排序方式： 共有8条查询结果，搜索用时 15 毫秒
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Daub Matthias Hakl Ulrike Molendijk Leendert PG Schomaker Corrie Been Thomas H van Beers Thea G Jivishova Sevda Jivishov Emil Keusgen Michael Bluemel Roman Fischer Daniel Grundler Florian MW Reuther Marie Cappel Sabrina Bauer Harald Lang Christian Watrin Cliff den Nijs Loes JMF Van Bruggen Anne-Sophie Karssen Gerrit Kiewnick Sebastian Büchler Urs Roth Irma Frey Jürg 《植物病害和植物保护杂志》2015,122(4):189-193
Journal of Plant Diseases and Protection - 相似文献
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Sebastian Kiewnick Martijn Holterman Sven van den Elsen Hanny van Megen Juerg Ernst Frey Johannes Helder 《European journal of plant pathology / European Foundation for Plant Pathology》2014,140(1):97-110
Root-knot nematodes (Meloidogyne spp.) are important pests of numerous crops worldwide. Some members of this genus have a quarantine status, and accurate species identification is required to prevent further spreading. DNA barcoding is a method for organism identification in non-complex DNA backgrounds based on informative motifs in short DNA stretches (≈600 bp). As part of the EU 7th Framework project QBOL, 15 Meloidogyne species were chosen to compare the resolutions offered by two typical DNA barcoding loci, COI and COII, with the distinguishing signals produced by two ribosomal DNA genes (small and large subunit rDNA; SSU?≈?1,700 and LSU?≈?3,400 bp). None of the four markers distinguished between the tropical species Meloidogyne incognita, M. javanica and M. arenaria. Taking P ID (Liberal) values ≥0.93 as a measure for species delimitation, the four mtDNA and rDNA markers performed well for the tropical Meloidogyne species complex, M. enterolobii, M. hapla, and M. maritima. Within cluster III A (Holterman et al. Phytopathology, 99, 227–235, 2009), SSU rDNA did not offer resolution at species level. Both mtDNA loci COI and COII did, whereas for LSU rDNA a longer fragment (≥700 bp) is required. The high level of mitochondrial heteroplasmy recently reported for M. chitwoodi (Humphreys-Pereira and Elling Nematology, 15, 315–327, 2013) was not found in the populations under investigation, suggesting this could be a regional phenomenon. For identification of RKNs, we suggest the combined use of SSU rDNA with one of three other markers presented here. 相似文献
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Daub Matthias Hakl Ulrike Maressa Beira Hailu Dehne Heinz-Wilhelm Hallmann Johannes Kiewnick Sebastian Eberlein Caroline Heuer Holger Moos Jan H Paulsen Hans M Westphal Andreas Sturhan Dieter Kiewnick Sebastian Wolf Stefanie Bühlmann Andy Frey Jürg Nuaima Rasha Heuer Holger Westphal Andreas 《植物病害和植物保护杂志》2014,121(4):187-189
Journal of Plant Diseases and Protection - 相似文献
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Journal of Plant Diseases and Protection - Root-knot nematodes (Meloidogyne spp.) cause extensive damage to tomato and cucurbit crops in protected cultivation systems. An environmentally benign... 相似文献
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Andrea Braun-Kiewnick Sebastian Kiewnick 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(2):271-283
Plant-parasitic nematodes can cause significant damage to agricultural crops and forests worldwide, resulting in major economic losses. Some nematode species do not occur in all areas and are regulated as quarantine organisms. To avoid introduction and spread of these organisms, fast, simple and reliable detection and identification methods are needed, that help plant diagnostic services such as reference centres or national plant protection organizations (NPPOs) to rapidly identify suspicious nematodes. Real-time PCR is one of the fastest, most sensitive and reliable methods to fulfil this task. It is a DNA-based method that is easy to learn with the only requirement of having a specific thermocycler (Real-time Platform) and the appropriate chemistry. Real-time PCR provides very sensitive detection and species-specific identification with the potential to quantify target organisms if required. Following DNA extraction, results can be seen in 1–3 h and management decisions applied. Real-time PCR can be used for high-throughput analysis of many samples and in some cases for multiplexing, allowing for identification of more than one species in a single reaction. Over the past 15 years, real-time PCR methods have been developed for the main plant-parasitic nematodes, in particular the regulated species. This paper reviews the achievements in plant nematology diagnostics using real-time PCR as the method of choice for fast and reliable detection, identification and even quantification of plant parasitic nematodes. 相似文献
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Storelli Alan Minder Alexandra Keiser Andreas Kiewnick Sebastian Daub Matthias Mahlein Anne-Katrin Schumann Mario Beyer Werner 《植物病害和植物保护杂志》2021,128(5):1303-1311
Journal of Plant Diseases and Protection - Ditylenchus dipsaci is an economically important plant-parasitic nematode affecting European sugar beets. To date, no sugar beet cultivars carrying... 相似文献
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Zusammenfassung Paecilomyces lilacinus Stamm 251 (PL251) ist ein fakultativer, eipathogener Pilz, der im Rahmen einer integrierten Bekämpfung zur Kontrolle des Zuckerrübenzystennematoden Heterodera schachtii eingesetzt werden kann. In einem Feldversuch sollte untersucht werden, ob sich der Antagonist unter Freilandbedingungen in einem Bodenökosystem etablieren kann und welche Parameter seine Persistenz beeinflussen. PL251 wurde in kommerzieller Formulierung (BIOACT® WG) zur Zuckerrübensaat mit einer Aufwandmenge von 4 kg Produkt pro Hektar appliziert und in den Boden eingearbeitet. Anschließend wurde die Dichte des Antagonisten im Boden 0, 50, 90 Tage nach der Applikation sowie zur Ernte untersucht. Es konnte gezeigt werden, dass unmittelbar nach der Applikation die Verteilung von PL251 im Boden sehr heterogen und die Dichte deutlich niedriger als erwartet war. Innerhalb von 90 Tagen nahm die Dichte unabhängig von der Ausgangskonzentration um durchschnittlich mehr als 90% ab. Zur Ernte konnte PL251 nicht mehr aus der Rhizosphäre von Zuckerrüben rückisoliert werden. Der im Verlauf der Zeit festgestellte Populationsabfall war unabhängig von der räumlichen Verteilung und der Populationsentwicklung von H. schachtii. Es konnte somit demonstriert werden, dass bei der Anwendung des antagonistischen Pilzes PL251, aufgrund der geringen Persistenz unter Freilandbedingungen, die Wahrscheinlichkeit, das Risiken für die Umwelt bestehen, als äußerst gering einzustufen ist. 相似文献
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