosome numbers were isolated from the progenies of selfed triploid plants. Based on both fluorescent in situ hybridization with pAs1 and pTa71 repetitive DNA probes and C-banding patterns, seven different trisomics were tentatively identified. Primary trisomic plants were for chromosomes 1H^ch, 4H^ch, 5H^ch, 6H^ch and 7H^ch. A secondary trisomic carrying a 5H^chS-5H^chS isochromosome as the extra chromosome and a trisomic for chromosome 3H^ch heterozygous for the 3H^chS-4H^chL and 4H^chS–3H^chL interchange were identified. The trisomic for chromosome 1H^ch cannot be phenotypically distinguished from the diploid. The rest of the trisomic types were distinguishable from the diploid by their morphological characteristics (relatively poor vigour, decreased size and shorter spikes) but they were morphologically indistinguishable from each other. The frequencies of trisomics among the progenies derived from self-fertilization of these aneuploids ranged from 10.7% to 37.5%, with an average frequency of 26.1%. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

提莫菲维小麦与光稃野燕麦易位系TGF₃14的顺序C-分带-基因组原位杂交鉴定          下载免费PDF全文

郑会敏  王琳  安洪周  李鹏飞  张素勤  耿广东  张庆勤 《西北农业学报》2013,22(12):21-24

采用顺序C-带-基因组原位杂交（GISH）技术对提莫菲维小麦与光稃野燕麦远缘杂交后代中的外源遗传物质进行鉴定。GISH分析结果表明，在根尖细胞有丝分裂中期染色体具有2对清楚的杂交信号，说明该后代中可能含有稳定的外源遗传物质，且以易位的染色体片段形式存在。对该后代进行顺序C-带-GISH分析将杂交信号分别定位于提莫菲维小麦的7A和6G染色体短臂上，提莫菲维小麦的7A和6G染色体中含有光稃野燕麦的遗传物质。  相似文献