徐淮白山羊染色体核型研究1     

房兴堂  陈宏  朱必才  范智伟  孙晶晶 《中国草食动物》2005,25(3):16-18

采用外周血淋巴细胞培养及染色体分带技术分析了徐淮白山羊的染色体核型、C-带。结果表明，徐淮白山羊二倍体染色体数为2N=60，常染色体和X染色体均为端部着丝粒染色体．X染色体的大小介于1号和2号染色体之间，Y染色体最小．为中部着丝粒染色体，公羊核型为60．XY．母羊为60．XX。大部分常染色体和性染色体着丝粒部位显示阳性C带。  相似文献   

普通小麦-簇毛麦易位系T6BS·6BL-2VS的选育(英文)   总被引：2，自引：0，他引：2  

陈全战  张边江  周峰  吴梅  李华春 《农业科学与技术》2008,9(4):47-50

[Objective] The aim of experiment was to provide a new germplasm for wheat breeding by further using desirable genes in 2V chromosome of Haynaldia villosa.[Method] Through hybridization between common wheat(Triticum aestivum)-Haynaldia villosa disomic substitution line and common wheat Nonglin26-3C chromosome of Aegilops triuncialis disomic addition line,the analysis methods such as chromosome C-banding,genomic in situ hybridization and molecular marker technique were comprehensively applied and combined characters investigation.[Result] The wheat-Haynaldia villosa translocation line(T6BS·6BL-2VS)was selected from hybrid progenies to conduct characters investigation,which found some bristles on glume ridge of T6BS·6BL-2VS.[Conclusion] The translocation line induced by gametocidal chromosome was a small segment translocation line and the gene of bristle on glume ridge of Haynaldia villosa was located between the middle and the terminal of 2VS.  相似文献   

Structural Changes of 2V Chromosome of Haynaldia villosa Induced by Gametocidal Chromosome 3C of Aegilops triuncialis     

Quan-zhan CHEN Ph D  Ai-zhong CAO  Zeng-jun QI  Wei ZHANG  Pei-du CHEN   《中国农业科学(英文版)》2008,7(7):804-811

Haynaldia villosa （2n=2X= 14, VV）, a relative of wheat, plays important roles in wheat improvement mainly owing to its disease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into wheat by Cytogenetic Institute, Nanjing Agricultural University, China, and is widely used in the current wheat breeding programs. In this research, our objective is to further transfer and utilize the beneficial genes such as eye-spot resistance, yellow rust resistance, and gene of the tufted bristles on the glume ridge （a remarkable morphology） mapped on 2V of Haynaldia villosa. A disomic addition line with gametocidal chromosome 3C ofAegilops triuncialis added in Norin-26 was crossed to the wheat-H, villosa disomic substitution 2V（2D） and the hybrid F1 was then self-crossed. Chromosome C-banding, genomic in situ hybridization （GISH）, and meiotic analysis in combination with molecular markers were applied to detect the chromosome variations derived from hybrids Fz and F3. To date, four translocations including one small segmental translocation T6BS·6BL-2VS, two whole arm translocations （preliminarily designed as T3DS·2VL and T2VS.7DL） and one intercalary translocation T2VS·2VL-W-2VL, one deletion Del. 2VS·2VL-, one monotelosomic Mt2VS, and one isochromosome 2VS·2VS line have been developed and characterized. One wheat SSR marker Xwmc25.120 tagging 2VS and one wheat STS marker NAU/STSBCD135-1 （2BL） tagging 2VL were successfully used to confirm the alien chromosome segments involved in the seven lines. The tufted bristles on the glume ridge appeared in lines T2VS-7DL, Mt2VS, 2VS-2VS as well as the parent DS2V（2D）, whereas in T3DS·2VL, this trait did not appear. The gene controlling the tufted bristles was located on 2VS. Gametocidal chromosome 3C ofAegilops triuncialis could successfully induce chromosome 2V structural changes.  相似文献   

Giemsa C-banding in Avena insularis Ladizinsky     

E.N. Jellen  G. Ladizinsky 《Genetic Resources and Crop Evolution》2000,47(3):227-230

The Sicilian wild tetraploid oat Avena insularis is a close relative of A. magna and A. murphyi and is more plausible than either of these two species as the immediate tetraploid ancestor of A. sterilis and the other hexaploid oats. This report presents the C-banding karyotype of A. insularis. As in A. magna and A. murphyi, the chromosomal complement can be divided into two groups, one predominantly heterochromatic and the other predominantly euchromatic. The heterochromatic chromosome group of A. insularis consists of three pairs of metacentric and four pairs of submetacentric chromosomes. The euchromatic chromosome group consists of one pair of metacentric, five pairs of submetacentric, and one pair of subtelocentric chromosomes.  相似文献   

1B/1R易位系——“84059-4-2”的细胞学鉴定   总被引：8，自引：0，他引：8  

钟少斌  姚景侠 《作物学报》1991,17(5):321-325

利用 C-分带和 N-分带对普通小麦选系“84059-4-2”的根尖细胞染色体进行分析,发现其中的1B 染色体发生了变异。经带型比较,确定该变异染色体由小麦的1B 和黑麦的1R 易位而来,它包括1B 长臂及其着丝粒和1R 短臂。在中国春双端二体1B 与“84059-4-2”的杂种 F_1植株中,发现95.83%的花粉母细胞出现一个异型二价体和一个游离的短  相似文献   

普通小麦与偏凸山羊草代换系的细胞学和RAPD鉴定   总被引：1，自引：0，他引：1       下载免费PDF全文

徐如宏杨英仓  任明见张庆勤 《麦类作物学报》2005,25(4):10-14

为了解偏凸山羊草与普通小麦杂种后代抗白粉病种质BC5-2的遗传组成,对其进行了细胞学和RAPD鉴定。结果表明,BC5-2根尖细胞染色体数目为2n=42,花粉母细胞减数分裂中期(PMC M)染色体构型为2n=21。经核型和C-分带分析初步证明BC5-2为偏凸山羊草的双代换系,其中一对为偏凸山羊草的2MV染色体,另一对为6MV染色体,在BC5-2中被代换的小麦染色体是1B和6A染色体。对BC5-2及其亲本进行RAPD分析,在100个随机引物中有2个引物在BC5-2中扩增出偏凸山羊草的特异DNA带,它们分别记为S2011550、S20031000,进一步证明BC5-2是一个普通小麦与偏凸山羊草的双代换系。  相似文献   

甘蔗异染色质的研究   总被引：2，自引：2，他引：2  

郑成木 《热带作物学报》1994,15(2):39-43

应用Giemsa C一分带技术显示甘蔗染色体异染色质区,结果表明,甘蔗异染色质含量平均为51％。不同品种的异染色质含量差异显著。异染色质在染色体上的分布可分为4种类型,其中最基本且较稳定的类型是着丝点区域为异染色质区。完全异染色质化的染色体常是小型染色体。  相似文献   

3种蝗虫的核型和C-带研究     

杨贤善  任炳忠  鲁莹 《吉林农业大学学报》2012,(3):254-259

研究了中国东北地区槌角蝗科(Gomphoceridae)西伯利亚蝗[Gomphocerus sibiricus sibiricus(L.)]、网翅蝗科(Arcypterinae)大兴安岭异爪蝗(Euchorthippus dahinganlingensis Zhang et Ren)和斑翅蝗科(Oedipodidae)鼓翅皱膝蝗[Angaracris barabensis(Pall.)]的核型和C-带。结果表明:西伯利亚蝗、大兴安岭异爪蝗和鼓翅皱膝蝗的染色体数目分别为2n(♂)=18+XO=19,2n(♂)=16+XO=17,2n(♂)=22+XO=23。  相似文献   

普通小麦-华山新麦草异附加系的分子细胞遗传学研究   总被引：11，自引：1，他引：11  

赵继新  陈新宏  王小利 《西北农林科技大学学报(自然科学版)》2004,32(11):105-108,113

利用荧光原位杂交和染色体C-分带技术,对普通小麦-华山新麦草的异附加系进行了研究。荧光原位杂交结果显示:异附加系H9015-17-1-9,H9017-14-16-5都附加有2条华山新麦草的染色体。对这2个材料和华山新麦草进行染色体C-分带带型比较,初步推断,H9015-17-1-9附加的是Nh5染色体,H9017-14-16-5附加的是Nh6染色体。  相似文献   

普通小麦—大麦异附加系的分子生物学技术鉴定   总被引：1，自引：0，他引：1       下载免费PDF全文

陈新宏  刘淑会  赵继新 《西北农林科技大学学报(自然科学版)》2003,31(6):5-8

用植物染色体C-分带和小麦种子HMW-GS电泳技术,对普通小麦-大麦异附加系进行了鉴定。结果表明,异附加系WB9905和WB9912含有大麦的5H染色体,异附加系WB9906含有大麦的2H染色体。小麦种子HMW-GS电泳结果表明,异附加系WB9905,WB9912和易位系WB9917含有大麦HMW-GS的特征带。  相似文献