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Daub Matthias Hakl Ulrike Molendijk Leendert PG Schomaker Corrie Been Thomas H van Beers Thea G Jivishova Sevda Jivishov Emil Keusgen Michael Bluemel Roman Fischer Daniel Grundler Florian MW Reuther Marie Cappel Sabrina Bauer Harald Lang Christian Watrin Cliff den Nijs Loes JMF Van Bruggen Anne-Sophie Karssen Gerrit Kiewnick Sebastian Büchler Urs Roth Irma Frey Jürg 《植物病害和植物保护杂志》2015,122(4):189-193
Journal of Plant Diseases and Protection - 相似文献
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André Gomes Coelho Souza Sonia Herrero Luiz Antonio Maffia Margaret Elizabeth Daub 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(2):235-238
Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈108/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %). 相似文献
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Blessing Mabate Chantal Dsire Daub Samkelo Malgas Adrienne Lesley Edkins Brett Ivan Pletschke 《Marine drugs》2021,19(1)
Fucoidans are complex polysaccharides derived from brown seaweeds which consist of considerable proportions of L-fucose and other monosaccharides, and sulphated ester residues. The search for novel and natural bioproduct drugs (due to toxicity issues associated with chemotherapeutics) has led to the extensive study of fucoidan due to reports of it having several bioactive characteristics. Among other fucoidan bioactivities, antidiabetic and anticancer properties have received the most research attention in the past decade. However, the elucidation of the fucoidan structure and its biological activity is still vague. In addition, research has suggested that there is a link between diabetes and cancer; however, limited data exist where dual chemotherapeutic efforts are elucidated. This review provides an overview of glucose metabolism, which is the central process involved in the progression of both diseases. We also highlight potential therapeutic targets and show the relevance of fucoidan and its derivatives as a candidate for both cancer and diabetes therapy. 相似文献
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ABSTRACT Transformation of plants with the nucleocapsid (N) gene of Tomato spotted wilt tospovirus (TSWV) provides resistance to disease development; however, information is lacking on the response of plants to natural inoculum in the field. Three tobacco cultivars were transformed with the N gene of a dahlia isolate of TSWV (TSWV-D), and plants were evaluated over several generations in the greenhouse. The resistant phenotype was more frequently observed in 'Burley 21' than in 'KY-14' or 'K-326', but highly resistant 'Burley 21' transgenic lines were resistant to only 44% of the heterologous TSWV isolates tested. Advanced generation (R(3) and R(4)) transgenic resistant lines of 'Burley 21' and a 'K-326' F(1) hybrid containing the N genes of two TSWV isolates were evaluated in the field near Tifton, GA, where TSWV is endemic. Disease development was monitored by symptom expression and enzyme-linked immunosorbent assay (ELISA) analysis. Whereas incidence of TSWV infection in 'Burley 21' susceptible controls was 20% in 1996 and 62% in 1997, the mean incidence in transgenic lines was reduced to 4 and 31%, respectively. Three transgenic 'Burley 21' lines were identified that had significantly lower incidence of disease than susceptible controls over the two years of the study. In addition, the rate of disease increase at the onset of the 1997 epidemic was reduced for all the 'Burley 21' transgenic lines compared with the susceptible controls. The 'K-326' F(1) hybrid was as susceptible as the 'K-326' nontransformed control. ELISA analysis demonstrated that symptomless plants from the most resistant 'Burley 21' transgenic lines accumulated detectable nucleocapsid protein, whereas symptomless plants from more susceptible lines did not. We conclude that transgenic resistance to TSWV is effective in reducing incidence of the disease in the field, and that accumulation of transgene protein may be important in broad-spectrum resistance. 相似文献
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Breeding transgressive lines of pepper for resistance to Phytophthora capsici in a recurrent selection system 总被引：1，自引：0，他引：1
Summary In order to increase the resistance level of pepper (Capsicum annuum) to Phytophthora capsici the main sources of genetic resistance were intercrossed. The parents included 7 varieties from different origins of partial resistance to P. capsici and to other soilborne pathogens. Two cycles of recurrent selection were performed by 7 plant breeders who screened the plants at two developmental stages with various isolates and at two temperatures. Analysis of data revealed a strong influence of the testing conditions on the evaluation of the plants. The level of resistance of the progenies further depended on the pathogen isolate used to screen the plants. Improvement of the whole population was more significant during the first cycle and lines with enhanced level of resistance as compared to the parents i.e. transgressive lines were fixed from the two cycles. These lines were resistant to much higher inoculum concentrations than the original parents and the expression of resistance was stable at high temperature (32°C). These new sources of polygenic resistance were included into a new breeding program to improve the agronomic characters. 相似文献
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Daub Matthias Hakl Ulrike Maressa Beira Hailu Dehne Heinz-Wilhelm Hallmann Johannes Kiewnick Sebastian Eberlein Caroline Heuer Holger Moos Jan H Paulsen Hans M Westphal Andreas Sturhan Dieter Kiewnick Sebastian Wolf Stefanie Bühlmann Andy Frey Jürg Nuaima Rasha Heuer Holger Westphal Andreas 《植物病害和植物保护杂志》2014,121(4):187-189
Journal of Plant Diseases and Protection - 相似文献
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Ghedin E Wang S Spiro D Caler E Zhao Q Crabtree J Allen JE Delcher AL Guiliano DB Miranda-Saavedra D Angiuoli SV Creasy T Amedeo P Haas B El-Sayed NM Wortman JR Feldblyum T Tallon L Schatz M Shumway M Koo H Salzberg SL Schobel S Pertea M Pop M White O Barton GJ Carlow CK Crawford MJ Daub J Dimmic MW Estes CF Foster JM Ganatra M Gregory WF Johnson NM Jin J Komuniecki R Korf I Kumar S Laney S Li BW Li W Lindblom TH Lustigman S Ma D Maina CV Martin DM McCarter JP McReynolds L Mitreva M Nutman TB 《Science (New York, N.Y.)》2007,317(5845):1756-1760
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design. 相似文献
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Summary Two cycles of recurrent selection were performed in a population including 7 small fruited varieties of pepper (Capsicum annuum) with low resistance level to Verticillium and resistance to other soilborne pathogens. The collaboration of 7 plant breeders in this work and the use of 2 artificial methods of inoculation allowed screening of numerous plants for resistance to V. dahliae under different conditions. The population was simultaneously improved for resistance to V. dahliae and for resistance to Phytophthora capsici, and at each cycle, intercrosses were performed between all these plants. The general level of resistance to V. dahliae increased continuously during the 2 cycles of seles of selection. Selfed progenies of plants from the 2 cycles displayed a higher resistance than the original parents, whatever the method of inoculation used. This gave evidence of transgressions for resistance. Crosses involving parents that did not seem resistant by themselves also produced transgressive lines, indicating that resistance genes were present but not active in these varieties. The transgressive lines with polygenic resistance to V. dahliae were included into a further breeding program to introduce genes of agronomic value. 相似文献