共查询到19条相似文献，搜索用时 140 毫秒
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《中国兽医杂志》2015,(6)
通过对重庆某羊场送检病死羊只进行病原菌的分离培养、形态学鉴定、生化鉴定、药敏试验、致病性试验及16S r RNA序列分析。结果从病变组织中分离到1株革兰阳性短棒状杆菌,其对多种抗生素敏感,能够引起小鼠肝脏和肺脏发生病变,PCR扩增出长为1 434 bp的目的序列,已被Gen Bank数据库收录,序列号为KJ842125,将该序列与NCBI中公布的序列进行比对,发现所得序列与化脓隐秘杆菌(Arcanobacterium pyogenes,登陆编号为HQ712123)同源性高达98%,将该序列与其相关性较高菌株的16S r RNA构建系统发育进化树,与化脓隐秘杆菌聚为一簇。本研究为化脓隐秘杆菌致病机理、诊断技术和疾病控制奠定了基础。 相似文献
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从天津地区某猪场病猪肺脏脓肿中分离到一株具有β溶血特性的细菌,经形态学与生化特性分析,初步确定为化脓隐秘杆菌(Arcanobacterium pyogenes),并命名为TJjh1913.利用PCR扩增16S rRNA基因,经Blast序列分析,其序列与GenBank数据库中猪源化脓隐秘杆菌的核苷酸序列同源性高达99.... 相似文献
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本研究旨在分离鉴定来自北京某奶牛场死亡奶牛肺脏的1株疑似病原菌CVCC 3982,并测定其致病性。通过分离培养获得疑似病原菌,采用Biolog鉴定系统和16S rDNA序列分析对其进行了鉴定,人工接种CD-1小鼠测定了其致病性,合成引物对其主要毒力基因进行了检测。结果显示,该疑似病原菌为革兰氏阳性杆菌,β溶血,Biolog鉴定结果显示其为化脓隐秘杆菌,其16S rDNA序列与化脓隐秘杆菌模式菌株NCTC 5224的同源性达100%,系统发育分析显示其与化脓隐秘杆菌处于同一分支。腹腔注射该菌可致小鼠死亡。分离菌株基因组中含有溶血素(PLO)基因,神经氨酸酶H(NanH)基因,神经氨酸酶P(NanP)基因,菌毛基因(fimA、fimC和fimE),但缺失胶原结合蛋白(CbpA)基因和菌毛fimG基因。结果表明该分离菌株为化脓隐秘杆菌且具有致病性。 相似文献
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副猪嗜血杆菌的分离鉴定及16S rRNA序列分析 总被引：1，自引：1，他引：0
从云南某规模化养猪场病猪肺脏分离到1株革兰氏阴性小杆菌,经细菌生化鉴定、PCR鉴定和16S rRNA序列比对鉴定为副猪嗜血杆菌。抗生素药物敏感试验结果表明,分离菌株对四环素、红霉素、氯霉素、头孢噻吩高敏;对庆大霉素、氧氟沙星、诺氟沙星中敏;对磺胺甲唑耐药。16S rRNA分析结果表明,该分离株与GenBank中的Hps参考株AB078973(基因登录号)同源性为100%,将分离菌株鉴定为副猪嗜血杆菌。16S rRNA遗传进化关系表明,分离株与副猪嗜血杆菌3株血清5型参考株AB078972、AB078973、AB078974的16S rRNA序列位于一个分支上,遗传进化关系最近,它们之间的核苷酸同源性在99.0%~99.4%之间,初步鉴定为血清5型副猪嗜血杆菌,致病性试验结果表明,分离菌株对小白鼠有强致病性,命名为YN-1株。 相似文献
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从3日龄健康小鸡肠道中分离到1株乳杆菌,用PCR方法从分离菌株扩增16S rRNA基因,获得大小为1340 bp的DNA片段,该片段的核酸序列已提交GenBank,登录号为EU290749。将分离株的16S rRNA基因核苷酸序列与GenBank上其它乳杆菌进行同源性分析并建立进化树。结果表明,分离株的16S rRNA基因核苷酸序列与NCBI公布的鼠约氏乳杆菌分离株(AB295648)的同源性为99.6%,因此该分离菌株被鉴定为鸡约氏乳杆菌(chicken Lactobacillus johnsonii),命名为HN/0711。 相似文献
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为了调查产后3周内健康和子宫炎奶牛子宫中细菌分离情况,尤其是化脓隐秘杆菌及其毒力基因的分布情况。本试验采集了产后3周内15头健康奶牛和11头患子宫炎奶牛的子宫分泌物进行细菌分离鉴定,并对分离到的化脓隐秘杆菌进行毒力基因的检测。结果表明,大肠杆菌、化脓隐秘杆菌、芽孢杆菌是产后3周内子宫分泌物最常分离培养的细菌;从15头健康奶牛和11头患病奶牛的子宫分泌物中分离到17株化脓隐秘杆菌,所有菌株均携带plo基因和fimA基因;携带多种毒力基因的化脓隐秘杆菌在健康和子宫炎奶牛中均可以分离到。本试验为进一步研究化脓隐秘杆菌对子宫内膜的致病机理提供参考依据。 相似文献
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四川某商品猪场仔猪出现以体温升高,背、腹和腿部皮肤发绀,淋巴结出血肿大,肺、脾脏出血、化脓为主要特征的疾病。通过对分离细菌的纯化培养、形态学观察、生化试验、致病性试验和药物敏感性试验,鉴定出一株致病菌。应用通用引物对其16S rRNA基因进行克隆和序列分析,序列在NCBI上进行BLAST比对,选择同源性较高的棒状杆菌属中25株不同种的菌株16S rRNA序列进行比对分析,构建遗传进化树。分离纯化的细菌在鲜血琼脂平板厌氧条件下生长良好,为革兰氏染色阳性杆状菌,能发酵部分糖类,能致死小白鼠,对头孢类抗生素敏感,16S rRNA序列与棒状杆菌属细菌的同源性最高,在78.2%~98.0%之间,遗传进化树分析结果表明,该菌株与棒状杆菌属细菌属于同一分类群,分离菌鉴定为棒状杆菌。 相似文献
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为了解引起山羊皮下脓肿的主要病原,本研究从四川乐至县、金堂县和蓬溪县3个山羊养殖场无菌采集患皮下脓肿病羊脓液样本19份,通过细菌分离培养、生化试验及特异性PCR方法对其病原进行检测和鉴定,并通过药敏试验测定分离病原的药物敏感性。结果显示,PCR扩增法共检测出病原菌19株,其中伪结核棒状杆菌、金黄色葡萄球菌及化脓隐秘杆菌分离率分别为57.9%(11/19)、26.3%(5/19)及15.8%(3/19);采用细菌分离培养法分离到3种形态不同的病原菌共计17株,其中革兰氏阳性短杆菌经鉴定为伪结核棒状杆菌,分离率57.9%(11/19),革兰氏阳性球菌经鉴定为金黄色葡萄球菌,分离率为21.1%(4/19),革兰氏阳性杆菌经鉴定为化脓隐秘杆菌,分离率为10.5%(2/19),PCR鉴定较细菌分离培养法灵敏度更高。3种病原菌对丁胺卡那、氟苯尼考等多种药物敏感。结果表明,伪结核棒状杆菌是引起该地区山羊皮下脓肿的主要病原,金黄色葡萄球菌和化脓隐秘杆菌同样可引起山羊皮下脓肿,特异性PCR方法较细菌分离培养更为高效、准确。本研究结果可为后续进一步分析其生物学特性,以及四川地区山羊皮下脓肿的综合性防制提供依据。 相似文献
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猪化脓隐秘杆菌的分离与鉴定 总被引：3，自引：1，他引：2
从一例肝、肺化脓性结节,脾出血性肿大的病猪体内分离到一株革兰氏阳性、细长、形态不规则的杆菌,该菌在TSA平板、普通绵羊血琼脂、巧克力平板上生长缓慢,在普通绵羊血琼脂上呈α-溶血。对该菌的16S rDNA进行PCR扩增、测序、比较,结果显示该菌与化脓隐秘杆菌的16S rDNA有99%的同源性,进一步用API生化鉴定,结果也与其相符。该菌在24~52 h内100%致死小白鼠,腹腔接种新西兰兔,接种兔72 h内死亡,表明该菌为致病性细菌。 相似文献
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Rapid identification of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes by fluorescence in situ hybridization (FISH) 总被引：1，自引：0，他引：1
Fluorescence in situ hybridization (FISH) has been reported to be an easy and rapid identification method for many human pathogens, but applications for common veterinary pathogens are lacking. Gene probes for FISH of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes were designed to provide probes for a specific identification of these bacteria from cultures. Specific FISH probes for these species have so far not been published. Both probes recognized all isolates of the target species correctly. With the S. uberis probe SUB 196 no false-positive results were obtained for reference strains as well as for clinical isolates. Probe APYO 183 for A. pyogenes produced false-positive reactions with so far rarely described Arcanobacterium species from animals at standard hybridization conditions. In order to avoid any incorrect classifications of microorganisms as A. pyogenes, two non-labelled competitor probes were designed and successfully evaluated. 相似文献
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为研究牛源的化脓隐秘杆菌溶血素(PLO)生物学功能,本研究应用PCR方法扩增牛源化脓隐秘杆菌(A.pyogenes)PLO全基因序列,通过生物信息学方法分析其与猪源A.pyogenes的PLO蛋白差异,并构建了溶血功能区重组表达质粒pQE30-PLO585,在E.coli XL1Blue中用IPTG诱导表达。结果表明,扩增到PLO蛋白基因ORF为1 605 bp,编码535个氨基酸,与猪源PLO的核苷酸序列同源性为97.4%,氨基酸同源性为97.2%,在生物学活性功能区没有发生改变。表达的PLO蛋白溶血功能区重组蛋白能够被阳性血清识别,而且具有溶解绵羊红细胞的活性,产生β溶血现象。本研究获得了牛源A.pyogenes截短重组PLO蛋白,并证明PLO具有β溶血功能与较好的抗原性。 相似文献
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Manuel Chirino-Trejo Murray R Woodbury Fei Huang 《Journal of zoo and wildlife medicine》2003,34(3):262-268
Bacterial cultures from 32 living and dead farmed white-tailed deer (Odocoileus virginianus) with necrobacillosis yielded Fusobacterium necrophorum from nine individuals, F. varium from six individuals, and Arcanobacterium pyogenes from 16 individuals. The isolates were characterized biochemically using automated identification systems. Gram-stained smears suggested the presence of Fusobacterium spp. in eight cases from which organisms were not cultured. Minimum inhibitory concentration determinations in 23 strains of gram-negative anaerobic bacteria detected resistance to enrofloxacin and clindamycin. Enrofloxacin resistance was detected in A. pyogenes isolates, and although biochemical profiling indicated that the deer strains of A. pyogenes could be grouped, it is uncertain whether these biochemical characteristics correlate with antigenic or virulence factors. Deer-specific or autogenous vaccines may provide a useful alternative to generic vaccines. 相似文献
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Markowska-Daniel I Urbaniak K Stepniewska K Pejsak Z 《Polish journal of veterinary sciences》2010,13(1):29-36
Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test. beta-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied. 相似文献
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为探明贵州省某猪场引起猪体表脓肿的原因,本研究对该猪场脓肿部位的脓汁进行细菌分离培养,并对分离所得的细菌进行革兰氏染色镜检、生化试验、药敏试验、16S rDNA序列分子分析及动物感染试验。结果显示,从脓汁中成功分离到了3株菌落形态不一的菌株,分别为金黄色葡萄球菌、化脓隐秘杆菌、停乳链球菌类马亚种,根据分离地点和时间将其分别命名为GZGP2018-1、GZGP2018-2和GZGP2018-3;GZGP2018-1菌株与NCBI上金黄色葡萄球菌的同源性高达99.9%,GZGP2018-2菌株与NCBI上化脓隐秘杆菌的同源性高达99.9%,GZGP2018-3菌株与NCBI上停乳链球菌类马亚种的同源性高达100%;3株分离菌株对头孢拉定、环丙沙星、磺胺间甲氧嘧啶和氟苯尼考较敏感,对青霉素类药物和红霉素耐药;3株分离菌株对试验小鼠均具有致死性。本研究为该猪场猪体表脓肿的发病原因、实验室诊断方法及日常防控提供了理论参考。 相似文献
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Ohba T Shibahara T Kobayashi H Kubo M Takashima A Imai S Murakami S Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):449-453
A 6-month-old barrow presented with lethargy, inappetence and dysstasia. At necropsy, multiple coalescing hemorrhagic foci were detected in the margins of the spleen. Gram-positive bacilli were isolated from the spleen, kidney, muscle and liver. Comparative 16S rDNA gene sequencing analysis of the isolates (TO16177) revealed that they would be the same species of unpublished Arcanobacterium species strain HJ57-14E (accession no. gi 18873551) (99.7% similarity based on a comparison of 675 bp). Histologic examination of the splenic tissue sections revealed extensive necrosis and inflammation, and gram-positive bacilli were discernible. Multifocal necrosis was also detected in the liver. Immunohistochemically, the isolates were cross-reacted with polyclonal antibodies against Arcanobacterium pyogenes and Actinomyces naeslundii, and the reaction was strong for the latter. Similar reactions were found in the suppurative lesions of the tonsil, and occasionally in the spleen and lymph nodes. The present results indicate that the unpublished Arcanobacterium species induced multiple organ failure accompanied by acute hemorrhagic necrotizing splenitis in this growing-finishing pig. 相似文献
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Arcanobacterium pyogenes is a normal inhabitant of the mucous membranes of domestic animals, such as cattle, sheep, swine, and goats. It is also an opportunistic pathogen in these animals, where it causes a variety of purulent infections involving the skin, joints, and visceral organs. Two recent cases of isolation of A. pyogenes from companion animals are reported. In the first case, a cat presented with a chronic otitis externa, from which A. pyogenes was isolated in pure culture. The second case involved a dog with a urinary tract infection, where A. pyogenes was isolated from urine as the predominant bacterial species. In both cases, the A. pyogenes isolates were presumptively identified by macrobiochemical tests, and then their identities were confirmed by polymerase chain reaction analysis and 16S rRNA gene sequencing. 相似文献